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The research of the regulation of transcription in histidine decarboxylase gene

Research Project

Project/Area Number 12670078
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionTohoku University

Principal Investigator

OHTSU Hiroshi  Tohoku University, Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (60250742)

Co-Investigator(Kenkyū-buntansha) KURAMASU Atsuo  Tohoku University, Medicine, Research Associate, 大学院・医学系研究科, 助手 (90302091)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordshistamine / transcription / histidine decarboxylase / methylation / mast cells / transcription factor / promoter / demethylation
Research Abstract

We have been investigating the transcriptional regulation of a gene coding histidine decarboxylase, the enzyme catalyze histamine synthesis reaction. The expression of this gene is restricted to the mast cells, basophils, neuron, gastric wall etc. The cell specific expression is controlled the methylation of the cytosine of promoter-enhancer region of this gene. We studied this phenomenon in chiefly a cell line called P815 which is a mast cell line. The following statement is the summary of the results.
1) We cloned the mouse genomic DNA of HDC gene and determined the structure.
2) We found that when we treated this cell line with an demethylation agent called 5-azaC, the cell newly expressed HDC gene.
3) We transfected this cell line with two kinds of HDC promoter-reporter plasmids ; a plasmid without any methylation and with methylation in the HDC promoter region. The reporter activity was much weaker in the cells with methylated plasmid.
4) The P815 cell line with methylated promoter-reporter construct increased the reporter activity after the stimulation with 5-aza C.
The mouse HDC gene is controlled by the methylation of the promoter region which is very similar phenomenon seen in human counterparts.
We constructed HDC promoter-GFP vector to check the expression of HDC gene by introducing this gene to generate the transgenic mice. These mice can be used for the transcription assay of HDC by various stimuli and the elucidation of the relation between the inducer and the transcriptional activation in vivo.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Satsuki Suzuki, Ishigaki et al.: "The mouse L-histidine decarboxylase gene shouter and transcriptional rognlation by CpG metheytation in the prosecutor region"Nucleic Acids Research. 28(14). 2627-2633 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ajoy Kumer Ghosh et al.: "Defective angiogenesis in the inflampmatios granbition tissue in histidine decoylepxylass-deficintuiu not mast ull-ddcient mice"J. Exp. Med.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka, S. et al.: "Gastric acid secretion in L-histiding docarbexylace oldicicot mice"Gastroenterology. 122(1). 145-155 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohtsu, H. et al.: "Mice lacking histidine decarboxylase exhitnt alvionnal mast cells"FEBS Letters. 502. 53-56 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuramasu, A. et al.: "Identification of novel mast cell genies by serial analysis of gene expression in cord blood-deviled mast cells"FEBS Letters. 498. 37-41 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hegyi, K. et al.: "Histamine deficiency suppresses unuririe haptoglobin production and modifies hepatic protein tyrosine picosplgorylation"Cell Mol Life Sci. 58. 850-854 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ajoy Kumar Ghosh, et al: "Defective angiogenesis in the inflammation granulation tissue in histidine decarboxylase-deficient mice but not mast cell-deficient mice"J. Exp. Med.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka, S, et al.: "Gastric acid secretion in L-histidine decarboxylase deficient mice"Gastroenterology. 122 (1). 145-155 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohtsu, H., et al.: "Mice lacking histidine decarboxylase exhibit abnormal mast cells"FEBS Letters. 502. 53-56 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kuramasu, A. et al.: "Identification of novel mast cell genes by serial analysis of gene expression in cord blood-derived mast cells"FEBS Letters. 498. 37-41 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hegyi, K. et al.: "Histamine deficiency suppresses murine haptoglobin production and modifies hepatic protein tyrosine phosphorylation"Cell Mol Life Sci. 58. 850-4 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kimura, N. et al.: "Synaptotagmin I expression in mast cells of normal human tissues, systemic mast cell disease, and a human mast cell leukemia cell line"J. Histochem. Cytochem.. 49. 341-345 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Pallinger, E. et al.: "Characterization by flow cytometry of hemopoietic progenitors in bone marrow of histidine decarboxylase knock out and wild type mice"Inflamm Res. S2. S89-S90 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wiener, Z. et al.: "Highly reduced peritoneal mast cell number and decreased c-kit expression in histidine decarboxylase knock out mice"Inflamm Res. S12. S55-6 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ahn, K. et al.: "Regulation of chymase production in human mast cell progenitors"J. Allergy Clin. Immunol.. 106. 321-328 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki-Ishigaki, S et al.: "The mouse 1-histidine decarboxylase gene : structure and transcriptional regulation by CpG methylation in the promoter region"Nucleic Acids Res.. 28 (14). 2627-2633 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Satsuki Suzuki-Ishigaki et al.: "The mouse L-histidine decarboxylase gene : structure and transcriptional regulation by CpG methylation in the promoter region"Nucleic Acids Research. 28(14). 2627-2633 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ajoy Kumar Ghosh et al.: "Defective angiogenesis in the inflammation granulation tissue in histidine decarbosylase-deficient mice but not mast cell-deficient mice"J.Exp.Med.(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tanaka, S. et al.: "Gastric acid secretion in L-histidine decarbosylase deficient mice"Gastroenterology. 122(1). 145-155 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ohtus, H. et al.: "Mice lacking histidine decarboxylase exhibit abnormal mast cells"FEBS Letters. 502. 53-56 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kuramasu, A. et al.: "Identification of novel mast cell genes by serial analysis of gene expression in cord blood-derived mast cells"FEBS Letters. 498. 37-41 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hegyi, K. et al.: "Histamine deficiency suppresses murine haptoglobin production and modifies hepatic protein tyrosine phosphorylation"Cell Mol Life Sci. 58. 850-854 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Satsuki Suzuki-Ishigaki et al.: "The mouse L-histidine decarboxylase gene : structure and transcriptional regulation by CpG methylation in the promoter region"Nucleic Acids Research. 28(14). 2627-2633 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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