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Physiological significance of taurine chloramine induced inhibition of NF-_KB activation

Research Project

Project/Area Number 12660112
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionThe University of Tokyo

Principal Investigator

MIYAMOTO Yusei  The Univ. of Tokyo, Dept of Integrated Biosciences, Professor, 大学院・新領域創成科学研究科, 教授 (60157691)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordstaurine / taurine chloramine / neutrophill / NF-_KB / I_KB / oxidation of methionine residue / sulfoxide / anti-inflammatation / NF-кB / IкB / 蛋白の塩素化 / アポトーシス促進
Research Abstract

When this grant application was submitted, preliminary data showed modification of α-isoform of inhibitory protein (I_KB_α) by cell treatment with taurine chloramine (TauCl) that was judged by band shift observed in Western blots, suppression of I_KB_α degradation in response to stimulation and subsequent inhibition of nuclear factor _KB (NF_KB) activation. This suppression of I_KB_α degradation seemed to be due to its chlorination by TauCl. Therefore, this research was started with seeking amino acid residues of I_KB_α that are chlorinated by TauCl. Because the amino acid residue that is most easily chlorinated is tyrosine, tyrosine residues (eight tyrosine residues in I_KB_α) were mutated to alanine one by one. However, a mutant whose tyrosine residues were all converted to alanine showed band shift by TauCl treatment, indicated that tyrosine residue is not the chlorination site. Similar experiments were performed in regard to tryptophan. However, tryptophan was not a chlorination site, either. Then, amino acid residues modified by TauCl were further searched with deletion mutants of I_KB_α. As a result, the 45th methionine was found to be a site susceptible to TauCl modification. This methionine modification was estimated to be oxidation to form methionine sulfoxide by analysis of an oligo-peptide containing the 45th methionine with high performance liquid chromatography and mass spectrometry. These results lead to a unique hypothesis that protein oxidation at methionine residues may play a part in the cellular signal transduction.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 宮本有正: "好中球におけるタウリンのNFκB活性化阻害機構の生化学的解明"日本農芸化学会誌. 75・9. 980-984 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 宮本有正: "ラビット角膜上皮細胞における上皮成長因子によるNa : K:2Cl共輸送体活性上昇に関わる細胞シグナル系"Journal Membrane Biology. 183・2. 93-101 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 宮本有正: "組替えバキュロバイラスを用いて昆虫細胞に発現させたタウリン輸送体の特性解析"Protein Expression and Purification. 23・3. 389-397 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kanayama A, Shimizu M, and Miyamoto Y: "Biochemical analysis of taurine choloramine induced inhibition of NF_KB activation in neutrophils"Nippon Nougeikagaku Kaishi. 75 (9). 980-984 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yang H, Wang Y, Miyamoto Y, and Reinach, P. S: "Cell signaling pathways mediating epidermal growth factor stimulation of Na : K : Cl contransporter activity in rabbit corneal epithelial cells"Journal of Membrane Biology. 183 (2). 93-101 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyasaka T, Kaminogawa S, Shimizu M, Hisatsune T, Reinach P. S, and Miyamoto Y: "Characterization of human taurine transporter expressed in insect cells using a recombinant beculovirus"Protein Expression and Purification. 23 (3). 389-397 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 宮本有正: "好中球におけるタウリンのNFκB活性化阻害機構の生化学的解明"日本農芸化学会誌. 75・9. 980-984 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 宮本有正: "ラピッド角膜上皮細胞における上皮成長因子によるNa : K : 2Cl共輸送体活性上昇に関わる細胞シグナル系"Journal Membrane Biology. 183・2. 93-101 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 宮本有正: "組替えバキュロバイラスを用いて昆虫細胞に発現させたタウリン輸送体の特性解析"Protein Expression and Purification. 23・3. 389-397 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 宮本有正: "腸管上皮細胞におけるアミノ酸トランスポーターのインターフェロンYによる調節"必須アミノ酸研究. 158. 6-13 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 宮本有正: "小腸上皮細胞のナトリウム依存性グルコース輸送体に対する緑茶ポリフェノール類の競合阻害"Journal of Agricultural and Food Chemistry. 48・11. 5618-5623 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 宮本有正: "Caco-2細胞培養時に観察されるジサッカリダーゼ活性の上昇に対する酢酸による抑制効果"Journal of Nutrition. 130. 507-513 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 宮本有正: "胡麻中のタウリントランスポーター阻害物質の同定"Biosci.Biotechnol.Biochem.. 64・6. 1166-1172 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 宮本有正: "緑茶カテキン類による小腸グルコーストランスポーターの制御"Bio Factors. 13. 61-65 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 宮本有正: "Caco-2小腸上皮細胞単層のインターフェロンYによる過剰透過性にはiNOSの誘導やNOガスの発生は関与しない"Biosci.Biotechnol.Biochem.. (出版予定).

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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