• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on superoxide dismutase and oxidative stress response in fishes

Research Project

Project/Area Number 12660186
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionNagasaki University

Principal Investigator

OSATOMI Kiyoshi  Faculty of Fisheries Associate Professor, 水産学部, 助教授 (40253702)

Co-Investigator(Kenkyū-buntansha) HARA Kenji  Faculty of Fisheries Professor, 水産学部, 教授 (10039737)
ISHIHARA Tadashi  Faculty of Fisheries Professor, 水産学部, 教授 (40039722)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
KeywordsSuperoxide dismutase / SOD / Fish / Purification / Cloning / Fish disease / Bacterial infection / Oxidative stress / スーパーオキシドジムターゼ
Research Abstract

Cu, Zn-superoxide dismutase (Cu, Zn-SOD) activities were compared in Japanese flounder Paralichthys olivaceus for four different kinds of bacterial infections. A significant increase in Cu, Zn-SOD levels was recorded in hepato-pancreas at the initial stages of Edwardsiella tarda (E. tarda) infection. It was suggested that the remarkable change of Cu, Zn-SOD activities was a characteristic phenomenon of E. tarda.
Cu, Zn-SOD and Mn-SOD were purified to homogeneity from Japanese flounder hepato-pancreas. On SDS-PAGE, the purified Cu, Zn-SOD and Mn-SOD gave single protein bands with molecular mass of 17.8 kDa and 26 kDa under reducing conditions, respectively. The N-terminal ammo acid sequences were determined for 25 amino acid residues (Cu, Zn-SOD) and 19 residues (Mn-SOD), the sequences were compared with other SODs. Reverse transcription-polymerase chain reaction of RNA from Japanese flounder hepato-pancreas was performed using primers designed according to the amino acid sequences determined, and we determined primary structure of Japanese flounder Cu, Zn-SOD and Mn-SOD. Furthermore, recombinant Cu, Zn-SOD (rCu, Zn-SOD) was expressed in Escherichia coli and was recovered as an active form.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] K.Osatomi: "Purification, N-terminal amino acid sequence, and some properties of Cu,Zn-super oxide dismutase from Japanese flounder (Paralichthys olivaceus) hepato-pancreas"Comparative Biochemistry and Physiology Part B. 128. 751-760 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 長富 潔: "細菌感染に伴うヒラメのCu,Zn-SOD活性の変化"日本水産学会誌. 68・2. 206-212 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Osatomi: "Purification and sequence of manganese superoxide dismutase from Japanese flounder (Paralichthys olivaceus) hepato-pancreas"Fisheries Science. 68. 1621-1622 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Osatomi, Kiyoshi: "Purification, N-terminal amino acid sequence, and some properties of Cu, Zn-superoxide dismutase from Japanese flounder (Paralichthys olivaceus) hepato-pancreas"Comparative Biochemistry and Physiology. Part B, 128. 751-760 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Osatomi, Kiyoshi: "Changes in Cu, Zn-SOD activity in Japanese flounder Paralichthys olivaceus with bacterial infection"Nippon Suisan Gakkaishi. 68(2). 207-213 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Osatomi: "Purification and sequence of manganese superoxide dismutase from Japanese flounder (Paralichthys olivaceus) hepato-pancreas"Fisheries Science. 68・2. 1621-1622 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Osatomi: "Purification, N-terminal amino acid sequence, and some properties of Cu, Zn-superoxide dismutase from Japanese flounder(Paralichthys olivaceus)hepato-pancreas"Comparative Biochemistry and Physiology Part B. 128. 751-760 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 長富 潔: "細菌感染に伴うヒラメのCu, Zn-SOD活性の変化"日本水産学会誌. 68・2. 206-212 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Osatomi,Y.Masuda,K.Hara,T.Ishihara: "Purification, N-terminal amino acid sequence, and some properties of Cu,Zn-superoxide dismutase from Japanese flounder (Paralichthys olivaceus) hepato-pancreas"Comparative Biochemistry and Physiology Part B. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi