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REDUCTION OF IMMUNE MEMORY IN SPECIFIC IMMUNITY TO RHODOCOCCUS EQUI IN MICE MODEL

Research Project

Project/Area Number 12660290
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionKitasato University

Principal Investigator

TSUBAKI Shiro  KITASATO UNIVERSITY, SCHOOL OF VETERINARY MEDICINE AND ANIMAL SCINECES, PROFESSOR, 獣医畜産学部, 教授 (70050507)

Co-Investigator(Kenkyū-buntansha) KAKUDA Tsutomu  KITASATO UNIVERSITY, SCHOOL OF VETERINARY MEDICINE AND ANIMAL SCINECES, ASSISTANT PROFESSOR, 獣医畜産学部, 助手 (80317057)
TAKAI Shinji  KITASATO UNIVERSITY, SCHOOL OF VETERINARY MEDICINE AND ANIMAL SCINECES, ASSOCIATE PROFESSOR, 獣医畜産学部, 助教授 (80137900)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsRHODOCCUS EOUT / PROTECTIVE IMMUNITY / MEMORY CELLS / MICE / CYTOKINES / IL-10 / 特異免疫応答
Research Abstract

Immunization with live virulent R. equi conferred protection against virulent organisms in mice, whereas heat-killed virulent or live avirulent bacteria offered no protection. The protective immunity was observed 5 days after the sublethal inoculation with live ATCC 33701, and the most effective clearance of challenged bacteria was found in the mice primed at 9 days before the challenge. Immune memory by the live virulent R. equi reduced for a few weeks. The purpose of this study is to elucidate the mechanism of the reduction of R. equi-specific immune memory in mice model. To measure the production of IFN-γ and TNF-α from immune spleen cells, mice were immunized with live virulent R. equi for 7, 17, 21, and 28 days. The highest production of IFN-γ and TNF- a from immune spleen cells after stimulation with R. equi antigens was observed in mice immunized for 7 days, and the production level of the spleen cell from mice immunized for 17 days became the same as that from normal mice. Sple … More en cells from mice immunized for 7 or 14 days were transfer to normal mice and the mice were challenged with virulent R. equi. The transfer immunity was effective in mice received spleen cell primed for 7 days, but not for 14 days. Immunized mice for 7 or 21 days with virulent R. equi were inoculated with anti-IL10 monoclonal antibody, and then challenged with virulent R. equi. There was no effect of anti-IL-10 antibody on the clearance of R. equi in the organs as compared with those of none-treated immune mice. On the other hand, in vitro experiment with anti-IL-10 monoclonal antibody showed effective function of IL-10 on the production of IFN-γ and TNF-α from immune spleen cells. Expression of CD44, CD62L, and CD45RB antigens on the spleen cells were analyzed by flow cytometory analysis using anti-CD44, CD62L, and CD45RB antibodies. However, there was no change between spleen T cells from immunized mice for 7 and 21 days. In conclusion, IL-10 might play a key role in the establishment and reduction of immune memory in R. equi infection in mice, however, the mechanism of inactivation or tolerance of T cells by R. equi infection remain unclear. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Morton, AC et al.: "Epidemiology Of Rhodococcus equt Strains On Thoroughbred Horse Farms"Appl Environ Microbiol. 67. 2167-2175 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takai, S., et al.: "Prevalence of virulent Rhodococcus equi in soil from 5 R.equi-endemic horse-breeding farms andrestriction fragment length polymorphisms of virulence plasmids in isolates from soil and infected foals in Texas"J.Vet.Diag.Invest.. 13. 489-494 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takai, S., et al.: "Rhodococcus equi pneumonia in a crossbred foal in Kumamoto : clinical isolates contained new virulence plasmids, 90-kb type III and type IV"Vet.Microbiol.. 82. 373-381 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Horowitz, ML et al.: "Application of Sartwell's Model (Logarithmic-normal Distribution of Incubation period) to age at onset andage at death of foals with Rhodococcus equi pneumonia as evidence of perinatal infection"J.Vet.Intern.Med.. 15. 