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Growth and development of juice vesicles grown by in vitro culture in Citrus

Research Project

Project/Area Number 12660022
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionShizuoka University

Principal Investigator

TAKAGI Toshihiko  Shizuoka University Dep. of Agriculture, Professor, 農学部, 教授 (40026612)

Co-Investigator(Kenkyū-buntansha) MUKAI Hiroo  Shizuoka University Dep. of Agriculture, Assistant Professor, 農学部, 助教授 (70201803)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsIn vitro culture / Satsuma mandarin / Juice vesicle / Sugar accumulation / Acid content / Sugar metabolizing enzymes / Water stress / Nitrogen nutrition / カンキツ / 砂じょう培養 / 酸集積 / 水ポテンシャル / 窒素成分
Research Abstract

The in vitro culture of juice vesicles could aid in studies on the sugar and acid metabolism in citrus fruit. In this report, juice vesicle explants including some mesocarp tissue excised from young satsuma mandarin (Citrus unshiu Marc.) fruit, were cultured in vitro on MS solid medium under dark conditions at 25 C.
1. The juice vesicles grew expansively and turned orange. The juice vesicles accumulated sugar, and disintegrated acid similar to those of fruit grown in the field when cultured on the medium supplemented with 10 % sucrose and incubated for two months. Sugar accumulation occurred in the juice vesicle explants excised from fruitlet at 51 days after full bloom or later and cultured on the medium. Effect of sucrose, glucose and fructose on vesicle growth and sugar accumulation was determined. Fructose was not effective for vesicle growth.
2. Juice vesicle explants were cultured on medium with various sucrose concentrations and water potentials. There is a close positive relationship between sugar and acid accumulated by vesicles and sucrose content in the medium with or without adjusting water potential. When cultured under different water potentials on 10 % sucrose concentration, the juice vesicles grew less but accumulated more sugar and acid as the water potential decreased.
3. When juice vesicle explants were cultured on medium with various nitrogen levels, there is a negative relationship between sugar content of juice vesicles and nitrogen content in the medium, but a positive one between acid content of juice and nitrogen in the medium.
4. Sugar composition in vesicles cultured on various carbohydrates indicates sugar degradation and conversion occurred in vesicle explants. Activities of sugar metabolizing enzymes, SS, SPS and Invertase, were higher in cultured juice vesicles than those of fruit grown in the field, however, the seasonal changes of those enzyme activities in both fruits were similar

