| Project/Area Number |
12660254
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | KYOTO UNIVERSITY |
|---|
Principal Investigator |
NAGAO Yasumitsu Kyoto University, Graduate School of Agriculture, Assistant, 農学研究科, 助手 (80303874)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IMAI Hirosi Kyoto University, Graduate School of Agriculture, Professor, 農学研究科, 教授 (10303869)
徳永 智之 独立行政法人・農業生物資源研究所, 発生分化, 室長(研究職)
南 直次郎 (南 直治郎) 京都大学, 農学研究科, 助手 (30212236)
米川 博通 東京都臨床医学総合研究所, 副所長(研究職) (30142110)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
|---|
| Keywords | ES cell / mitochondrial DNA / sperm / germ-line chimera / totipotency / 精子受精能 / キメラ形成能 / 同胞異種 / 胚性幹細胞 |
|---|
| Research Abstract |
To examined the effect of mitochondrial DNA (mtDNA) on the germline-differentiating potency of ES cell line in the mouse, we investigated using the mice from standard and the mtDNA-congenic strain carrying different type of mtDNA under the same nuclear genetic background. We established the ES cell lines from standard strain C57BL/6J (B6) carring Mus musculus domesticus type mtDNA and from mtDNA-congenic strain C57BL/6Jmt^<spr> (B6 mt^<spr>) carring Mus spretus type mtDNA. When the ES cell lines derived from B6 and from B6 mt^<spr> were used to compare the germline-differentiating potency in the chimeric mice with ICR strain embryos, the germline-chimera mouse was obtained from only the B6 mt^<spr> ES cell line. We examined totipotency of the ES lines by tetraploid complemention. The tetraprloid-chimera mice were obtained from B6 ES cell line and from B6 mt^<spr> ES cell lines. From these results, we thought that the sperm derived from B6 mt^<spr> ES cell had high capacity of fertilization. When we compared B6 sperm with B6 mt^<spr> sperm by capacity of fertilization in vitro fertilization, B6 mt^<spr> sperm was higher than B6 sperm. The capacity of sperm from another mtDNA-congenic strain BALB/c mt^<mus> carring Mus musculus musculus type mtDNA was lower than the capacity of B6 sperm. The germ-line chimera mice were produced when B6 ES cells were injected into the blastosysts obtained from BALB/c mt^<mus>. It was suggested that mtDNA influenced the capacity of sperm from ES cell.
|
