| Project/Area Number |
12670161
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
OKA Takashi Grad. Sch. Med. Dent, Dept of Pathol., Assistant Prof., 大学院・医歯学総合研究科, 助手 (50160651)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHINO Tadashi Grad. Sch. Med. Dent, Dept of Pathol., Lecturor, 大学院・医歯学総合研究科, 講師 (70183704)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | cDNA microarra / Tissue microarray / SHP1 / lymphoma / leukernia / methylation / LOH / human genome / cDNAマイクロアレイ / 病理組織マイクロアレイ / Tリンパ腫 / メチル化 / ヒトゲノム |
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| Research Abstract |
Using cDNA expression array and tissue microarray, we comprehensively and systematically analyzed the expression profile to investigate the lymphomagenesis. We detected the significant decrease of hematopoietic cell specific protein-tyrosine phosphatase SHP1 gene expression in various malignant lymphomas and leukemias. High-frequent silencing of the SHP1 gene by promoter methylation was detected in various kinds of leukemias and lymphomas as well as in many hematopoietic cell lines. Tne promoter methylation of the SHP1 gene was significantly correlated with the clinical stage. Loss of heterozygosiry with microsatellite markers near the SHP1 gene was showed in 79% of informative ALL cases. These results suggest that functional loss of SHP1 is closely associated with the pathogenesis of leukemias/lymphomas
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