| Project/Area Number |
12670110
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Gifu University |
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Principal Investigator |
YOSHIOKA Takashi School of Medicine, Gifu University, Research Associate, 医学部, 助手 (20311699)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Masashi School of Medicine, Gifu University, Research Associate, 医学部, 助手 (40260575)
OKANO Yukio School of Medicine, Gifu University, Professor, 医学部, 教授 (10177066)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Aurora / centrosome / cell division / histone H3 / phosphorylation / NEK / cell division / phosphorvlation / Aurora(AIK) / dominant negative / cell cycle / bladder cancer / colorectal cancer |
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| Research Abstract |
Aurora family kinases are considered to play important roles in chromosome segregation and cytokinesis, consisting of at least 3 members in human. Following new findings were obtained in this study.
(1) Multi-nucleated cells and multi-centrosome cells were observed in cells overexpressing dominant negative Aurora A. Abnormal positionings of centrosome and chromosomes were observed during cell division. Experiments with various deletion mutants of GFP-Aurora A revealed that N-terminal portion of the kinase domain is essential for centrosome localization of Aurora A.
(2) Spatio-temporal localization of Aurora A and B were precisely examined and were found to phosphorylate Ser10 of histone H3 both in vivo and in vitro. Aurora A phosphorylated histone H3 more efficently than Aurora B.
(3) Human NEK7 cDNA, encoding a member of NIMA-related kinases (NEKs) playing important roles in cell division, was cloned and the gene was mapped in Iq31.3. Human NEK3 cDNA was also cloned and mapped in chromosome.
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