MOLECULAR AND CELL BIOLOGICAL STUDY ON CELLULAR RESPONSE TO MECHANICAL STRESS
| Project/Area Number |
12670032
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | UNIVERSITY OF TSUKUBA |
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Principal Investigator |
TAKEMASA Tohru UNIVERSITY OF TSUKUBA, INSTITUTE OF HEALTH & SPORT SCIENCES, ASSISTANT PROFESSOR, 体育科学系, 講師 (50236501)
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| Co-Investigator(Kenkyū-buntansha) |
HAGA Shukoh UNIVERSITY OF TSUKUBA, INSTITUTE OF HEALTH & SPORT SCEINCES, PROFESSOR, 体育科学系, 教授 (80093102)
YAMASHITA Kazuo NIPPON MEDICAL SCHOOL, DEPARTMENT OF ANATOMY I, PROFESSOR, 解剖学第一講座, 教授 (70022796)
SUGIMOTO Keiji NIPPON MEDICAL SCHOOL, DEPARTMENT OF ANATOMY I, ASSOCIATE PROFESSOR, 解剖学第一講座, 助教授 (20104002)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | MECHANICAL STRESS / STRESS FIBER / ACTIN / BIOMECHANICS / MOLECULAR AND CELLULAR BIOLOGY / PATENT / CYCLIC STRAIN / EXERCISE / 転写 |
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| Research Abstract |
The stress fiber is one of the most prominent cytoskeletal components that contribute to the maintenance of cell architecture. It has generally been believed that upon cyclic stretching, both cells and their stress fibers become perpendicularly aligned to the direction of stretching. However, using our newly developed stretching device, we have recently found the contrary evidence that stress fibers in endothelial cells rapidly become rearranged at a specific oblique angle relative to the direction of stretching. In light of this finding, we attempted to establish the explanation for such a phenomenon either experimentally or theoretically. It was thus revealed that stress fibers align at a particular angle so as to minimize their length alterations in cyclic deforming fields. Rearrangement of the stress fibers at this angle probably occurs as a result of avoiding compressive stress and may be interpreted as a way of reducing the mechanical stress to which. they are subjected during the deformation. This hypothesis explains well the reason not only for the survival of the stress fibers at a particular oblique angle, but also for the reduced numbers of stress fibers found, at the other angles on cyclic deforming fields. Moreover, theoretical study supports this hypothesis of intracellular stress fiber orientation under cyclic deformation.
We also expand our study from microscopic level to macroscopic level, that is, individual human being. Exercise with skeletal muscle contraction can be considered to mechanical stress from the standpoint of cells in body. We studied cellular response to both endurance and resistance exercise.
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Report
(3 results)
Research Products
(20 results)
