| Project/Area Number |
12660295
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物資源科学
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
SUGIMOTO Manabu OKAYAMA UNIVERSITY, Research Institute for Bioresources, Assistant Professor, 資源生物科学研究所, 助手 (20216336)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | phospholipid hydroperoxide glutathione peroxidase / oxidative stress / salt stress / プロモーター |
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| Research Abstract |
The gene that includes the promoter region of the phospholipid hydroperoxide glutathione peroxidase (PHGPX)-like protein was cloned from Arabidopsis thaliana. The gene was subcloned into a plasmid, pBI101, resulting the promoter gene was fused with the β-glucuronidase (GUS) gene. The fused gene was transferred to the root of the pea by the particle bombardment system. Transient expression of the GUS was shown in the roots cultured by the MS medium containing 0, 50, and 100mM NaCl. This result suggests that the PHGPX-like gene is not induced by oxidative/salt stress.
A cDNA encoding barley PHGPX-like protein was expressed in Escherichia coli cells to produce an extra protein. The PHGPX was not detected in the cell-free extract prepared from the clone cells when tert-butyl hydroperoxide, cumene hydroperoxide, and hydrogen peroxide were used as a substrate. The clone cells were assayed for their ability to grow in the medium containing NaCl. The clone cells were much more tolerant to NaCl stress than the host cells, suggesting that the PHGPX-like protein would be a good candidate component of anti-salt/oxidative enzyme in plants.
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