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Mukai, H. et al.: "Sugar accumulation by in vitro cultured juice vesicles of satsuma mandarin"J. Japan. Soc. Hort. Sci.. 69. 57-59 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 向井啓雄: "培地の糖濃度および水ポテンシャルが培養砂じょうの糖集積に及ぼす影響"園芸学会雑誌. 70. 238-243 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada, H. et al.: "Effect of explant age, growth regulators and carbohydrates on sugar accumulation in Citrus juice vesicles cultured in vitro"Scientia Horticulturae. 90. 109-119 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 高木敏彦: "ウンシュウミカン砂じょうのIn vitro培養における培養時期および培養方法の検討"園芸学会雑誌. 69(別1). 73 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 原田久: "In vitroにおけるウンシュウミカン砂じょうの肥大・糖集積に及ぼす培地無機成分の影響"園芸学会雑誌. 69(別1). 74 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 向井啓雄: "In vitro培養におけるウンシュウミカン砂じょうの糖含量と糖代謝酵素活性の時期的変化"園芸学会雑誌. 69(別1). 75 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 向井啓雄: "ウンシュウミカンの砂じょう培養における培養温度が糖・酸含量に及ぼす影響"園芸学会雑誌. 69(別2). 120 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 高木敏彦: "数種カンキツの樹上果実および培養砂じょうにおけるさん含量の経時的変化"園芸学会雑誌. 70(別1). 90 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 高木敏彦: "培地の糖濃度がウンシュウミカン培養砂じょうの糖・酸含量および糖代謝酵素に及ぼす影響"園芸学会雑誌. 70(別2). 101 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mukai, H. et al: "Sugar accumulation by in vitro cultured juice vesicles of satsuma mandarin"J. Japan. Soc. Hort. Sci.. 69. 57-59 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mukai, H. et al.: "Effects of sugar concentration and water potential in the medium on sugar accumultion by in vitro cultured juice vesicles of Satsuma mandarin."J. Japan. Soc. Hort. Sci.. 70. 238-243 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada, H. et al.: "Effect of explant age, growth regulators and carbohydrates on sugar accumulation in Citrus juice vesicles cultured in vitro"Scientia Horticulturae. 90. 109-119 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takagi, T. et al.: "Times and method of in vitro culture of satsuma mandarin vesiles"J. Japan. Soc. Hort. Sci.. 69 (Suppl.1). 73 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada, H. et al.: "Effects of mineral elements on growth and sugar accumulation of in vitro ultured vesicles of Satsuma mandarin"J. Japan. Soc. Hort. Sci.. 69 (Suppl.1). 74 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mukai, H. et al.: "Seasonal changes of sugar content and sugar metabolizing enzyme activities of in vitro cultured vesicles of Satsuma mandarin"J. Japan. Soc. Hort. Sci.. 69 (Suppl.1). 75 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mukai, H. et al.: "Effect of temperature on sugar and acid contents of juice vesicle cultured in vitro"J. Japan. Soc. Hort. Sci.. 69 (Suppl.2). 120 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takagi, T. et al.: "Seasonal changes of acid of citrus juice vesicles on the tree and in vitro culture"J. Japan. Soc. Hort. Sci.. 70 (Suppl.1). 90 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takagi, T. et al.: "Effect of sugar concentration in medium on sugar and acid contents and activities of sugar metabolic enzymes of juice vesicles cultured in vitro in Satsuma mandarin"J. Japan. Soc. Hort. Sci. 70 (Suppl.2). 101 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 向井 啓雄 ら: "培地の糖濃度および水ポテンシャルが培養砂じょうの糖集積に及ぼす影響"園芸学会雑誌. 70. 238-243 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] HARADA, HISHASHI: "Effects of explant age, growth regulators and carbohydrates on sugar accumulation in Citrus juice vesicles cultured in vitro"Scientia Horticulturae. 90. 109-119 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 高木 敏彦 ら: "数種カンキツの樹上果実および培養砂じょうにおける酸含量の経時的変化"園芸学会雑誌. 70(別1). 90 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 高木 敏彦 ら: "培地の糖濃度がウンシュウミカン培養砂じょうの糖・酸含量および糖代謝酵素に及ぼす影響"園芸学会雑誌. 70(別2). 101 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] MUKAI HIROO: "Sugar accumulation by In vitro cultured juice vesicles of Satsuma mandarin"J.Japan.Soc.Hort.Sci.. 69. 57-59 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 高木敏彦: "ウンシュウミカン砂じょうのIn vitro培養における培養時期および培養方法の検討"園芸学会雑誌. 69(別1). 73 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 原田久: "In vitroにおけるウンシュウミカン砂じょうの肥大・糖集積に及ぼす培地無機成分の影響"園芸学会雑誌. 69(別1). 74 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 向井啓雄: "In vitro培養におけるウンシュウミカン砂じょうの糖含量と糖代謝酵素活性の時期的変化"園芸学会雑誌. 69(別1). 75 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 向井啓雄: "ウンシュウミカンの砂じょう培養における培養温度が糖・酸含量に及ぼす影響"園芸学会雑誌. 69(別2). 120 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 高木敏彦: "数種カンキツの樹上果実および培養砂じょうにおける酸含量の経時的変化"園芸学会雑誌. 70(別1). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 向井啓雄: "培地の糖濃度および水ポテンシャルがウンシュウミカン培養砂じょうの糖集積に及ぼす影響"園芸学会雑誌. 70(2). 238-243 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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