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The effect of carbon dioxide on the germination and prolification of Clostridium botulinum

Research Project

Project/Area Number 12660183
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionTokyo University of Fisheries

Principal Investigator

KIMURA Bon  Department of Fisheries,Tokyo University of Fishereis, Associate Professor, 水産学部, 助教授 (50262340)

Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
KeywordsCarbon dioxide / Clostridium botulinum / spore germination / food stored in chilled / crab / reduced heat sterilization / quantitative PCR / 低温管理 / 包装鮮魚 / 無菌米飯 / 胞子 / 発芽
Research Abstract

The effect of CO2 on the germination of Clostriiudium botulinum under cold emperature such as 5℃ was studied. During the course of investigations into the effect of CO2 on the germination at 5℃, it was observed that spores of this organism lose heat stability when the spores were placed under CO2 atmospheres for short period of time. This investigation was undertaken to determine the effect of CO2 atmosphere on the heat inactivation of spore of C. botulinum on an agar surface and several foods at cold temperatures. It was demonstrated that the number of viable spore decreases significantly by mild heat treatment (60℃, 30min) when the sprores were incubated under CO2 atmosphere under cold temperatues (5℃). Also, in foods treated with reduced heat sterilization to make the taste much better, there is a risk of contamination of C. botulinum. It is difficult to prevent from preventing contamination of high heat tolerant score formers into these products during packaging. Thus, this study also aimed to assess risks in aseptic boiled rice with C.botulinum type A and B. Moreover, A rapid quantitative PCR assay (TaqMan assay) which quantifies C. botulinum type E by amplifying a 280-bp sequence from the botulinum neurotoxin type E (BONT/E)gene is developed. With this method, which uses the hydrolysis of an internal fluoregenic probe and monitors in real-time the increase in the intensity of fluorescence during PCR by using theABI PrismTM 7700 Sequence Detection System (SDS), it was possible to perform accurate and reproducible quantification of the C.botulinumtype E toxin gene.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] B.Kimura, S.Kawasaki, H.Nakano, T.Fujii: "Rapid quantitative PCR monitoring of growth of Clostridium botulunum type E in modified-atmosphere-packaged fish"Appl. Environ. Microbiol.. 67(1). 206-216 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 木村 凡: "包装食品の微生物制御(総説)"FFI JOUENAL. 188. 27-34 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 木村 凡: "包装食品における微生物学的品質確保(総説)"日本包装学会誌. 10. 111-118 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] B. Kimura. S. Kawasaki, H. Nakano and T. Fujii: "Rapid Quantitative PCR monitoring of growth of Clostridium botulinum type E in modified-atmosphere-packaged fish"Appl. Environ. Micro biol.. 67(1). 206-216 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] B. Kimura: "Microbial control of packaged foods"FFI Journal. 188. 27-34 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] B. Kimura: "Microbial quality of packaged foods"J. Pack. Sci.Technol. Jpn. 10. 111-118 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 木村 凡: "包装食品における微生物学的品質確保 (総説)"日本包装学会誌. 10. 111-118 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 木村 凡 (分担): "食品の衛生・安全包装 (分担:真空・ガス置換放送による品質保持)"幸書房. 229-249 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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