| Project/Area Number |
12660106
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食品科学・栄養科学
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| Research Institution | Tohoku University |
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Principal Investigator |
YOSHIKI Yumiko Tohoku University, Graduate School of Life Sciences, Assistant, 大学院・生命科学研究科, 助手 (50311551)
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| Co-Investigator(Kenkyū-buntansha) |
OKUBO Kazuyoshi Tohoku University, Graduate School of Life Sciences, Prof., 大学院・生命科学研究科, 教授 (00005612)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | reactive oxygen / chemiluminescence / reactive oxygen scavenging activity / photon |
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| Research Abstract |
Photon emission was detected to study the distribution of reactive oxygen species (ROS), hydrogen donor and mediator on biological materials. Hydrogen donor and mediator emissions were observed from healthy person's serum at 250-260 cd/m^2. When healthy serum was standard emission line, serum from ROS related disease (cancer, allergy and diabetes) showed lower hydrogen donor emission and higher mediator emission. Self-immunity disease showed ROS-hydrogen donor emission and ROS-mediator emission. These results suggested that ROS scavenging emission system was useful for clinical and medical examinations. During disease, drawing blood gave strong stress to patients. In this standpoint, we applied this emission method to whole blood analysis and developed more sensitive detection method by several drops of blood with paper filter. Albumin from serum and catechins from tea and banana were identified by Photon-HPLC system based on ROS scavenging emission system. Photon-HPLC analysis of tea and spices suggested that ROS emission substance in food corresponded with hydrogen donor emission substance. ROS emission from wine increased depending on storage period and temperature. ROS emission from deterioration of wine showed the generation of ROS equivalent with 2.44 (opening red wine), 2.76 (heating red wine) and 2.18 mM H_2O_2 (heating white wine). A correlation of emission increase between taste study and ROS emission from deterioration wine indicated that our chemiluminescence method was serviceable for process, quality and storage evaluation for wine. Photon emission of healthy serum was detected after banana feeding which showed strongest hydrogen donor emission in food. Hydrogen donor emission from serum increased at 1-3 hour after feeding, these evidences supported that the combination of food for prevention disease and medical treatment.
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