• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Ultrastructural and microbiological research of the double fertilization in angiosperm

Research Project

Project/Area Number 12640646
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionThe University of Tokyo

Principal Investigator

KUROIWA Haruko  The University of Tokyo, Graduate School of Science, Assistant, 大学院・理学系研究科, 助手 (50313200)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsPelargonium zonale / double fertilization / egg cell / giant mitochondria / mitochondrial DNA / amyloplast / zygote / ミトコンドリアDNA / 原色素体
Research Abstract

Changes in the distribution of organelles and organelle-DNA in Pelargonium zonale from the mature egg cell stage to the first zygotic division during the early stages of embryogenesis were investigated using electron microscopy and fluorescence microscopy. The mature egg is a large, polarized bulbous-shaped cell tapering toward its micropylar end. The wide chalazal region has a large nucleus that is surrounded by cytoplasm containing many giant mitochondria and large amyloplasts. The mitochondria contain a large amount of mitochondrial DNA (mt-DNA) and appear as long stretched rods or complex rings, sometime consisting of several concentric or half-concentric circles in sections. The tme of pollination to cell fusion is approximately 6 to 9 h and is 20 to 24 h until the first zygotic division. The changes in the zygote and its organelles preparatory to division occur in 3 stages. At stage 1 (6-9 h after pollination), cell fusion occurs and the zygote bigins to elongate. Many vacuoles o … More f varying size appear surrounding the nucleus. At stage 2 89-15 h), the zygote nucleus migrates to a central position in the cell and the mitochondria form a single ring that becomes either irregularly crushed or appears as long thin strings. Amyloplasts exhibit a gradual decrease in the number of starch grains. At stage 3 (15-20 h), the vacuoles disappear, except for a few that remain in the micropyolar region and cell size decreases. Mitochondria become short, fine strings or small rings. Amyloplasts with starch grains are no longer observed, but are transformed into large proplastids. Following the first division of the zygote approximately equal-sized apical and basal cells are formed. Short rod-shaped or small ring-shaped mitochondria are randomly distributed near the nucleus of the apical cell, whereas mitochondria in the basal cell are long and rod-shaped. Inthe electron microscope, two types of plastids can be distinguished : dark oval plastids originating from the sperm cell, which are observed in both apical celland basal cell, and others with a less dense, amorphous matrix, believed to originate from egga amyloplasts, which are unevenly distributed in the micropylar region of the basal cell. fluorometry using video-intensified microscope photon counting system (VIMPICS) reveals that, correlated with changes in mitochondrial morphology, DNA amount within the mitochondrion decreases linearly during these stages. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Suzuki, L. et al.: "A zygote-specific protein with hydroxyproline-rich glycoprotein domains and lectin-like domains involved in the assembry of the cell wall of Chlamidomonous re"J. Phycol.. 36. 571-583 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Higashiyama, T. et al.: "Explosive discharge of pollen tube contents in Torenia foumieri"Plant Physiol.. 122. 11-13 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saito, C., et al.: "Unequal distribution of DNA-containing organelles in generative and sperm cells of Erythrina crista-galli (Fabaceae)"Sexual Plant Reproduction. 12. 296-301 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagata, N. et al.: "Unique positioning of mitochondria in developing microspores and pollen grains in Pharbitis nil : mitochondria cover the nuclear surface at specific developmental stages"Protoplasma. 213. 74-82 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Inada, N., et al.: "Senescence in the nongreening region of the rice (Oriza sativa) coleoptile"Protoplasma. 214. 180-193 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mori, T., et al.: "Visualization of FtsZ Rings in Plastids of the Microspore in Lilium longiflorum"Cytologia. 66,No.1. 113-115 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mori, T., et al.: "Bacterial chloroplast-dividing ring"Plant Cell Physiology. 44. 555-559 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuroiwa, H., et al.: "Multiple FtsZ Rings in a Pleomorphic Chloroplast in Embryonic Cap Cells of Pelargonium zonale"Cytologia. 66,No.2. 227-233 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Higashiyama, T., et al.: "Pollen tube attraction by the synergid cell"Science. 293. 1480-1483 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuroiwa, H., et al.: "Chloroplast division machinery as revealed by immunofluorescence and electron microscopy"Planta. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuroiwa, H., et al.: "Pelargonium embryogenesis : Cytological investigations of organelles in the early embryogenesis from the egg to the two-celled embryo"Sexual Plant Reproduction. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuroiwa, T. et al.: "Kluwer Academic Publisher"Inheritance of cytoplasmic traits-embryological perspectives. 