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Enzyme system responsible for the generation of superoxide anion in red tide plankton, Chattonella marina

Research Project

Project/Area Number 12660174
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionNagasaki University

Principal Investigator

ODA Tatsuya  Nagasaki University, Faculty of Fisheries, Professor, 水産学部, 教授 (60145307)

Co-Investigator(Kenkyū-buntansha) HATAKEYAMA Tomomitsu  Nagasaki University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (50228467)
ISHIMATSU Atsushi  Nagasaki University, Faculty of Fisheries, Professor, 水産学部, 教授 (00184565)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsRed Tide / Plankton / Reactive Oxygen Species / Toxicity / Chattonella / Raphyidophyceae / Lectin / グリコキャッリックス / NADPH oxidase
Research Abstract

Chattonella marina, a raphidophycean flagellate, is one of the most noxious red tide phytoplankton and is highly toxic to fish, especially to yellowtail Seriola quinqueradiata. Although the precise mechanism of the toxic action of Chattonella sp. remains unclear, it has been demonstrated that a decrease in oxygen partial pressure of arterial blood is the earliest physiological disturbance observed in fish after exposure" to C. marina. Physiological and histological studies of fish exposed to C.\marina also'suggested that;the blockade of respiratory water flow through the gill lamellae caused by excessive mucusiinterferes with O2 transfer, resulting in asphyxia. C. marina is known to produce reactive oxygen species (ROS) such as superoxide anion (O2-), hydrogen peroxide (H2O2), and hydroxyl radical (-OH) under normal physiological conditions. Although several lines of evidence suggest that ROS are involved in the mortality of fish exposed to C. marina, the mechanism; of ROS generation in C. marina is remained to be clarified. In this study, we found that the cell-free supernatant prepared from C. marina cells showed NAD(P)H-dependent O2- generation; and this response was inhibited by diphenyleneiodonium (DPI), a specific inhibitor of mammalian NADPH oxidase. When the cell-free supernatant of C. marina was analyzed by immunoblotting using antibody raised against the human neutrophil cytochrome b558 large subunit (91 kDa), a main band with approximately 110 kDa was detected. Furthermore, the cell surface localization of the epitope recognized with this antibody was demonstrated in C. marina by indirect immunofluorescence. These results provide evidence for the involvement of enzymatic system analogous to the neutrophil NADPH oxidase as a source of O2- production in C. marina.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (54 results)

All Other

All Publications (54 results)

