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Functional analysis of the classical complement pathway in bony fish

Research Project

Project/Area Number 12660173
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

NAKAO Miki  Faculty of Agriculture, KYUSHU UNIVERSITY, Assistant Professor, 大学院・農学研究院, 助手 (50212080)

Co-Investigator(Kenkyū-buntansha) YANO Tomoki  Faculty of Agriculture, KYUSHU UNIVERSITY, Professor, 大学院・農学研究院, 教授 (90038266)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordscomplement / bony fish / carp / the classical pathway / molecular cloning / gene diversity / evolution / C1 / C4 / クローニング / 系統発生 / 生体防御
Research Abstract

The classical complement pathway of mammals is activated by antigen-bound antibody and plays a crucial role in elimination of invading pathgens. Although the presence of the classical pathway is evident in bony fish, its reaction mechanism remains to be elucidated. In the present study, components involved in the classical pathway activation of the common carp complement was identified by molecular cloning and functional analysis. Complementary DNA clones encoding two C1r/s isotypes, two C4 isotypes and a novel truncated isoform of the mannose-binding protein-associated serine protease (MASP) have been isolated from carp hepatopancreas CDNA library. These show a substantial sequence diversity in several functional sites, suggesting their functional difference. Furthermore, three B/C2-A isotypes were also cloned from the same library. They are synthesized in different tissues at different levels. In particular, a novel isotype, designated B/C2-A3, is expressed exclusively in the lymphoid organs such as kidney and spleen, and its expression is up-regulated, on beta-glucan-injection, suggesting that B/C2-A3 isotype is an acute-phase reactant. The functional divergence of the complement component isotypes implicates that the diversity is a unique evolutioary strategy taken by bony fish to expand its innate immune-recognition.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Nakao M, Matsumoto M, Nakazawa M, Fujiki K, Yano T: "Diversity of complement factor B/C2 in the common carp (Cyprinus carpio): Three isotypes of B/C2-A expressed in different tissues"Dev. Comp. Immunol.. 26(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakao M, Osaka, Fujiki K, Yano T.: "Molecular cloning of the complement C1r/C1s/MASP2-like serine proteases from the common carp (Cyprinus carpio)"Immunogenetics. 52. 255-263 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Bayne, C.J., Gerwick L., Fujiki, K., Nakao, M., Yano, T: "Immune relevant genes identified in the liver of rainbow trout, Oncorhynchus mykiss, by means of suppression subtractive"Dev. Comp. Immunol.. 25. 205-217 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujiki K, Bayne CJ, Shin DH, Nakao M, Yano T: "Molecular cloning of carp (Cyprinus carpio) C-type lectin and pentraxin by use of suppression subtractive hybridisation"Fish Shellfish Immunol.. 11. 275-279 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shin DH, Fujiki K, Nakao M, Yano T.: "Organization of the NKEF gene and its expression in the common carp (Cyprinus carpio)"Dev. Com. Immunol.. 25. 597-606 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakao M, Muturo J, Yano T: "Expansion of the genes encoding complement components in bony fish"Recent Research Developments in Immunology. 3. 15-32 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakao, M., Matsumoto, M., Nakazawa M., Fujiki K., Yano T.: "Diversity of complement factor B/C2 in the common carp (Cyprinus carpio): Three isotypes B/C2-A expressedin different tissues"Dev. Comp. Immunol.. 26 (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakao, M., Osaka, Fujiki K., Yano T.: "Molecular cloning of the complement C1r/C1s/MASP2-like serine proteases from the common carp (Cyprinus carpio)"Immunogenetics. 52. 255-263 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Bayne, C. J., Gerwick L., Fujiki K., Nakao, M. and Yano T.: "Immune relevant genes identified in the liver of rainbow trout, Oncorhynchus mykiss, by means of suppression subtractive hybridization"Dev. Comp. immunol.. 25. 205-217 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujiki K, Bayne CJ, Shin DH, Nakao M, Yano T.: "Molecular cloning of carp (Cyprinus carpio) C-type lectin and pentraxin by use of suppression subtracts hybridisation"Fish Shellfish Immunol.. 11. 275-279 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shin DH, Fujiki K, Nakao M. Yano T.: "Organization of the NKEF gene and its expression in the common carp (Cyprinus carpio)"Dev. Comp. immunol.. 25. 597-606 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakao M, Mutsuro J. and Yano T.: "Expansion of the genes encoding somplement components in bony fish"Recent Research Developments in Immunology. 3. 15-32 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakao M, Matsumoto M, Nakazawa M, Fujiki K, Yano T: "Diversity of complement factor B/C2 in the common carp (Cyprinus carpio): Three isotypes of B/C2-A expressed in different tissues"Dev. Comp. Immunol.. 26(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakao M, Osaka, Fujiki K, Yano T.: "Molecular cloning of the complement C1r/C1s/MASP2-like serine proteases from the common carp (Cyprinus carpio)"Immunogenetics. 52. 255-263 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Bayne, C.J., Gerwick L., Fujiki, K., Nakao, M., Yano, T: "Immune relevant genes identified in the liver of rainbow trout, Oncorhynchus mykiss, by means of suppression subtractive hybridization"Dev. Comp. Immunol.. 25. 205-217 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fujiki K, Bayne CJ, Shin DH, Nakao M, Yano T: "Molecular cloning of carp (Cyprinus carpio) C-type lectin and pentraxin by use of suppression subtractive hybridisation"Fish Shellfish Immunol.. 11. 275-279 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shin DH, Fujiki K, Nakao M, Yano T.: "Organization of the NKEF gene and its expression in the common carp (Cyprinus carpio)"Dev. Comp. Immunol.. 25. 597-606 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakao M, Mutsuro J, Yano T: "Expansion of the genes encoding complement components in bony fish"Recent Research Developments in Immunology. 3. 15-32 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M Nakao,J Mutsuro,R Obo,K Fujiki,M Nonaka,T Yano: "Molecular cloning and protein analysis of divergent forms of the complement component C3 from a bony fish, the common carp (Cyprinus carpio) : presence of variants lacking the catalytic histidine"European Journal of Immunology. 30. 858-866 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Nagai,J.Mutsuro,M.Kimura,Y.Kato,K.Fujiki,T.Yano,M.Nakao: "A novel truncated isoform of the mannose-binding lectin-associated serine protease (MASP) from the common carp (Cyprinus carpio)"Immunogenetics. 51. 193-200 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Muturo J,Nakao M,Fujiki K,Yano T: "Multiple forms of α2-macroglobulin from a bony fish, the common carp (Cyprinus carpio) : Striking sequence diversity in functional sites"Immunogenetics. 51. 847-855 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 中尾実樹,矢野友紀: "硬骨魚類の補体系-補体成分の多様性から特有の生体防御戦略を探る-"化学と生物. 38. 582-588 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakao M,Osaka K,Kato Y,Fujiki K,Yano T: "Molecular cloning of the complement C1r/C1s/MASP2-like serine proteases from the common carp (Cyprinus carpio)"Immunogenetics. 52. 255-263 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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