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Nectin : An Adhesion Molecule Involved in Formation-of Synaps

Research Project

Project/Area Number 12670014
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionMie University (2001)
Kyoto University (2000)

Principal Investigator

MIZOGUCHI Akira  Mie University, Faculty of Medicine, Professor, 医学部, 教授 (90181916)

Co-Investigator(Kenkyū-buntansha) KIMURA Kazushi  Kyoto University, Graduate School of Medicine, Assistant, 医学研究科, 助手 (20314180)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsNectin / afadin / cadherin / puncta adherentia junctions / synaptic junctions / シナプス / 可塑性 / Puncta Adherentia Junction / アドヘレンスジャンクション / タイトジャンクション
Research Abstract

The nectin-afadin system is a novel cell-cell adhesion system that organizes adherens junctions cooperatively with the cadherin-catenin system in epithelial cells. Nectin is an immunoglobulin-like adhesion molecule, and afadin is an actin filament-binding protein that connects nectin to the actin cytoskeleton. Nectin has four isoforms (-1, -2, -3, and -4). Each nectin forms a homo-cis-dimer followed by formation of a homo-trans-dimer, but nectin-3 furthermore forms a hetero-trans-dimer with nectin-1 or -2, and the formation of each hetero-trans-dimer is stronger than that of each homo-trans-dimer. We show here that at the synapses between the mossy fiber terminals and the dendrites of pyramidal cells in the CA3 area of adult mouse hippocampus, the nectin-afadin system colocalizes with the cadherin-catenin system and that nectin-1 and -3 asymmetrically localize at the presynaptic and postsynaptic sides of puncta adherentia junctions, respectively. During development, nectin-1 and -3 asymmetrically localize not only at puncta adherentia junctions but also at synaptic junctions. Inhibition of the nectin-based adhesion by an inhibitor of nectin-1 in cultured rat hippocampal neurons results in decrease in size and concomitant increase in number of synapses. These results indicate a key role of the nectin-afadin system in formation of synapses.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Mizoguchl A, Nakanishl H, Kimura K, Matsubara K, el.: "Nectin : an adhesion molecule involved in formation of synapses"J Cell Biol. 156(3). 555-565 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mizoguchi A, Eguchi N, Kimura K, Kiyohara Y, el.: "Dominant localization of prostaglandin D receptors on arachnoid trabecular cells in mouse basal forebrain and their invement in 〜"Proc Natl Acad Sci USA. 25;98(20). 11674-11679 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oh J, Takahashi R, Kondo S, Mizoguchi A, Adachi E, el.: "The membrane-anchored MMP inhibitor RECK is a key regulator of extracellular matrix integrity and angiogenesis"Cell. 107(6). 789-800 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka M, Miyoshi J, Ishizaki H, Mizoguchi A, el.: "Role of rab3 gdp/gtp exchange protein in synaptic vesicle trafficking at the mouse neuromuscular junction"Mol Biol Cell. 12(5). 1421-1430 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimura H, Okazaki T, Tanaka Y, Mizoguchi A, el.: "Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice"Science. 291(5502). 319-322 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsuoka T, Mizoguchi A, Tanaka H, Takahashi Y, el.: "Prostaglandin D2 as a mediator of allergic asthma"Science. 287. 2013-2017 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mizoguchi A, Nakanishi H, Kimura K., Matsubara K, Ozaki-Kuroda K, Katata T, Honda T, Kiyohara Y, Heo K, Higashi M, Tsutsumi T, Sonoda S, Ide C, Takai Y.: "Nectin : an adhesion molecule involved in formation of synapses"J Cell Biol. 156(3). 555-565 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oh J, Takahashi R, Kondo S, Mizoguchi A, Adachi E, Sasahara RM, Nishimura S, Imamura Y, Kitayama H, Alexander DB, Ide C, Horan TP, Arakawa T., Yoshida H, Nishikawa S, Itoh Y, Seiki M, Itohara S, Takahashi C, Noda M.: "The membrane-anchored MMP inhibitor RECK is a key regulator of extracellular matrix integrity and angiogenesis"Cell. 107(6). 789-800 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishiki T, Nihonmatsu I, Tsuhara Y, Kawasaki M, Sekiguchi M, Sato K, Mizoguchi A, Takahashi M.: "Distribution of soluble N-ethylmaleimide fusion protein attachment proteins (SNAPs) in the rat nervous system"Neuroscience. 