| Project/Area Number |
12660138
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林学
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| Research Institution | Shimane University |
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Principal Investigator |
KAWAGUCHI Hideyuki Shimane University, Faculty of Life and Environmental Science, Associate Professor, 生物資源科学部, 助教授 (40202030)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Population / Aesculus turbinata / Reproduction / Pollen flow / Genetic structure / Seed dispersal / Seed production / Microsatellite / 豊凶 / 開花周期 / 結実周期 / 年変動 / 種子サイズ / 二次散布 / 動物散布 / 実生 / 遺伝子流 / 送粉 / 花粉親 / アロケーション |
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| Research Abstract |
The Japanese horse chestnut, Aesculus turbinata, is a tall deciduous tree species growing in cool temperate riparian forests in Japan. A. turbinata is andromonoecious and pollinated by mainly bumblebees. A. turbinata has large seeds which are dispersed by small mammals. A. turbinata occurs in riparian terrace and the lower part of slopes and often shows a linear-shaped distribution along a stream. Pollen flow, secondary seed dispersal, reproductive efforts, and genetic structure were analyzed in a linear-shaped population of A. turbinata along an upper narrow part of a stream. Six microsatellite makers were used for genetic analyses. Reproductive individuals of A. turbinata occurred about 1000m along the stream were sampled and analyzed as potential pollen donors. Seeds were collected from several individuals and their pollen donors were determined by microsatelite analyses. The number of pollen flow significantly decreased with increasing pollination distance. The amount of flowers and the timing of flowering in pollen donors were less effective on the number of pollen flow than the pollination distance. Fallen seeds were dispersed around mother tree by small mammals. Genetic similarity calculated from microsatellite analyses significantly decreased with increasing distance between individuals. The distance-dependent pollination and secondary seed dispersal around a mother tree may result in the spatial genetic structure in the A. turbinata population along the stream.
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