| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Research Abstract |
The purpose of this research is to understand the evolutionary history of the reproductive endocrine system of vertebrates by characterization of reproduction-related homones and their receptors. During this study we have established breeding colony of a reptile, and characterized a novel GnRH physiological function.
1. Establishment of breeding colony of Leopard Gecko (Eublephalis macularius) as a reptilian model animal : To promote systematic research in reptilian reproductive endocrine syste, we have tried to select from various reptile species for a loboratory animal model. Among them, leopard gecko was most suitable because of their unique sex determinating system and easy handling. Now, our leopard gecko colony has been bred for 4 years and the current colony size is about 70 heads.
2. Molecular characterization of Reproduction related hormone receptor in birds and reptiles : Using our newly established leopard gecko breeding colony, we have characterize steroid hormone receptors (
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estrogen, androgen and progesterone receptors) and GnRH receptor. So far, partial cDNA sequences of these receptors are characterized, and their full sequences are GnRH receptor. So far, partial cDNA sequences of these receptors are characterized, and their full sequences are under the characterization by RACE and ligand binding activity.
3. Regulating activity of colony-forming efficiency of GnRH : GnRH promotes GTH secretion from pituitary. However there is increasing evidence for extra-pituitary effects on number of peripheral tissues. In this study, we found that GnRH regulates the colony forming efficiency with high secsitivity (10^<-15>-10^<-12>M). This activity was also greatly effected by auto-and/or paracrine activity of the cell line.
4. Molecular characterization of Guinea pig GnRH receptor : Guinea pig GnRH is predicted to have a unique structure among all known forms of GnRH molecule. In this study, we characterized mRNA of GnRH receptor from the guinea pig pituitary. Characterized GnRH receptor is belong to type I GnRH receptor group, and shows similar ligand binding characteristics to that of the rat GnRH receptor. Less
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