107(3). 363-371 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mizoguchi A, Eguchi N, Kimura K, Kiyohara Y, Qu WM, Huang ZL, Mochizuki T, Lazarus M, Kobayashi T, Kaneko T, Narumiya S, Urade Y, Hayaishi O.: "Dominant localization of prostaglandin D receptor's on arachnoid trabecular cells in mouse basal forebrain and their invement in the regulation of non-rapid eye movement sleep"Proc Natl Acad Sci USA. 25 ; 98(20). 11674-11679 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshida Y, Morita K, Mizoguchi A, Ide C, Miyachi Y.: "Altered expression of occludin and tight junction formation in psoriasis"Arch Dermatol Res.. 293(5). 239-244 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka M, Miyoshi J, Ishizaki H, Togawa A, Ohnishi K, Endo K, Matsubara K, Mizoguchi A, Nagano T, Sato M, Sasaki T, Takai Y.: "Role of rab3 gdp/gtp exchange protein in synaptic vesicle trafficking at the mouse neuromuscular junction"Mol Biol Cell. 12(5). 1421-1430 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mazaki Y, Hashimoto S, Okawa K, Tsubouchi A, Nakamura K, Yagi R, Yano H, Kondo A, Iwaniatsu A, Mizoguchi A, Sabe H.: "An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is inved in subcellular localization of paxillin and actin cytoskeletal organization"Mol Biol Cell. 12(3). 645-662 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ide C, Kitada M, Chakrabortty S, Taketomi M, Matsumoto N, Kikukawa S, Mizoguchi A, Kawaguchi S, Endoh K, Suzuki Y.: "Grafting of choroid plexus ependymal cells promotes the growth of regenerating axons in the dorsal funiculus of rat spinal cord : a preliminary report"Exp Neurol. 167(2). 242-251 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimura H, Okazaki T, Tanaka Y, Nakatani K, Hara M, Matsumori A, Sasayama S, Mizoguchi A, Hiai H, Minato N, Honjo T.: "Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice"Science. 12 ; 291(5502). 319-322 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Peng YF, Mandai K, Sakisaka T, Okabe N, Yamamoto Y, Yokoyama S, Mizoguchi A, Shiozaki H, Monden M, Takai Y.: "Ankycorbin : a novel actin cytoskeleton-associated protein"Genes Cells. 5(12). 1001-1008 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishida A, Takahashi M, Tanihara H, Nakano I, Takahashi J, Mizoguchi A, Ide C, Honda Y.: "Incorporation and differentiation of hippocampus-derived neuronal stem cells transplanted in injured adult rat retina"Invest Ophthalmol Vis Sci. 41. 4268-4274 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Beuckmann CT, Lazarus M, Gerashchenko D. Mizoguchi A, Nomura S, Mohri I, Uesugi A, Kaneko T, Mizuno N, Hayaishi O, Urade Y.: "Cellular localization of lipocalin-type prostaglandin D synthase (beta-trace) in the central nervous system of the adult rat"J Comp Neurol. 428(1). 62-78 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ishizaki H, Miyosi J, Kamiya H, Togawa A, Tanaka M, Sasaki T, Endo K, Mizoguchi A, Ozawa S, Takai Y.: "Role of Rab GDP dissociation inhibitor a in regulating plasticity of hippocampal neurotransmission"Proc Natl Acad Sci USA. 97. 11587-11592 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Toda H, Takahashi J, Mizoguchi A, Koyano K, Hashimoto N.: "Neurons Generated from Adult Rat Hippocampal Stem Cells Form Functional Glutamatergic and GABAergic Synapses in Vitro"Exp Neurol. 165. 66-76 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shirasu M, Kimura K, Kataoka M, Takahashi M, Okajima S, Kawaguchi S, Hirasawa Y, Ide C, Mizoguchi A.: "VAMP-2 promotes nuerite elongation and SNAP-25A increases neurite sprouting in PC12 cells"Neurosci Res. 37. 265-275 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirao K, Hata Y, Deguchi M, Yao I, Ogura M, Rokukawa C, Kawabe H, Mizoguchi A, Takai Y.: "Association of synapse-associated protein 90/ postsynaptic density-95-associated protein (SAPAP) with neurofilaments"Genes Cells. 5. 203-210 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Satoh-Horikawa K, Nakanishi H, Takahashi K, Miyahara M, Nishimura M, Tachibana K, Mizdguchi A, Takai Y.: "Nectin-3, a new member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities"J Biol Chem. 275. 