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Roles of chitin synthases in hyphal growth and differentiation of filamentous fungi

Research Project

Project/Area Number 12660066
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

HORIUCHI Hiroyuki  Graduate School of Agriculture and Life Sciences, The University of Tokyo, Associate Professor, 大学院・農学生命科学研究科, 助教授 (00209280)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordschitin synthase / tip growth / Aspergillus / conidiation / cell wall / 糸状菌
Research Abstract

Chitin is a homopolymer of β 1, 4-linked N-acetylglucosamine (GlcNAc) and one of the major cell wall component of many filamentous fungi. We have cloned five genes encoding chitin synthases (chsA - chsD, csmA) from Aspergillus nidulans so far. To elucidate the functional relationships among chsA - chsD, we constructed some sets of double mutants of these genes and analyzed their characteristics in this project. First, we investigate the cell wall organization of chsA chsC double mutant in detail by transmission electron microscopy. The cell walls and septa of the mutant were thickening and central pores of the septa were expanded. These defects were not observed in each single mutant. Chitin content of the cell walls of chsA chsC double mutant increased compared to those of each single mutant and the wild-type strain when they were grown on solid minimal medium. These results suggest that the cell wall defects caused by chsA chsC mutation activated other chitin synthase (s).
Next, we constructed chsB chsD, chsA chsB and chsC chsB double mutants and characterized them. The results showed that the importance of chsD increased for hyphal growth under the high osmolarity conditions when the expression of chsB was repressed and that chsA and chsC have some different roles in hyphal growth.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Ichinomiya et al.: "Repression of chsB expression reveals the functional importance of class IV chitin synthase gene chsD in hyphal growth and conidiation of Aspergillus nidulans"Microbiology. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masayuki Ichinoiya, Takayuki Motoyama, Makoto Fujiwara, Masamichi Takagi, Hiroyuki Horiuchi, and Akinori Ohta: "Repression of chsB expression reveals the functional importance of class IV chitin IV chitin synthase gene chsD in hyphal growth and conidiation of Aspergillus nidulans"Microbiology. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ichinomiya, et al.: "Repression of chsB expression reveals the functional importance of class IV chitin synthase gene chsD in hyphal growth and conidiation of Aspergillus nidulans"Microbiology. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fujiwara et al.: "Evidence that the Aspergillus nidulans class I and II chitin synthase genes, chsC and chsA, share critical roles in hyphal wall integrity and conidiophore development"Journal of Biochemistry. 127. 359-366 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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