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MOLECULAR AND CELL BIOLOGICAL STUDY ON CELLULAR RESPONSE TO MECHANICAL STRESS

Research Project

Project/Area Number 12670032
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionUNIVERSITY OF TSUKUBA

Principal Investigator

TAKEMASA Tohru  UNIVERSITY OF TSUKUBA, INSTITUTE OF HEALTH & SPORT SCIENCES, ASSISTANT PROFESSOR, 体育科学系, 講師 (50236501)

Co-Investigator(Kenkyū-buntansha) HAGA Shukoh  UNIVERSITY OF TSUKUBA, INSTITUTE OF HEALTH & SPORT SCEINCES, PROFESSOR, 体育科学系, 教授 (80093102)
YAMASHITA Kazuo  NIPPON MEDICAL SCHOOL, DEPARTMENT OF ANATOMY I, PROFESSOR, 解剖学第一講座, 教授 (70022796)
SUGIMOTO Keiji  NIPPON MEDICAL SCHOOL, DEPARTMENT OF ANATOMY I, ASSOCIATE PROFESSOR, 解剖学第一講座, 助教授 (20104002)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsMECHANICAL STRESS / STRESS FIBER / ACTIN / BIOMECHANICS / MOLECULAR AND CELLULAR BIOLOGY / PATENT / CYCLIC STRAIN / EXERCISE / 転写
Research Abstract