171-175 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Flynn, O. et al.: "Virulence-associated protein characterisation of Rhodococcus equi isolated from bovine lymph nodes"Vet.Microbiol.. 78. 221-228 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takai, S. et al.: "Prevalence of virulent Rhodococcus equi in isolates from soil collected from 2 horse farms in South Africa and restriction fragment length polymorphisms of virulence plasmids in the isolates from infected foals, dog and monkey"Onderstepoort J.Vet.Res.. 68. 105-110 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Byrne, B. A., J. F. Prescott, G. H. Palmer, S. Takai, V. M. Nicholson, D. C. Alperin, S. A. Hines: "The virulence plasmid of Rhodococcus equi contains an inducible gene family encoding secreted protein"Infect. Immun.. 69. 650-656 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Flynn, O., F. Quigley, E. Costello, D, O'Grady, A. Gogarty, J. McGuirk, and S. Takai: "Virulence-associated protein characterisation of Rhodococcus equi isolated from bovine lymph nodes"Vet. Microbiol.. 78. 221-228 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takai, S., M. M. Henton, J. A. Picard, A. J. Guthrie, H. Fukushi, and C. Sugimoto: "Prevalence of virulent Rhodococcus equi in isolates from soil collected from 2 horse farms in South Africa and restriction fragment length polymorphisms of virulence plasmids in the isolates from infected foals, dog and monkey"Onderstepoort J. Vet. Res.. 68. 105-110 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takai, S., M. K. Chaffin, N. D. Cohen, M. Hara, M. Nakamura, T. Kakuda, Y. Sasaki, S. Tsubaki, and R. J. Martens: "Prevalence of virulent Rhodococcus equi in soil from 5 R.eqtii-endemic horse-breeding farms and restriction fragment length polymorphisms of virulence plasmids in isolates from soil and infected foals in Texas"J. Vet. Diag. Invest.. 13. 489-494 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takai, S., N. Murata, T. Kakuda, Y. Sasaki, S. Tsubaki, R. Kudo, and N. Narematsu: "Rhodococcus equi pneumonia in a crossbred foal in Kumamoto: clinical isolates contained new virulence plasmids, 90-kb type III and type IV"Vet. Microbiol.. 82. 373-381 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Byrne, B.A.et al.: "The virulence plasmid of Rhodococcus equi contains an inducible gene family encoding secreted protein"Infect. Immun. 69. 650-656 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Flynn, O., et al.: "Virulence-associated protein characterisation of Rhodococcus equi isolated from bovine lymph nodes"Vet. Microbiol. 78. 221-228 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takai, S., et al.: "Prevalence of virulent Rhodococcus equi in isolates from soil collected from 2 horse farms in South Africa and restriction fragment length polymorphisms of virulence plasmids in the isolates from infected foals, dog and monkey"Onderstepoort J.Vet. Res.. 68. 105-110 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takai, S., et al.: "Prevalence of virulent Rhodococcus equi in soil from 5 R.equi-endemic horse-breeding farms and restriction fragment length polymorphisms of virulence plasmids in isolates from soil and infected foals in Texas"J. Vet. Diag. Invest. 13. 489-494 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takai, S., et al.: "Rhodococcus equi pneumonia in a crossbred foal in Kumamoto : clinical isolates contained new virulence plasmids, 90-kb type III and type IV"Vet. Microbiol. 82. 373-381 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takai,S.: "Pathogenicity of Rhodococcus equi expressing a virulence-associated 20-kDa antigen in foals."Vet.Microbiol.. 76. 71-80 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takai,S.: "Some epidemiological aspects of Rhodococcus equi infection in foals in Japan : A review of 108 cases in 1992-1998."J.Equine Sci.. 11. 7-14 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takai,S: "Isolation of virulent Rhodococcus equi from native Japanese horses."Comp.Immun.Microbiol.Infect.Dis.. 24. 123-133 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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