| Project/Area Number |
12640656
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物形態・構造
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| Research Institution | NISHIKYUSHU UNIVERSITY |
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Principal Investigator |
TAKAHASHI Tadao NISHIKYUSHU UNIVERSITY, FACULTY OF HEALTH AND WELFARE SCIENCE, PROFESSOR, 健康福祉学部, 教授 (90116616)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Paramecium / Microsurgical Technique / Cortical structure / Morphogenesis / Cortical unit / Stem units / Positional information / Silver impregnation / 走査電子顕微鏡 / SEMpore法 |
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| Research Abstract |
It is well known that the cell surface of the ciliate Paramecium consists of cortical units which are arranged in a certain regular pattern. It was previously considered that the organizing center of the cortical structures might exist in the center of the ventral equatorial region. In contrast, Takahashi et al. (1998) reported that in P. trichium the special cortical units, which are located in the most posterior position of 1C-2BB (two basal bodies with one cilium) units region, might contribute continuously to cortical reproduction ; that is, they may be the stem units. The purpose of this study was to examine this hypothesis using a microsurgical technique. We transected and removed the posterior 1/4 region of the cell, which probably included the hypothetical stem unit region, by using a fine glass needle, and then traced their regeneration processes during the three cell generations. We found that the missing part of the cell was gradually regenerated and completely recovered until the completion of the third cell division after the operation. These observations suggest that the formation of the whole cell surface structures is accomplished according to the positional information which is assumed to exist along the longitudinal cell axis, although the stem units may continuously supply new cortical units.
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