| Project/Area Number |
12660183
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Tokyo University of Fisheries |
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Principal Investigator |
KIMURA Bon Department of Fisheries,Tokyo University of Fishereis, Associate Professor, 水産学部, 助教授 (50262340)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | Carbon dioxide / Clostridium botulinum / spore germination / food stored in chilled / crab / reduced heat sterilization / quantitative PCR / 低温管理 / 包装鮮魚 / 無菌米飯 / 胞子 / 発芽 |
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| Research Abstract |
The effect of CO2 on the germination of Clostriiudium botulinum under cold emperature such as 5℃ was studied. During the course of investigations into the effect of CO2 on the germination at 5℃, it was observed that spores of this organism lose heat stability when the spores were placed under CO2 atmospheres for short period of time. This investigation was undertaken to determine the effect of CO2 atmosphere on the heat inactivation of spore of C. botulinum on an agar surface and several foods at cold temperatures. It was demonstrated that the number of viable spore decreases significantly by mild heat treatment (60℃, 30min) when the sprores were incubated under CO2 atmosphere under cold temperatues (5℃). Also, in foods treated with reduced heat sterilization to make the taste much better, there is a risk of contamination of C. botulinum. It is difficult to prevent from preventing contamination of high heat tolerant score formers into these products during packaging. Thus, this study also aimed to assess risks in aseptic boiled rice with C.botulinum type A and B. Moreover, A rapid quantitative PCR assay (TaqMan assay) which quantifies C. botulinum type E by amplifying a 280-bp sequence from the botulinum neurotoxin type E (BONT/E)gene is developed. With this method, which uses the hydrolysis of an internal fluoregenic probe and monitors in real-time the increase in the intensity of fluorescence during PCR by using theABI PrismTM 7700 Sequence Detection System (SDS), it was possible to perform accurate and reproducible quantification of the C.botulinumtype E toxin gene.
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