  • [Publications] N.Sadakata, T.Oda, N.Komatsu, T.Muramatsu: "Effects of glutathione-related compounds on increased caspase-3 and caspase-6-like activities in ricin-treated U937 cells"Biosci.Biotechnol.Biochem.. 64・1. 202-205 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D.Kim, T.Oda, A.Ishimatsu, T.Muramatsu: "Galacturonic-acid-induced increase of superoxide production in red tide phytoplankton Chattonella marina and Heterosigma akashiwo"Biosci.Biotechnol.Biochem.. 64・4. 911-914 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Matsubara, R.Kawata, K.Iwasaki, Y.Kimura, T.Oda, T.Muramatsu: "Cloning and sequence analysis of a gene (aly PG) encoding poly(a-L-guluronate)lyase from Corynebacterium sp.strain ALY-1"J.Biosci.Bioengineering. 89・2. 199-202 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Oda, N.Komatsu, T.Muramatsu, A.Nonaka, Suminto, K.Hirayama: "Detoxifying ability of marine bacterium Flavobacterium sp.against a toxic contaminant in the reagent sodium chloride to Nannochloropsis oculata"Fisheries Science. 66・2. 241-248 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Kawamura, T.Oda, T.Muramatsu: "Purification and characterization of a dipeptidyl carboxypeptidase from the polychaete Neanthes virens resmbling angiotensin I converting enzyme"Com.Biochem.Pysiol.. B126. 29-37 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N.Hasegawa, Y.Kimura, T.Oda, N.Komatsu, T.Muramatsu: "Isolated ricin B-chain-mediated apoptosis in U937 cells"Biosci.Biotechnol.Biochem.. 64・7. 1422-1439 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Okamoto, D.Kim, T.Oda, K.Matsuoka, A.Ishimatsu, T.Muramatsu: "Concanavalin A-induced discharge of glycocalyx of raphidophycean flagellates, Chattonella marina and Heterosigma akashiwo"Biosci.Biotechnol.Biochem.. 64・8. 1767-1770 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N.Komatsuo, M.Nakagawa, T.Oda, T.Muramatsu: "Depletion of intracellular NAD+ and ATP levels during ricin-induced apoptosis through the specific ribosomal inactivation results in the cytolysis of U937 cells"J.Biochem.. 128・3. 463-470 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Kawamura, K.Kikuno, T.Oda, T.Muramatsu: "Some molecular and inhibitory specifications of a dipeptidyl carboxypeptidase from the polychaete Neanthes virens resembling angiotensin I converting enzyme"Biosci.Biotechnol.Biochem.. 64・10. 2193-2200 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D.Kim, Y.Sato, T.Oda, T.Muramatsu, Y.Matsuyama, T.Honjo: "Specific toxic effect of dinoflagellate Heterocapsa circularisquama on the rotifer Brachionus plicatilis"Biosci.Biotechnol.Biochem.. 64・12. 2719-2722 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D.Kim, A.Nakamura, T.Okamoto, N.Komatsu, T.Oda, T.Iida, A.Ishimatsu, T.Muramatsu: "Mechanism of superoxide anion generation in the toxic red tide phytoplankton Chattonella marina : possible involvement of NAD(P)H oxidase"Biochim.Biophysi.Acta. 1524・. 220-227 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Iwamoto, R.Araki, K.Iriyama, T.Oda, H.Fukuda, S.Hayashida, T.Muramatsu: "Purification and characterization of bifunctional alginate lyase from Alteromonas sp.strain No.272 and its action on saturated oligomeric substrates"Biosci.Biotechnol.Biochem.. 65・1. 133-142 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Oda, Y.Sato, D.Kim, T.Muramatsu, Y.Matsuyama, T.Honjo: "Hemolytic activity of Heterocapsa circularisquama (dinophyceae) and its possible involvement in shellfish toxicity"J.Phycol.. 37・. 509-516 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Miyazaki, J.Obata, Y.Iwamoto, T.Oda, T.Muramatsu: "Calcium-sensitive extracellular poly (a-L-guluronate) lyase from a marine bacterium Pseudomonas sp.strain F6 : Purification and some properties"Fisheries Sci.. 67・. 956-964 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D.Kim, T.Okamoto, T.Oda, K.Tachibana, K.S.Lee, A.Ishimatsu, Y.Matsuyama, T.Honjo, T.Muramatsu: "Possible involvement of the glycocalyx in the ichthyotoxicity of Chattonella marina (Raphidophyceae) : immunological approach using antiserum against cell surface structures of the flagellate"Mar.Biol.. 139・. 625-632 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N. Sadakata, T. Oda, N. Komatsu, and T. Muramatsu: "Effects of glutathione related compounds on increased caspase-3 and caspase-6-like activities in ricin-treated U937 cels"Biosci. Biotechnol. Biochem.. 64. 202-205 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D. Kim, T. Oda, A. Ishimatsu, and T. Muramatsu: "Galacturonic acid-induced increase of superoxide production in red tide phytoplankton Chattonella marina and Heterosigna akashiwo"Biosci. Biotechnol. Biochem.. 64. 911-914 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Matsubara, R. Kawada, K. Iwasaki, Y. Kimura, T. Oda, and T. Muramatsu: "Cloning and sequence analysis of gene (alyPG) encoding poly(a-guluronate)lyase from Corynebacterium sp. strain ALY-1"J. Biosci. Bioengineering. 89. 