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The proliferation and the differentiation of the fundic gland constituent cells by means of immunohistochemical and molecular biological methods

Research Project

Project/Area Number 12670023
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKagoshima University

Principal Investigator

MURATA Fusayoshi  Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (60020765)

Co-Investigator(Kenkyū-buntansha) MATSUSHITA Sachie  Kagoshima University, Faculty of Medicine, Research Associate, 医学部, 助手 (80325787)
HASUI Kazuhisa  Kagoshima University, Faculty of Medicine, assistant professor, 医学部, 講師 (70198703)
TSUYAMA Shinichiro  Kagoshima University, Faculty of Medicine, associate professor, 医学部, 助教授 (30041346)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsMucous neck cell / Intermediate cell / Chief cell / glycoconjugate / lectin / immunohistochemistry / Pepsinogen C / in situ hybridization / 高圧凍結 / 凍結置換 / H-K-ATPase / 壁細胞 / in situ hybridization
Research Abstract

Mucous neck cell is a seromucous cell and chief cell is a serous cell. Intermediate cell between mucous neck cell and chief cell is also discemible in the fundic gland. The granule of intermediate cell has a biphasic structure consist of serous and mucous portions. Using electron microscopical, glycoconjugate histochemical, immunohistochemical and in situ hybridization techniques, we have been studying the differentiation of various cell types in the fundic gland. PA-TCH-SP staining, lectin colloidal gold staining and HIK 1083 staining labeled mucous neck cell, intermediate cell in relation to its glycoconjugate concentration in the granule. In the intermediate cell, only mucous portion of this granule was labeled by these staining but chief cell granule was not labeled by these staining. Immunohistochemistry of pepsinogen C stained these three types of cells in crescent manner of labeling density. In protein level, it has been verified that the mucous neck is the precursor of the chief cell. Messenger RNA of pepsinogen C was also ascertained in mucous neck cell and chief cell. This means that our proposal has also been ascertained in the gene level

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Hidaka E. et al.: "Coexistence of gland mucous cell-type mucin and lysozyme in gastric gland mucous cells"Histochem, Cell Biol.. 113. 91-98 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yang D-H. et al.: "Expression on N-acetylglucosamine residues in developing rat fundic gland cells"Histochemical J.. 32. 187-193 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 村田長芳 他: "高圧凍結"電子顕微鏡. 35. 109-110 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yang D-H. et al.: "The expression of gastric H^+-K^+-ATPase mRNA and protein in developing rat fundic gland"Histochemical J.. 33. 159-166 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 村田長芳: "高圧凍結:過去、現在、未来"電子顕微鏡. 36. 95-98 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 菅沼龍夫 他: "高圧凍結・凍結置換法による形態と組織化学"電子顕微鏡. 36. 99-101 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tsuyama S. et al.: "Cytochemical investigation of gastric gland component cells with high-pressure freezing followed by freeze-substitution and hydrophilic resin embedding"Anat, Sci, Int.. 77. 74-83 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasui K. et al.: "Immunohistochemical analysis of the pathogenicity of helicobacter pylori infection : excess nitric oxide induced indirectly by Lewis X and Y is the cause of the pathogenicity of helicobacter pylori infestation in the stomach"Acta Histochem, Cytochem.. 35. 93-100 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 蓮井和久 他: "小胞体"著書.学際企画. 39-41 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 村田長芳 他: "ゴルジ装置"著書.学際企画. 46-47 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 村田長芳 他: "複合糖質の局在と機能"細胞. 34. 596-599 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tsuyama S. et al.: "Cytochemistry of gastric parietal cells with high-pressure freezing followed by freeze-substitution"J Electron Microsc.. 52(In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hidaka E. et al.: "Coexistence of gland mucous cell-type mucin and lysozyme in gastric gland mucous cells"Histochem Cell Biol. 113(2). 91-98 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yang D-H. et al.: "Expression on N-acetylglucosamine residues in developing rat fundic gland cells"Histochemical J. 32(3). 187-193 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Murata F. et al.: "High pressure freezing"Electron Microsc. 35(2). 109-110 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yang D-H. et al.: "The expression of gastric H^+-K^+-ATPase mRNA and protein in developing rat fundic gland"Histochemical J. 33(3). 159-166 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Murata F: "High pressure freezing: past, present, future"Electron Microsc. 36(2). 95-98 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suganuma T. et al.: "High pressure freezing/freeze substitution and its application onto histochemistry"Electron Microsc. 36(2). 99-101 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tsuyama S. et al.: "Cytochemical investigation of gastric gland component cells with high-pressure freezing followed by freeze-substitution and hydrophilic resin embedding"Anat. Sci. Int.. 77. 74-83 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasui K. et al.: "Immunohistochemical analysis of the pathogenicity of helicobacter pylori infection: excess nitric oxice induced indirectly by Lewis X and Y is the cause of the pathogenicity of helicobacter pylori infestation in the stomach"Acta Histochem. Cytochem.. 35. 93-100 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Murata F. et al.: "The localization of glycoconjugates and its function"Saibou. 34(14). 30(596)-33(599) (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tsuyama S. et al.: "Cytochemistry of gastric parietal cells with high-pressure freezing followed by freeze-substitution"J. Electron Microsc.. 52(2) (In press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasui K. et al.: "Endoplasmic reticulum, in Monograph"Gakusaikikaku. 39-41 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Murata F. et al.: "The Golgi apparatus, in Monograph"Gakusaikikaku. 46-47 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tsuyama S: "Cytochemical investigation of gastric gland component cells with high-pressure freezing followed by freeze-substitution and hydrophilic resin embedding"Anatomical Science International. 77. 74-83 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hasui K: "Immunohistochemical analysis of the pathogenicity of helicobacter pylori infection : excess nitric oxice induced indirectly by lewis X and Y is the cause of the pathogenicity of helicobacter pylori infestation in the stomach"Acta Histochem Cytochem. 35. 93-100 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] 蓮井和久: "小胞体"日本臨床電子顕微鏡学会編. 39-41 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 村田長芳: "ゴルジ装置"日本臨床電子顕微鏡学会編. 46-47 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 村田長芳: "複合糖質の局在と機能"細胞. 34. 596-599 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tsuyama S: "Cytochemistry of gastric parieta cells with high-pressure freezing followed by freeze substitution"Journal of Electron Microscopy. (In press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Yang D-H: "The expression of gastric H^+-K^+-ATPase mRNA and protein inDeveloping rat fundic gland"Hiatochemical J. 33・3. 159-166 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 菅沼龍夫: "高圧凍結・凍結置換法による形態と組織化学"電子顕微鏡. 36・2. 99-101 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 村田長芳: "高圧凍結:過去、現在、未来"電子顕微鏡. 36・2. 95-98 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hidaka E: "Coexistence of gland mucous cell-type mucin and lysozyme in gastric gland mucous cells."Histochem Cell Biol. 113・2. 91-98 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yang D-H: "Expression of N-acetylglucosamine residues in developing rat fundic gland cells."Histochemical J. 32・3. 187-193 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 村田長芳: "高圧凍結"電子顕微鏡. 35・2. 109-110 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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