• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The experimental verification of viroid-host adaptation

Research Project

Project/Area Number 12660038
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionHirosaki University

Principal Investigator

SANO Teruo  Hirosaki University, Faculty of Agriculture and Life Science, Associate Professor, 農学生命科学部, 助教授 (30142699)

Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsviroid / host adaptation / DNA shutting / random mutation / dominant selection / DNAシャッフリング / 宿主適応変異
Research Abstract

All pathogen has host specificity. Viroid, the smallest known pathogen, is an excellent model to analyze which part of the RNA molecule is responsible for host specificity. Hop stunt viroid (HSVd) spreads widely among various crops, and the unique sequence variations are found depending on the host species. To verify experimentally how unique variants has adapted to the varieties of hosts, (1) we have constructed random HSVd-variants consisting of various mutations found in the nature, inoculated them to several hosts, and analyzed how they adapt to each hosts, and (2) we have infected hops with natural HSVd isolates, cultivated them for 10 years, and examined the progeny sequences to analyze how each isolates adapted to hop.
The first, using random cDNA shuffling, we constructed random HSVd-variants. They were inoculated to cucumber, grapevine, peach, citrus, hop; the host for HSVd, and A. thaliana; non-host for HSVd. HSVd progeny accumulated in cucumber, grapevine, peach, citrus and hop, but no in A. thaliana. The nucleotide sequence analysis of progeny propagated in each species revealed that only HSVd-grapevine type was adaptable for grapevine but several types of chimeras between HSVd-grapevine and -citrus, as well as HSVd-grapevine type, were dominant in the other hosts. The variations characteristic for HSVd-plum were detected in hop and peach in the early stage of infection, but most of them disappeared in the later stage.
The second, hops were infected with four natural HSVd-isolates (HSVd-grapevine, -hop, -plum and -citrus) and cultivated for 10 years. All isolates were extracted every autumn for sequencing to analyze the rate of sequence variation. The rate of variation after the 10 years of persistent infection was the highest in HSVd-citrus and -plum, but significantly lower in HSVd-hop and -grapevine. The result indicated that the fitness of the four isolates to hop was different.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] SANO, T. et al.: "Phylogenetic analysis of hop and grapevine isolates of hop stunt viroid support a grapevine origin for hop stunt disease"Virus Genes. 21(1). 53-59 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Teruo Sano(分担執筆): "Viroids Hadidi, A. et al. eds. Biotechnological approaches for controlling viroid diseases 他4節"CISRO Publishing. 392 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] SANO, T.: "Phylogenetic analysis of hop and grapevine isolates of hop stunt viroid support a grapevine origin for hop stunt disease"Girus Genes. 21(1). 53-59 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] SANO, T., Hadidi, A. et al. eds.: "Hop stunt viroid in cucumber. In Viroids"CISRO Publishing. 134-136 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] SANO, T., Hadidi, A. et al. eds.: "Hop stunt viroid in plum and peach. In Viroids"CISRO Publishing. 165-167 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] SANO, T., Hadidi, A. et al. eds.: "Hop stunt viroids. In Viroids"CISRO Publishing. 207-212 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] SANO, T., Hadidi, A. et al. eds.: "Viroids in Japan. In Viroids"CISRO Publishing. 286-289 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] SANO, T., Hadidi, A. et al. eds.: "Biotechnological approaches for controlling viroid diseases. In Viroids"CISRO Publishing. 347-349 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Teruo Sano: "Viroids Hadidi. A. et al. eds. Biotechnological approaches for controlling viroid deseases他4節分担"CISRO Publishing(分担執筆).

    • Related Report
      2002 Annual Research Report
  • [Publications] SANO,T.,MIMURA.R.,OHSHIMA,K.: "Phylogenetic analysis of hop and grapevine isolates of hop stunt viroid support a grapevine origin for hop stunt disease"Virus Genes. 21巻1号. 53-59 (2001)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi