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Gene expression on fructification of edible mushroom

Research Project

Project/Area Number 12660153
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

OHGA Shoji  Faculty of Agriculture, Ass. Prof., 大学院・農学研究院, 助教授 (60117075)

Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsmushroom / enzyme / gene / shiitake / matsutake / laccase / cellulase / fructification / 水分 / 子実体 / ヒラタケ / ヒマラヤヒラタケ / 食用キノコ / 薬用キノコ / シュロガヤツリ / 多年生草本植 / 担子菌 / マイタケ / 菌床栽培
Research Abstract

Transcription of laccase and cellulase of Lentinula edodes was examined during fruit body development under different temperatures and moisture conditions on a sawdust-based substrate. Laccase transcript was maximal during the mycelial growth stage, and then declined rapidly at the fruiting stage. In contrast, cellulase transcript peaked at the veil-break stage during fruit body development. Transcriptions of these genes were triggered by microclimatic changes, specifically low temperature and osmotic pressure.
Effect of electric impulse was investigated on fruit body formation of Lentinula edodes in the sawdust-based substrate. Electric impulse of 15 kV was directly charged to the substrate at 3 different fruiting phases, 90-, 130- and 170-day cultures, respectively. Ergosterol contents and water conditions of the substrate were examined at the 3 phases. The electric impulse was effective to fruit body formation of 4 strains in 3 different fruiting types, which were for year-round cult … More ivation, for cultivation in spring and autumn, and for cultivation in winter. The effect of the electric impulse was more prominent as culture age proceeds.
Effect of electric impulse was investigated on fruit body formation of Lentinula edodes in the sawdust-based substrate. Electric impulse of 15 kV was directly charged to the substrate at 3 different fruiting phases, 90-, 130- and 170-day cultures, respectively. Ergosterol contents and water conditions of the substrate were examined at the 3 phases. The electric impulse was effective to fruit body formation of 4 strains in 3 different fruiting types, which were for year-round cultivation, for cultivation in spring and autumn, and for cultivation in winter. The effect of the electric impulse was more prominent as culture age proceeds.
Ribonucleic acid (RNA-M) and 5' nucleotide mixture (RNA-Nt) were added to the mycelial colony (shiro) of Tricholoma matsutake prior to fruiting stage. Forty g ribonucleic acid derivatives was dissolved in 4,000 ml distilled water, and sprayed directly to 4 m^2 surface ground of the pine forest. Glucosamine ergosterol and phospholipid were useful indicators to determine the fungal biomass in the fine roots. These indicator components in the fine roots increased after RNA-M and RNA-Nt addition, and then revealed obviously higher levels compare with non-addition control plots. Addition of these compounds resulted to mycelial biomass increment judging from 3 indicator responses. Fruit body formation occurred around 30 days after addition of RNA-M and RNA-Nt. Total amount of fruit body was 230 g (9 fruit bodies) (RNA-M) and 210 g (7 fruit bodies) (RNA-Nt) during 14 days observation period. In contrast, yield of control plots were 0-60 g (0-2 fruit bodies) in the experimental area. Yield and number of T. matsutake resulted increment with the addition of nucleic acid related compounds. Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Ohga, S., Royse, D.J.: "Transcriptional regulation of laccase and cellulase genes during growth and fruiting of Lentinula edodes on supplemented sawdust"FEMS Microbiol.Lett.. 201. 111-115 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lee, S-S., Ohga, S.: "Several genes expression on fructification of Lentinula edodes"J.Fac.Agr., Kyushu Univ.. 46. 47-59 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohga, S., Iida, S., Koo, C-D., Cho, N-S.: "Effect of electric impulse on fruit body production of Lentinula edodes in the sawdust-based substrate"Mushroom Sci.Biotechnol.. 9. 7-12 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 大賀祥治, 具 昌徳, 金 載水, 趙 南ソク, 眞許勝弘, 寺下隆夫, 森永 力, 堀越孝雄, 江口文陽, 北本 豊: "マツタケ子実体発生におよぼす核酸関連物質の効果"九大演報. 83. 43-52 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohga, S., Royse, D.J.: "Transcriptional regulation of laccase and cellulase genes during growth and fruiting of Lentinula edodes on supplemented sawdust"FEMS Microbiol. Lett.. 201. 111-115 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lee, S-S., Ohga, S.: "Several genes expression on fructification of Lentinula edodes"J. Fac. Agr. Kyushu Univ.. 46. 47-59 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohga, S., Iida, S., Koo, C-D., Cho, N-S.: "Effect of electric impulse on fruit body production of Lentinula edodes in the sawdust-based substrate"Mushroom Sci. Biotechnol.. 9. 7-12 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohga, S., Koo, C.-D., Kim, J.-S., Cho, N.-S., Mamoto, K., Terashima, T., Morinaga, T., Horikoshi, T., Eguchi, F., Kitamoto, Y.: "Utilization of nucleic acid compounds for fruiting of Tricholoma matsutake"Bull. Kyushu Univ. For.. 83. 43-52 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohga, S., Royse, D.J.: "Transcriptional regulation of laccase and cellulase genes during growth and fruiting of Lentinula edodes on supplemented sawdust"FEMS Microbiol. Lett.. 201. 111-115 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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