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A membrane protein gp64 inhibits development of Polysphondylium

Research Project

Project/Area Number 12640643
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

OCHIAI Hiroshi  Hokkaido Univ., Grad. School of Sci, Prof., 大学院・理学研究科, 教授 (10002122)

Co-Investigator(Kenkyū-buntansha) SAITO Tamao  Hokkaido Univ., Grad. School of Sci, Inst., 大学院・理学研究科, 助手 (30281843)
KATO Atsushi  Hokkaido Univ., Grad. School of Sci, Asso. Prof., 大学院・理学研究科, 助教授 (90177428)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordscellular slime mold / Polysphondylium / membrane protein / stalk differentiation / ecmB / gp64 / Polysphondylium / 細胞性粘歯 / 細胞膜タンパク質 / Polysphond / ylium / 分枝 / 形質転換
Research Abstract

To examine the function of the membrane protein gp64 of Polysphondylium, a over expresser of gp64 was examined. In the light, when the wild-type and the overexpresser, both the strains developed into normal fruiting bodies. In the dark, the wild type developed lately but became fruiting bodies at 48 hr. In contrast the overexpresser the almost cells did not stream together, except migrating cylindrical slugs.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] M.Maeda: "Development change in the spatial expression of genes involved in myosin function in Dictyostelium"Dev. Biol.. 223. 114-119 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Tsujioka: "Spatial expression patterns of genes involved in cyclic AMP responses in Dictyostelium discoideum"Develop. Growth Differ.. 43. 275-283 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Funamoto: "cAMP dependent kinase regulates Polysphondylium pallidum development"Differentiation. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N.Van Driessche: "A transcriptional profile of multicellular development in Dictyostelium discoideum"Development. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N.Takaoka: "Vegetatively regulated elements of the Polysphondylium pallidum gp64 promoter"Biochimica Biophysica Acat. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Takeda: "A novel Dictyostelium Cdk8 is required for aggregation, but dispensable for growth"Develop. Growth Differ.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mineko Maeda: "Developmental change in the spatial expression of genes involved in myosin function in Dictyostelium"Dev. Biol.. 223. 114-119 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masatsune Tsujioka: "Spatial expression patterns of genes involved in cyclic AMP responses in Dictyostelium discoideum"Develop. Growth Differ.. 43. 275-283 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Satoru Funamoto: "cAMP dependent kinase regulates Polysphondylium pallidum developmenet"Differentiation. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nancy Van Driessche: "A transcriptional profile of multicellular development in Dictyostelium discoideum"Development. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Naohisa Takaoka: "Vegetatively regulated elements of the Polysphondylium pallidum gp64 promoter"Biochimica Biophysica Acta. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kosuke Takeda: "A novel Dictyostelium Cdk8 is required for aggregation, but dispensable for growth."Dev. Growth Differ.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Maeda: "Developmental change in the spatial expression of genes involved in myosin function in Dictyostelium"Developmental Biology. 223. 114-119 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] M. Tsujioka: "Spatial expression patterns of genes involved in cyclic AMP responses in Dictyostelium discoideum"Develop. Growth Differ.. 43. 275-283 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S. Funamoto: "cAMP dependent kinase regulates Polysphondylium pallidum development"Differentiation. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] N. Van Driessche: "A transcriptional profile of multicellular development in Dictyostelium discoideum"Development. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] N. Takaoka: "Vegetatively regulated elements of the Polysphondylium pallidum gp64 promoter"Biochimica Biophysica Acat.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] K. Takeda: "A novel Dictyostelium Cdk8 is required for aggregation, but dispensable for growth"Develop. Growth Differ.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Tsujioka: "Spatial Expression Patterns of Genes Involved in cAMP-Responses in Dictyostelium Development"Developmental,Growth and Differentiation. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Maeda: "Developmental Changes in the Spatial Expression of Genes Involved in Myosin Function in Dictyostelium"Developmental Biology. 223. 114-119 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 落合廣: "「行動の分子生物学-山元大輔偏」分担執筆,細胞性粘菌の行動と分子"シュプリンガー・フェアラーク東京株式会社. 10 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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