| Project/Area Number |
12650873
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
高分子合成
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| Research Institution | Teikyo University of Science and Technology |
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Principal Investigator |
URYU Toshiyuki Teikyo University of Science and Technology, Department of Environmental and Material Engineering, Professor, 理工学部, 教授 (80011005)
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| Co-Investigator(Kenkyū-buntansha) |
KATSURAYA Kaname Wayo Womens University, Associate Professor, 短期大学部, 助教授 (20251465)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | AIDS vaccine / Dendrimer-type vaccine / Cyclic peptide / Cellobiose derivative / Lysine dendrimer / Reductive animation / Antibody productivity / AIDS virus gp120 / デンドリマー型 / ウイルス部分ペプチド / 糖鎖ペプチドデンドリマー / エンベロープ糖タンパク質 / V3ループペプチド / 抗体 / オルニチンデンドリマー / セロビオース |
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| Research Abstract |
A dendrimer-type AIDS (acquired immunodeficiency syndrome) vaccine having a possibility of high anti-HIV (human immunodeficiency virus) activity was molecularly designed and synthesized. It is expected that a medium molecular weight dendrimer-type vaccine carrying highly antigenic HIV peptide sequence can produce a large quantity of antibodies. Lysine dendrimer third (G3) and fourth (G4) generations, and ornithine dendrimer third generation were used for core dendrimers. Two methods by which a peptide sequnece included in HIV gp120 was bound to the dendrimer under mild conditions were developed. In the first method, eight amino groups in lysine dendrimer G3 were reacted with lactose, maltose, and maltotriose by reductive amination using BH3 pyridine catalyst to produce at most 16 oligosaccharides-bound dendrimers. In the next dendrimer elongation, primary 6-OH groups in the oligosaccharide were reacted with such dibasic acids as adipic acid and suberic acid to give dibasic acid-oligosaccharide-lysine dendrimer three-layered dendrimers. A four-layered dendrimer was also preprared. In the second method, cellobiose-lysine dendrimer was synthesized in which reducing end of the cellobiose moiety was directed outward the dendrimer. To the reducing end, a HIV cyclic peptide was tried to bind by reductive amination to form AIDS vaccine. Structure and molecular weight of the products were determined by NMR and TOF-MS. A model compound for AIDS vaccine was obtained. Intermolecular interactions between polypeptide and polysaccharide were detected by NMR in order to analyze an action mechanism of biologically active macromolecules such as vaccine.
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