| Project/Area Number |
12670126
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MAEKAWA Toshio RIKEN, Molecular Genetics Laboratory, Senior Scientist, 分子遺伝学研究室, 副主任研究員 (90201764)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Transcription factor / ATF-2 / Mammary tumour / GADD45 / APC / c-jun / Anisomycin / Knockoutmouse / ATF2 |
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| Research Abstract |
We have generated and anaiyzed the mutant mouse lacking transcription factor ATF-2 gene far members.
1.ATF-2 heterozygously knockout female mouse developed mammary tumours at very high ratio 18/ : Most of these tumours were adenocarcinoma scirrhus.
2, By using Tip-Technology, we have found that the expressions of APC and c-jun are reduced sev folds in ATF-2 deficient MEF.
3, We have found that the induction of GADD45 gene by anisomycin is inhibited in the ATF-2 defic cell line.
4, The expression levels of GADD45 gene were much reduced in the tumours than in normal mammal
5, ATF-2 homozygously and heterozygously knockout MEFs that express activated RAS develo tumours in nude-mouse at 100%, but wild type MEF that express activated RAS couldn't develop tumours.
6, From these results we can say that ATF-2 heterozygously knockout femaie mouse have high potent to develop tumours.
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