| Project/Area Number |
12660007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
TAHARA Makoto Okayama University, Agriculture, Professor, 農学部, 教授 (50274014)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Sweetpotato / Retrotransposon / cultivar decline / Ty-1 copia |
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| Research Abstract |
Phenotypie mutations in sweetpotato (Ipomoea batatas (L.) Lam.) cultivars are possibly caused by stress-induced transposition of a retrotransposon present in sweetpotato genome.
Long terminal repeat (LTR) sequences of three different Ty1-copia retrotransposons were isolated from sweetpotato genome by the thermal asymmetric interlaced (TAIL) PCR using specific TAIL PCR primers designed by the partial sequences of the reverse transcriptase (RTase) and ribonuclease H (RNaseH) region of a retrotransposon, previously isolated from sweetpotato genome.
The TAIL PCR and single primer PCR methods were examined to display different insertion positions of these LTRs in sweetpotato genome ; however, both methods were found unreliable.
To identify stress-activating retrotransposon sequences in sweetpotato genome, RNAs were isolated from callus tissue, which was induced from meristem culture, and 3'RACE (3' random amplification of cDNA ends) was performed using RTase sequence primers. cDNAs corresponding to the RNAs polyadenyiated at the RNaseH end were isolated. The retrotransposon with this sequence is possibly activated in callus and can be used to relate activation of the retrotransposons with mutations in sweetpotato..
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