10291-10299 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishioka H, Mizoguchi A, Nakanishi H, Mandai K, Takahashi K, Kimura K, Satoh-Moriya A, Takai Y.: "Localization of 1-afadin at puncta adhaerentia-like junctions between the mossy fiber terminals and the dendritic trunks of pyramidal cells in the adult mouse hippocampus"J Comp Neurol. 424. 297-306 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsuoka T, Hirata M, Tanaka H, Takahashi Y, Murata T, Kabashima K, Sugimoto Y, Kobayashi T, Ushikubi F, Aze Y, Eguchi N, Urade Y, Yoshida N, Kimura K, Mizoguchi A, Honda Y, Nagai H, Narumiya S.: "Prostaglandin D2 as a mediator of allergic asthma"Science. 287. 2013-2017 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirao K, Hata Y, Yao I, Deguchi M, Kawabe H, Mizoguchi A, Takai Y.: "Three isoforms of synaptic scaffolding molecule and their characterization. Multimerization between the isoforms and their interaction with N-methyl-D-aspartate receptors and SAP90/PSD-95-associated protein"J Biol Chem. 275. 2966-2972 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujita A, Nakamura K, Kato T, Watanabe N, Ishizaki T, Kimura K, Mizoguchi A, Narumiya S.: "Ropporin, a sperm-specific binding protein of rhophillin, that is localized in the fibrous sheath of sperm flagella"J Cell Sci. 113. 103-112 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mizoguchi A, Nakanishi H, Kimura K, Matsubara K, el.: "Nectin:an adhesion molecule involved in formation of synapses"J Cell Biol. 156(3). 555-565 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mizoguchi A, Eguchi N, Kimura K, Kiyohara Y, el.: "Dominant localization of prostaglandin D receptors on arachnoid trabecular cells in mouse basal forebrain and their invement in〜"Proc Natl Acad Sci USA. 25;98(20). 11674-11679 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Oh J, Takahashi R, Kondo S, Mizoguchi A, Adachi E, el.: "The membrane-anchored MMP inhibitor RECK is a key regulator of extracellular matrix integrity and angiogenesis"Cell. 107(6). 789-800 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tanaka M, Miyoshi J, Ishizaki H, Mizoguchi A, el.: "Role of rab3 gdp/gtp exchange protein in synaptic vesicle trafficking at the mouse neuromuscular junction"Mol Biol Cell. 12(5). 1421-1430 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishimura H, Okazaki T, Tanaka Y, Mizoguchi A, el.: "Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice"Science. 291(5502). 319-322 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsuoka T, Mizoguchi A, Tanaka H, Takahashi Y, el.: "Prostaglandin D2 as a mediator of allergic asthma"Science. 287. 2013-2017 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Satoh-Horikawa,K.,Nakanishi,H,Mizogachi,A., et al.: "Nectin-3. a new member of immunoglobalin fibre cell adhesion molecules that shows homophilic and heterophilie cell-cell adhesion activities."Journal of Biological Chemistry. 275. 10291-10299 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirao,K.,Hata,Y.,Mizoguchi,A., et al: "Three soforms of synaptic scaffolding molecule and their characterization Microthimenzation hetween the isoforms and their infiction with N-methyl-O-aspartale receptor and cacer protein"Journal of Biological Chemistry. 275. 2966-2972 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirao,K.,Hata.Y.,Mizagachi,A., et al: "Association of synapse-associated protein 90/postsynaptic chemity-95-associated protein(SAPAP) wigh rearofilaments"Genes Cells. 5. 203-210 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsuoka,T.,Hirata,M.,Migigachi,A., et al: "Prostaglandin D2 as a mediator of allergic asthma."Science. 287. 2013-2017 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shirusu,H,Kimara,K.,Mizoguchi,A., et al: "VAMP-Z promotes neurite eleongetion and SNAP-25A increases nearite spreating in PCIZ cells"Nearoscience Research. 37. 265-275 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nishioko,H,Mizogachi,A, et al: "Localization of 1-atictin at punchi adhaerentic the , untions the messy their cerminals and the dentric of pyrimilat cells in the adult mouse hyppocampus"Journal of Companatice Neunology. 424. 297-306 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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