14 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 黒岩晴子: "朝倉植物生理学講座第4巻 「成長と分化-受粉と受精」 (福田裕穂編)"朝倉書店. 11 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki L, Woessner J. P, Uchida H, Kuroiwa H, Uasa Y, Waffenschmidt S, Goodenough U. W, and Kuroiwa T: "A zygote-specific protein with hidroxyproline-rich glycoprotein comains and lectin-like domains involved in the assaembry of the cell wall of Chlamidomonous reinhardtill"J. Phycol. 36. 571-583 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Higashiyama T, Kuroiwa H, Kawano S, and Kuroiwa T: "Explosive discharge of pollen tube contents in Toreina fournieri"Plant Physiol. 122. 11-13 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saito C, Nagata N, Sakai A, Kuroiwa H, and Kuroiwa T: "Unequal distribution of DNA-containing organelles in generative and sperm cells of Erythrina crista-galli (Fabaceae)"Sexual Plant Reprod. 12. 296-301 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagata N, Saito C, Sakai A, Kuroiwa H, and Kuroiwa T: "Unique positioning of mitochondria in developing microspores and pullen grains in Pharbitis nil : mitochondria cover the nuclear surface at specific developmental stages"Protoplasma. 213. 74-82 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Inada N, Sakai A, Kuroiwa H, and Kuroiwa T: "Senescence in the nongreening region of the rice (Oliza sativa) coleoptile"Protoplasma. 214. 189-193 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mori T, Takahara M, Miyagishima S, Kuroiwa H, and Kuroiwa T: "Visualization of FtsZ rings in plastids of the microspore in Lilium longiflorum"Cytologia. 66. 113-115 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mori T, Kuroiwa T, Takahara M, Miyagishima S, and Kuroiwa T: "Visualization of an Frs ring inchloroplasts of Lilium longiflorum Leaves"Plant Cell Physiol. 42. 555-559 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuroiwa H, Mori T, Takahara M, Miyagishima S, and Kuroiwa T: "Multiple FtsZ ring in a pleomorphic chloroplast in embryonic cap cells of Pelargonium zonale"Cytologia. 66. 227-233 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Higashiyama T, Yabe S, Sasaki N, Nishimura Y, Miyagishima S, Kuroiwa H, and Kuroiwa T: "Pollen tube attraction by the synergid cell"Science. 293. 1480-1483 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuroiwa H, Mori T, Takahara M, Miyagishima S, and Kuroiwa T: "Chloroplast division machinery as revealed by immunofluorescence and electron microscopy"Planta. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuroiwa H, Nishimura Y, Higashiyama T, and Kuroiwa T: "Pelargonium embryogenesis : Cytological investigations of organelles in theearly embryogenesis from the egg to the two-celled embryo"Sexual Plant Reproduction. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuroiwa T, Kuroiwa H, Miyagishima S, and Nishimura Y: "Inheritance of cytoplasmic traits-embryological perspectives"Kluwer Academic Pulisher. 509-523 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mori, T., et al.: "Visualization of FtsZ Rings in Plastids of the Microspore in Lilium Longiflorum"Cytologia. 66 No.1. 113-115 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mori, T., et al.: "Bacterial chloroplast-dividing ring"Plant Cell Physiology. 44. 555-559 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kuroiwa, H., et al.: "Multiple FtsZ Rings in a Pleomorphic Chloroplast in Embryonic Cap Cells of Pelargonium zonale"Cytologia. 66,No.2. 227-233 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Higashiyama, T., et al.: "Pollen tube attraction by the synergid cell"Science. 293. 1480-1483 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kuroiwa, H., et al.: "Chloroplast division machinery as revealed by immunofluorescence and electron microscopy"Planta. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Kuroiwa, H., et al.: "Pelargonium embryogenesis : Cytological investigations of organelles in the early embrogenesis from the egg to the two-celled embryo"Sexual Plant Reproduction. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 黒岩晴子: "朝倉植物生理学講座第4巻「成長と分化-受粉と受精」(福田裕穂編)"朝倉書店. 11 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suzuki,L. et al.: "A zygote-specific protein with hydroxyproline-rich glycoprotein domains and lectin-like domains involved in the assembry of the cell wall of Chlamidomonous re."J.Phycol.. 36. 571-583 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Saito,C., et al.: "Unequal distribution of DNA-containing organelles in generative and sperm cells of Erythrina crista-galli (Fabaceae)"Sexual Plant Reproduction. 12. 296-301 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nagata,N. et al: "Unique positioning of mitochondria in developing microspores and pollen grains in Pharbitis nil : mitochondria cover the nuclear surface at specific developmental stages"Protoplasma. 213. 74-82 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kuroiwa T., et al: "Inheritance of cytoplasmic traits-embryological perspectives"Kluwer Academic Publisher. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Inada,N., et al.: "Senescence in the nongreening region of the rice (Oriza sativa) coleoptile"Protoplasma. 214. 180-193 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 黒岩晴子: "朝倉植物生理学講座第4巻「成長と分化-受粉と受精」(福田裕穂 編)"朝倉書店(刊行予定).

    • Related Report
      2000 Annual Research Report
  • [Publications] 黒岩晴子: "ハンドブック生物学データ集「植物の発生・成長・分化-発生」"朝倉書店(刊行予定).

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi