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Search for transcriptional factor which regulates helper T cell Th1/Th2 balance.

Research Project

Project/Area Number 12670140
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKochi Medical School

Principal Investigator

TANIGUCHI Taketoshi  Kochi Medical School, Medicine, Associate prof., 医学部, 助教授 (90127944)

Co-Investigator(Kenkyū-buntansha) FUKUSHIMA Atsuki  Kochi Medical School, Medicine, Reseach Associate, 医学部・附属病院, 助手 (40281737)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsHelper T cells / MHC class II / phorbol ester (TPA) / IFN-γ / Induction of Th1 and Th2 / Lewis, Brown Norway rats / RT-PCR / cytokines / INFγレセプター / ルイス・プラウンノルウェーラット
Research Abstract

Lewis rats are prone to T helper (Th)1 immune responses, whereas Brown Norway (BN) rats are susceptible to Th2 immune responses. Yet, the precise mechanism of induction of the different outcome between these two strains remained elusive. We investigated the expression levels of some cytokines, their receptors and accessory molecules, responsible for the polarization of antigen-specific immune response into a predominant Th1 or Th2 profile in Lewis and BN rats. We observed clear differences between these strains in the expression of IL-12p40, which was high in lymph node cells of Lewis rats. Upregulation of the expression of IL-12 receptor β1, β2, IFN-γ receptor α and β genes were more prominent in Lewis rats rather than BN rats. Thus, IL-12 production by macrophages or dendritic cells seems to be essential event for the activation of Th1 cells
To understand the mechanism of Th1 activaion in Lewis rats, we investigates process of antigen-presentation. Human monocytic leukemia THP-1 cells differentiate into macrophage-like cells when treated with 12-O-tetradecanoyl phorbol-13-acetate (TPA). During this process. IFN-γ-inducible expression of HLA-DRα is markedly enhanced. The enhancement of HLA-DRα expression is at least due to the TPA-dependent induction of the IFN-γ receptor genes. We have studied the mechanism of TPA-induced up-regulation of the IFN-γ R1 gene and identified TPA-responsive element in the promoter of IFN-γ receptor gene.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Sakamoto S, Taniguchi T: "Identification of a phorbol ester-responsive element in the interferongamma receptor 1 chain gene"Journal of Biological Chemistry. 276. 37237-37241 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto S, et al.: "Mechanism for maintenance of dominant T helper 1 immune responses in Lewis rats"Microbiol Immunol.. 45. 373-381 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Song D., Sakamoto S., Taniguchi T.: "Inhibition of Poly(ADP-ribose) Polymerase Activity by Bcl-2 in Association with the ribosomal Protein S3a"Biochemistry. (In press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto S. et al.: "Molecular characterization and nuclear localization of rat timeless-like gene product"Biochem Biophys Res Commun.. 279. 131-138 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto S, Nie J, Taniguchi T: "Phorbol Ester Induction of IFNγReceptors Leads to Enhanced Dr α Gene Expression"Journal of Immunology. 162. 4381-4384 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto S and Taniguchi T.: "Identification of a phorbol ester-responsive element in the interferon-gamma receptor 1 chain gene"Journal of Biological Chemistry. 276. 37237-37241 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto S, et al.: "Mechanism for maintenance of dominant T helper 1 immune responses in Lewis rats"Microbiol Immunol.. 45. 373-381 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Song D., Sakamoto S., and Taniguchi T.: "Inhibition of Poly(ADP-ribose) Polymerase Activity by Bcl-2 in Association with the ribosomal Protein S3a"Biochemistry. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto S. et al: "Molecular characterization and nuclear localization of rat timeless-like gene product"Biochem Biophys Res Commun.. 279. 131-138 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto S, Nie J and Taniguchi T.: "Phorbol Ester Induction of IFNγReceptors Leads to Enhanced Dr α Gene Expression"Journal of Immunology. 162. 4381-4384 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto S, Taniguchi T.: "Identification of a phorbol ester-responsive element in the interferon-gamma receptor 1 chain gene"Journal of Biological Chemistry. 276. 37237-37241 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sakamoto S, et al.: "Mechanism for maintenance of dominant T helper 1 immune responses in Lewis rats"Microbiol Immunol.. 45. 373-381 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Song D., Sakamoto S., Taniguchi T.: "Inhibition of Poly(ADP-ribose) Polymerase Activity by Bcl-2 in Association with the ribosomal Protein S3a"Biochemistry. (in press). (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sakamoto S et.al: "Molecular characterization and nuclear localization of rat timeless-like gene product"Biochem Biophys Res Commun. 279. 131-138 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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