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ANALYSIS TRP TYPE Ca^<2+> CHANNEL COVALENTLY ATTACHED WITH KINASE DOMAIN

Research Project

Project/Area Number 12670039
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

MATSUSHITA Masyuki  Graduate School of Medicine and Dentistry, Okayama University, Assistant, 大学院・医歯学総合研究科, 助手 (30273965)

Co-Investigator(Kenkyū-buntansha) MATSUI Hideki  Graduate School of Medicine and Dentistry, Okayama University, Professor, 大学院・医歯学総合研究科, 教授 (30157234)
MORIWAKI Akiyoshi  Graduate School of Medicine and Dentistry, Okayama University, Assistant Professor, 大学院・医歯学総合研究科, 助教授 (10144742)
TOMIZAWA Kazuhito  Graduate School of Medicine and Dentistry, Okayama University, Lecturer, 大学院・医歯学総合研究科, 講師 (40274287)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsChaK / Trp channel / tongue / Calcium / Kinase / Zinc finger / Crystal Structurre / ATP / Chak / Zinc finger
Research Abstract

A search of EST data bases indicated that there were a number of identified cDNA sequences that were related to the catalytic domain of EF2K. These included genes for EF2K and myosin heavy chain kinase (MHCK) from several species. One novel gene was identified that was distinct from EF2K and MHCK, and this was cloned by library screening from mouse embryo. The full length of this clone was sequenced and found 200kDa protein, designated Channel Kinase (ChaK), which has homology to the TRP channel and kinase domain of EF2K family. The Catalytic domain of Chak was expressed in E.coli and found also to be a Ser/Thr protein kinase. The results obtained suggest that EF2K and ChaK are members of a family of protein kinases that is distinct from the classical protein kinases. The structure of the kinase domain reveals unexpected similality to eukaryotic protein kinases in the catalytic core as well as to metabolic enzymes with ATP grasp domains.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Matsushita, M., Tomizawa, K.et al.: "A high efficiency protein transduction system proving the role of PKA in long lasting LTP"J.Neurosci.. 21(16). 6000-6007 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nairn, AC., Matsushita, M.et al.: "Elongation factor -2 phosphorylation and the regulation of protein synthesis by calcium"Prog.Mol.Subcell.Biol.. 27. 91-129 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamaguchi, H., Matsushita, M.et al.: "Crystal structure of the atypical protein kinase domain of a TRP channel with phosphotransferase activity"Mol.Cell.. 7. 1047-1057 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Chen, Y, Matsushita, M. et al.: "Mechanisms for increased levels of phosphorylation of elongation factor-2 during hibernation in ground squirrels"Biochemistry. 40. 11565-11570 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tomizawa, K., Ohta, J.et al.: "Cdk5/P^<35> regulates neurotransmitter release through phosphorylation and downregulation of P/Q-type voltage-dependent calcium channel activity"J.Neurosci.. (in-press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 松下正之, 富澤一仁 他: "脳の科学"星和書店. 3 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Cheh Y, Matsushita M, Nairn AC, Damuni Z, Cai D, Frerichs KU, Hallenbeck JM.: "Mechanisms tor Increased Levels of Phosphorylation of Elongation Factor-2 during Hibernation in Ground Squirrels."Biochemistry. 40. 11565-11570 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsushita M, Tomizawa K, Moriwaki A, Li SH, H, Terada H and Matsui H.: "A High Efficiency Protein Transduction System Demonstrating the Role of PKA in Long Lasting LTP."J. Neurosci.. 21. 6000-6007 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamaguchi H, Matsushita M, Nairn AC and Kuriyan J: "Crystal Structure of the Atypical Protein Kinase Domain of TRP Channel with Phosphotransferase Activity."Mol Cell.. 7. 1047-1057 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Cho SI, Koketsu M, Fukazawa H, Ishihara H, Matsushita M, Nairn AC, and Uehara Y: "Novel compounds, '1,3-selenazine derivatives' as specific inhibitors of eukaryotic elongation factor 2-kinase (calmodulin-dependent protein kinase-III)"B.B.A.. 1475. 207-215 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsushita, M., Tomizawa, K.et al.: "A high efficiency protein transduction system proving the role of PKA in long lasting LTP"J.Neurosci.. 21(16). 6000-6007 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nairn, AC., Matsushita, M.et al.: "Elongation factor -2 phosphorylation and the regulation of protein synthesis by calcium"Prog.Mol.Subcell.Biol.. 27. 91-129 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamaguchi, H., Matsushita, M.et al.: "Crystal structure of the atypical protein kinase domain of a TRP channel with phosphotransferase activity"Mol.Cell.. 7. 1047-1057 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Chen, Y., Matsushita, M.et al.: "Mechanisms for increased levels of phosphorylation of elongation factor-2 during hibernation in ground squirrels"Biochemistry. 40. 11565-11570 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomizawa, K., Ohta, J.et al.: "Cdk5/P^<35> regulates neurotransmitter release through phosphorylation and downregulation of P/Q-type voltage-dependent calcium channel activity"J.Neurosci.. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 松下正之, 富澤一仁 他: "脳の科学"星和書店. 3 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tomizawa K,Matsushita M. et al.: "Involvement of Cyclin-Dependent Kinase 5/p35(nck5a) in the Synaptic Reorganization of Rat Hippocampus during Kindling Progression. Jpn J Physiol."Jpn.J.Physiol.. 50・5. 525-532 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sung Ig Cho,Mamoru Koketsu, et al.: "Novel compounds,'1,3-selenazine derivatives' as specific inhibitors of eukaryotic elongation factor 2-kinase (calmodulin-dependent protein kinase-III)"B.B.A.. 1475. 207-215 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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