199-202 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Oda, N. Komatsu, T. Muramatsu, A. Nonaka, Suminto, and K. Hirayama: "Detoxifying ability of marine bacterium Flavobacterium sp. against a toxic contaminant in the reagent sodium chloride to Nannochloropsis oculata"Fisheries Science. 66. 241-248 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Kawamura, T. Oda, and T. Muramatsu: "Purification and characterization of a dipeptidyl carboxypeptidase from the polychaete Neanthes virens resembling angiotensin converting enzyme"Com. Biochem. Pysiol.. B126. 29-37 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N. Hasegawa, Y. Kimura, T. Oda, and T. Muramatsu: "Isolated ricin B-chain-mediated apoptosis in U937 cells"Biosci. Biotechnol. Biochem.. 64. 14221439 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Okamoto, D. Kim, T. Oda, K. Matsuoka, A. Ishimatsu, and T. Muramatsu: "Concanavalin A-induced discharge of glycocalyx of raphidophycean flagellates, Chattonella marina and Heterosigm a akashiwo"Biosci. Biotechnol. Biochem.. 64. 1767-1770 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N. Komatsu, M. Nakagawa, T. Oda, and T. Muramatsu: "Depletion of intracellular NAD+ and ATP levels during ricin-induced apoptosis through the specific ribosomal inactivation results in the cytolysis of U937 cells"J. Biochem.. 128. 463-470 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D. Kim, Y. Sato, T. Oda, T. Muramatsu, Y. Matsuyama, and T. Honjo: "Specific toxic effect of dinoflagellate Heterocapsa circularisquama on the rotifer Brachionus plicatilis"Biosci. Biotechnol. Biochem.. 64. 2719-2722 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Kawamura, K. Kikuno, T. Oda, and T. Muramatsu: "Some-molecular and inhibitory specifications of a dipeptidyl carboxypeptidase from the polychate Neanthes virens resembling angiotensin I converting enzyme"Biosci.l Biotechnol. Biochem.. 64. 2193-2200 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D. Kim, A. Nakamura, T. Okamoto, N. Komatsu, T. Oda, T. Iida, A. Ishimaysu, and T. Muramatsu: "Mechanism of superoxide anion generation in the toxic red tidephytoplankton Chattonella marina : possible involvement of NAD(P)H oxidase"Biochim. Biophysi. Acta. 1524. 220-227 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Iwamoto, R. Araki, K. Iriyama, T. Oda, H. Fukuda, S. Hayashida, and T. Muramatsu: "Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain No.272 and its action on saturated oligomeric substrates"Biosci. Biotechnol. Biochem.. 65. 133-142 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Oda, Y. Sato, D. Kim, T. Muramatsu, Y. Matsuyama, and T. Honjo: "Hemolytic activity of Heterocapsa circularisquama (Dinophyceae) and its possible involvement in shellfish toxicity"J. Phycol.. 37. 509-516 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Miyazaki, J. Obata, Y. Iwamoto, T. Oda, and T. Muramatsu: "Calcium-sensitive extracellular poly(a-L-guluronate)lyase from a marine bacterium Pseudomonas sp. strain F6 : Purification and some properties"Fisheries Science. 67. 956-964 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D. Kim, T. Okamoto, T. Oda, K. Tachibana, K.-S. Lee, A. Ishimatsu, Y. Matsuyama, T. Honjo, And T. Muramatsu: "Possible involvement of glycocalyx in the ichthyotoxic mechanism of Chattonella marina"Mar. Biol.. 139. 625-632 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N.Sadakata, T.Oda, N.Komatsu, T.Muramatsu: "Effects of glutathione-related compounds on incresased caspase-3 and caspase-6-like activities in ricin-treated U937 cells"Biosci.Biotechnol.Biochem.. 64・1. 202-205 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] D.Kim, T.Oda, A.Ishimatsu, T.Muramatsu: "Galacturonic-acid induced increase of superoxide production in red tide phytoplankton Chattonella marina and Heterosigma akashiwo"Biosci.Biotechnol.Biochem.. 64・4. 911-914 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Matsubara, R.Kawata, K.Iwasaki, Y.Kimura, T.Oda, T.Muramatsu: "Cloning and sequence analysis of a gene(aly PG)encoding poly(a-L-guluronate)Iyase from Corynebacterium sp. Strain ALY-1"J.Biosci.Bioengineering. 89・2. 199-202 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Oda, N.Komatsu, T.Muramatsu, A.Nonaka, Suminto, K.Hirayama: "Detoxifying ability of marine bacterium Flavobacterium sp. against a toxic contaminant in the reagent sodium chloride to Nannochloropsis oculata"Fisheries Science. 66・2. 241-248 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Kawamura, T.Oda, T.Muramatsu: "Purification and characterization of a dipeptidyl carboxypeptidase from the polychaete Neanthes virens resmbling angiotensin I converting enzyme"Com.Biochem.Pysiol.. B126. 29-37 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] N.Hasegawa, Y.Kimura, T.Oda, N.Komatsu, T.Muramatsu: "Isolated ricin B-chain-mediated apoptosis in U937 cells"Biosci.Biotechnol.Biochem.. 64・7. 1422-2000 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Okamoto, D.Kim, T.Oda, K.Matsuoka, A.Ishimatsu, T.Muramatsu: "Concanavalin A-induced discharge of glycocalyx of raphidophycean flagellates, Chattonella marina and Heterosigma akashiwo"Biosci.Biotechnol.Biochem.. 