| Project/Area Number |
12660037
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
MASUTA Chikara Hokkaido Univ., Graduate School of Agriculture, Professor, 大学院・農学研究科, 教授 (60281854)
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| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Pland virus / Soybean stunt virus / Resistance / ダイズ萎縮 / SSV |
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| Research Abstract |
Host resistance to plant viruses is specifically observed depending on host-virus combination. Using Cucumber mosaic virus(CMV) and its soybean starain (SSV), we analyzed the resistant phenotype that a virus does not systemicaily spread but it it is localized in the inoculated leaves in two host-virus combinations (host species-CMVs and soybean cultivars-SSVs). In the latter combination, we determined where the resistance mechanism operates and suggested existence of two complementary dominant host factors to control the sobybean cultivar resistance to SSV. Both in cucumber-CMV and in soybean-SSV combinations, the primary determinant for systemic infection is the domain including the 3a gene and depending on cultivars, an additional viral gene (the CP gene) was required in the cultivar resistance to SSV, the S region of RNA3 (mainly the 5' non-translated region) and the region including the entire 2b gene were together necessary for the systemic movement. It is therefore consistant with the observation that there are two host factors to affect the viral sysmtemic infection.
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