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Identification of pathogenic genes in very virulent herpesviruses by analysis on single-nucleotide polymorphism.

Research Project

Project/Area Number 12660289
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionRakuno Gakuen University

Principal Investigator

ENDOH Daiji  Rakuno Gakuen University, School of Vet. Med., Ass. Pro., 獣医学部, 助教授 (40168828)

Co-Investigator(Kenkyū-buntansha) KON Yasuhiro  Hokkaido University, Grad. School of Vet. Med., Ass. Pro., 大学院・獣医学研究科, 助教授 (10178402)
KIRISAWA Rikio  Rakuno Gakuen University, School of Vet. Med., Ass. Pro., 獣医学部, 助教授 (70153252)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordssubtraction / herpesvirus / representational difference analysis / Marek's disease virus / preparation of genome / bovine herpesvirus 1 / porcine herpesvirus 1 / equine herpesvirus 1
Research Abstract

Although some vaccines are in use, new superior vaccines should be developed. On the development of vaccines, construction of non-virulent strain by destruction of virulent genes of virulent strains is basic strategy. But virulent genes have not been determined in many virulent viruses. Virulent genes are determined by referring interstrain polymorphism of genome between virulent and non-virulent strains.
In this study, we tried to determine virulent genes of very virulent herpesviruses using single-nucleotide polymorphisms (SNPs) as interstrain polymorphism markers, which is in the spotlight in human genomic research. On searching SNPs, preparation of viral fragments, which is suitable for detecting SNPs, is presumed to be most important step for detecting SNPs. Although many methods for detection of SNPs have been reported, method for rapid preparation of many viral DNA fragments have not been reported as a step for detection of SNPs. Thus, we focused on development of the method for preparation of many types of viral DNA fragments and also determination of appropriate size of the fragments to detect SNPs. Resultantly, we got the results below and achieved the first object of this research.
Firstly, we developed a method for preparation of vial DNA fragments from avian and mammalian herpesviruses independent on its genomic sequences. Secondly, we counted SNPs between virulent and non-virulent strains of herpesviruses and determined the sizes appropriate for determination of SNPs.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Endoh D: "Hypertonic treatment inhibits radiation-induced nuclear translocation of the Ku proteins G22p1 (Ku7O) and Xrcc5 (Ku8O) in rat fibroblasts"Radiat. Res. 155・2. 320-327 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kon Y: "Heat-shock resistance in experimental cryptorchid testis of mice"Mol. Reprod. Dev.. 58・2. 216-222 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Endoh D: "Application of representational difference analysis to genomic fragments of Marek's disease virus"J. Clin. Microbiol.. 38・12. 4310-4314 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Endoh D., Cho K. O., Tsukamoto K., Morimura T., Kon Y. and Hayashi M: "Application of representational difference analysis to genomic fragments of Marek's disease virus"J. Clin. Microbiol.. 38. 4310-4314 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Endoh D., Okui T., Kon Y. and Hayashi M: "Hypertonic treatment inhibits radiation-induced nuclear translocation of the Ku proteins G22p l (Ku70) and Xrcc5 (Ku80) in rat fibroblasts"Radiat. Res.. 155. 320-327 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kon Y. and Endoh D.: "Heat-shock resistance in experimental cryptorchid testis of mice"Mol. Reprod. Dev. 58. 216-222 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Endoh D: "Hypertonic treatment inhibits radiation-induced nuclear translocation of the Ku proteins G22p1 (Ku70) and Xrcc5 (Ku80) in rat fibroblasts"Radiat.Res. 155・2. 320-327 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kon Y: "Heat-shock resistance in experimental cryptorchid testis of mice"Mol.Reprod.Dev.. 58・2. 216-222 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Endoh D: "Application of representational difference analysis to genomic fragments of Marek's disease virus"J.Clin.Microbiol.. 38・12. 4310-4314 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Endoh,D.: "Application of representational difference analysis to genomic fragments of Marek's disease virus"J.Clin.Microbiol.. 38・12. 4310-4314 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kon,Y.: "Morphological study of metaphase-specific apoptosis in MRL mouse testis"Anat.Histol.Embryol.. 29・5. 313-319 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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