64・8. 1767-1770 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] N.Komatsuo, M.Nakagawa, T.Oda, T.Muramatsu: "Depletion of intracellular NAD+ and ATP levels during ricin-induced apoptosis through the specific ribosomal inactivation results in the cytolysis of U937 cells"J.Biochem.. 128・3. 463-470 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Kawamura, K.Kikuno, T.Oda, T.Muramatsu: "Some molecular and inhibitory specifications of a dipeptidyl carboxypeptidase from the polychaete Neanthes virens resembling angiotensin I converting enzyme"Biosci.Biotechnol.Biochem.. 64・10. 2193-22000 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] D.Kim, Y.Sato, T.Oda, T.Muramatsu, Y.Matsuyama, T.Honjo: "Specific toxic effect of dinoflagellate Heterocapsa circularisquama on the rotifer Brachionus plicatilis"Biosci.Biotechnol.Biochem.. 64・12. 2719-2722 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] D.Kim, A.Nakamura, T.Okamoto, N.Komatsu, T.Oda, T.Iida, A.Ishimatsu, T.Muramatsu: "Mechanism of superoxide anion generation in the toxic red tide phytoplankton Chattonella marina : possible involvement of NAD(P)H oxidase"Biochim.Biophysi.Acta. 1524. 330-227 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Iwamoto, R.Araki, K.Iriyama, T.Oda, H.Fukuda, S.Hayashida, T.Muramatsu: "Purification and characterization of bifunctional alginate lyase from Alteromonas sp.strain No.272 and its action on saturated oligomeric substrates"Biosci.Biotechnol.Biochem.. 65・1. 133-142 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Oda, Y.Sato, D.Kim, T.Muramatsu, Y.Matsuyama, T.Honjo: "Hemolytic activity of Heterocapsa circularisquama(dinophyceae) and its possible involvement in shellfish toxicity"J.Phycol.. 37. 509-516 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Miyazaki, J.Obata, Y.Iwamoto, T.Oda, T.Muramatsu: "Calcium-sensitive extracellular poly(a-L-guluronate)lyase from a marine bacterium Pseudomonas sp. strain F6 : Purification and some properties"Fisheries Sci.. 67. 956-964 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] D.Kim, T.Okamoto, T.Oda, K.Tachibana, K.S.Lee, A.Ishimatsu, Y.Matsuyama, T.Honjo, T.Muramatsu: "Possible involvement of the glycocalyx in the ichthyotoxicity of Chattonella marina(Raphidophyceae): immunological approach using antiserum against cell surface structures of the flagellate"Mar.Biol.. 139. 625-632 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] N.Sadakata,T.Oda,N.Komatsu,and T.Muramatsu: "Effects of glutathione-related compounds on increased caspase-3 and caspase-6-like activities in ricin-treated U937 cells"Biosci.Biotechnol.Biochem.. 64・1. 202-205 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] D.Kim,T.Oda,A.Ishimatsu,and T.Muramatsu: "Galacturonic-acid-induced increase of superoxide production in red tide phytoplankton Chattonella marina and Heterosigma akashiwo"Biosci.Biotechnol.Biochem.. 64・4. 911-914 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Matsubara,R.Kawata,K.Iwasaki,Y.Kimura,T.Oda,and T.Muramatsu: "Cloning and sequence analysis of a gene (aly PG) encoding poly(a-L-guluronate)lyase from Corynebacterium sp.strain ALY-1"J.Biosci.Bioengineering. 89・2. 199-202 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Oda,N.Komatsu,T.Muramatsu,A.Nonaka,Suminto,and K.Hirayama: "Detoxifying ability of marine bacterium Flavobacterium sp.against a toxic contaminant in the reagent sodium chloride to Nannochloropsis oculata"Fisheries Science. 66・2. 241-248 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Kawamura,T.Oda,,and T.Muramatsu: "Purification and characterization of a dipeptidyl carboxypeptidase from the polychaete Neanthesvirens resmbling angiotensin I converting enzyme"Com.Biochem.Pysiol.. B126. 29-37 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N.Hasegawa,Y.Kimura,T.Oda,N.Komatsu,and T.Muramatsu: "Isolated ricin B-chain-mediated apoptosis in U937 cells"Biosci.Biotechnol.Biochem.. 64・7. 1422-2000 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Okamoto,D.Kim,T.Oda,K.Matsuoka,A.Ishimatsu,and T.Muramatsu: "Concanavalin A-induced discharge of glycocalyx of raphidophycean flagellates, Chattonella marina and Heterosigma akashiwo"Biosci.Biotechnol.Biochem.. 64・8. 1767-1770 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N.Komatsuo,M.Nakagawa,T.Oda,and T.Muramatsu: "Depletion of intracellular NAD^+ and ATP levels during ricin-induced apoptosis through the specific ribosomal inactivation results in the cytolysis of U937 cells"J.Biochem.. 128・3. 463-470 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Kawamura,K.Kikuno,T.Oda,and T.Muramatsu: "Some molecular and inhibitory specifications of a dipeptidyl carboxypeptidase from the polychaete Neanthesvirens resembling angiotensin I converting enzyme"Biosci.Biotechnol.Biochem.. 64・10. 2193-2200 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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