| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Research Abstract |
Preventive effects of polyphenols, especially of flavonoids which a kind of polyphenols, against diseases were investigated by many researchers. However, physiological functions of anthocyanins, which is a kind of polyphenols and contained in fruits and vegetables with red - purple color peels, have not yet been fully investigated. In this project, the physiological functions of anthocyanins in vivo and function mechanism were investigated.
Major three acylated anthocyanins contained in red cabbage suppressed in vivo oxidation induced by paraquat which generate active oxygen species in vivo. Suppression of liver NADPH-cytochrome P450 reductase by feeding the acylated anthocyanins suggested that these anthocyanins partly could prevent oxidative stress by inhibiting the activity of this enzyme. On the other hands, Increases in lipid peroxidation of blood and liver, liver oxidized glutathione and 8-hydroxydeoxyguanosine (8-OH dG) generated with DNA damage, and suppression for release of α-tocopherol from the liver to blood by streptozotocin-induced diabetic rats were relieved in rats fed these anthocyanins, indicating that these anthocyanins were effective to prevent oxidative stress.
Anthocyanins contained in boysenberry , and Nasunin in egg plant suppressed increases in plasma GOT and GPT activities and release of α-tocopherol from liver to blood, increase in liver 8-OH dG, and oxidation of the liver ascorbic acid when those anthocyanins were fed to rats before induction of liver injury by the intraperitoneal injection of D-galactosamine. Stronger resistance of liver homogenate of rats fed anthocyanins before the injection of D-galactosamine, suggested that these anthocyanins or their oxidation products, which might be absorbed from intestine, partly acted as radical scavengers.
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