The stress fiber is one of the most prominent cytoskeletal components that contribute to the maintenance of cell architecture. It has generally been believed that upon cyclic stretching, both cells and their stress fibers become perpendicularly aligned to the direction of stretching. However, using our newly developed stretching device, we have recently found the contrary evidence that stress fibers in endothelial cells rapidly become rearranged at a specific oblique angle relative to the direction of stretching. In light of this finding, we attempted to establish the explanation for such a phenomenon either experimentally or theoretically. It was thus revealed that stress fibers align at a particular angle so as to minimize their length alterations in cyclic deforming fields. Rearrangement of the stress fibers at this angle probably occurs as a result of avoiding compressive stress and may be interpreted as a way of reducing the mechanical stress to which. they are subjected during the deformation. This hypothesis explains well the reason not only for the survival of the stress fibers at a particular oblique angle, but also for the reduced numbers of stress fibers found, at the other angles on cyclic deforming fields. Moreover, theoretical study supports this hypothesis of intracellular stress fiber orientation under cyclic deformation.
We also expand our study from microscopic level to macroscopic level, that is, individual human being. Exercise with skeletal muscle contraction can be considered to mechanical stress from the standpoint of cells in body. We studied cellular response to both endurance and resistance exercise.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Keiji Sugimoto, Sachiko Fujii, Tohru Takemasa, Kazuo Yamashita: "Detection of intracellular nitric oxide using a combination of aldehyde fixatives with 4,5-diaminofluorescein diacetate"Histochemistry and Cell. 113. 341-347 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroshi Yamada, Tohru Takemasa, Takami Yamaguchi: "Theoretical study of intracellular stress fiber orientation under cyclic deformation"Journal of Biomechanics. 33・11. 1501-1505 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koji Toshinai, Shukoh Haga, Hiromi Miyazaki, Tohru Takemasa, Takeo Nomura, Takako Kizaki, Hideki Ohno: "Effects of different intensity and duration of exercise with the same oxygen consumption on the ability of neutrophils to generate superoxide anion radicals in humans."Adv.Exerc.Sports Physiol.. 6・3. 91-125 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroshi YAMADA, Daisaku MORITA, Jin MATSUMURA, Tohru TAKEMASA, Takami YAMAGUCHI: "Numerical Simulation of Stress Fiber Orientation in Cultured Endothelial Cells under Biaxial Cyclic Deformation using the Strain Limit Hypothesis"JSME International Journal, Series C. 45・4. 880-888 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 武政 徹(編著者の一人): "SeriesニューバイオフィジックスII第5巻 細胞の形と運動(担当編集委員:宝谷紘一, 神谷 律)(日本生物物理学会編)"共立出版株式会社. 180 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroshi Yamada, Tohru Takemasa, Takami Yamaguchi: "Clinical Application of Computational Mechanics for the Cardiovascular System"Springer-Verlag Tokyo. 382 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Keiji Sugimoto, Sachiko Fujii, Tohru Takemasa, Kazuo Yamashita: "Detection of intracellular nitric oxide using a combination of aldehyde fixatives with 4,5-diaminofluorescein diacetate"Histochemistry and Cell Biology. vol.113. 341-347 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroshi Yamada, Tohru Takemasa, Takami Yamaguchi: "Theoretical study of intracellular stress fiber orientation under cyclic deformation"Journal of Biomechanics. vol.33(11). 1501-1505 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koji Toshinai, Shukoh Haga, Hiromi Miyazaki, Tohru Takemasa, Takeo Nomura, Takako Kizaki, Hideki Ohno: "Effects of different intensity and duration of exercise with the same oxygen consumption on the ability of neutrophils to generate superoxide anion radicals in humans"Adv.Exerc.Sports Physiol.. vol.6(3). 91-95 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroshi YAMADA, Daisaku MORITA, Jin MATSUMURAI Tohru TAKEMASA, Takami YAMAGUCHI: "Numerical Simulation of Stress Fiber Orientation in Cultured Endothelial Cells under Biaxial Cyclic Deformation using the Strain Limit Hypothesis"JSME International Journal, Series C. vol.45(4). 880-888 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shukoh HAGA, Kazuki ESAKI, Kouji TOSHINAI, Takashi KINUGASA, Tohru TAKEMASA, Etsuo UEYA, Takafumi HAMAOKA, Toshihito KATSUMURA, Takako KIZAKI, Hideki OHNO: "Cardiac output and stroke volume at anaerobic threshold during walking exercise in healthy aged subjects"Adv.Exerc.Sports Physiol.. vol.9(1). 37-43 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroshi Yamada, Tohm Takemasa, Takami Yamaguchi: "Orientation Response of Stress Fibers in Cultured Cells under Biaxial Cyclic Stretch: Hypothesis and Theoretical Prediction.Clinical Application of Computational Mechanics for the Cardiovascular System (T Yamaguchi ed.,)"Springer-Verlag Tokyo.. 273-282 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Keiji Sugimoto, Sachiko Fujii, Tohru Takemasa, Kazuo Yamashita: "Detection of intracellular nitric oxide using a combination of aldehyde fixatives with 4,5-diaminofluorescein diacetate"Histochemistry and Cell. 113. 341-347 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiroshi Yamada, Tohru Takemasa, Takami Yamaguchi: "Theoretical study of intracellular stress fiber orientation under cyclic deformation""Journal of Biomechanics. 33・11. 1501-1505 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 武政 徹(編著者の一人): "Series ニューバイオフィジックス ‖ 第5巻 細胞の形と運動(担当編集委員 : 宝谷紘一、神谷 律)"共立出版株式会社(日本生物物理学会編). 180 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiroshi Yamada, Tohru Takemasa, Takami Yamaguchi(編著者の一人): "Clinical Application of Computational Mechanics for the Cardiovascular System"Springer-Verlag Tokyo. 382 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Keiji Sugimato et al.: "Detection of intracellular mtricoxide using combination of aldehyde fixafives with 4-5-damino fluorescein diacetate"Histochemistry and cell Biology. 113. 341-347 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hioshi Yamada et al.: "Theoretical study of inhacethar stress fiber onentation under cyclic deformation"Jouranl of Biomechanics. 33(11). 1501-1505 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 武政徹: "series ニューベイオフィジックスII第5巻細胞の形と運動"共立出版株式会社. 19 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirosh Yamada, Tohir Takemasa…: "Clinical Applecation of Cempntational Mechanics for the Cardiorenal system"Springer Verlag Tokyo. 10 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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