| Project/Area Number |
12650788
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物・生体工学
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
SHINOHARA Hiroaki Okayama University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (60178887)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Bioactive Peptides / Nonnatural amino acid / Photoisomerization / RGD peptide / Cell adhesion inhibition / Bradykinin / Cellular function control / Intelligent molecular devise / 細胞内カルシウム / 光刺激 / インテリジェントペプチド / 外部刺激 / ペプチドホルモン / PC12細胞 / ボオチン / アビジン |
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| Research Abstract |
The purpose of this study is to design, synthesize and characterize the photoresponsible nonnatural bioactive peptides. RGD (Arg-Gly-Asp) peptides and bradykinin analogues in which a photoisomerizable nonnatural amino acid, L-p-(phenylazo)phenylalanine (azoAla) was incorporated were designed and synthesized by solid phase synthesis. Reversible photoisomerization of the azoAla side chain in the peptides by alternative irradiation of UV and visible light was demonstrated by spectroscopic measurement. HPLC analysis indicated that the trans to cis isomerization of the azoAla side chain by UV irradiation induced the large decrease of hydrophobicity of the peptides. Finally it was clearly demonstrated that the cyclic RGD-azoAla peptide and the azoAka8-bradykinin analogue showed different bioactivity for the target cells at the trans state and the cis state.
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