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Gene Expression Profile, of Gastric Carcinoma by cDNA Microarray and Laser microdissection

Research Project

Project/Area Number 12670166
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

YOSHIKAWA Yasuji  Medical Institute of Bioregulation Kyushu Univ. Associate Professor, 生体防御医学研究所, 助教授 (80124816)

Co-Investigator(Kenkyū-buntansha) MORI Masaki  Medical Institute of Bioregulation Kyushu Univ. Professor, 生体防御医学研究所, 教授 (70190999)
INOUE Hiroshi  Medical Institute of Bioregulation Kyushu Univ. Associate Professor, 生体防御医学研究所, 助教授 (90203249)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordslaser microdissection / cDNA microarray / gastric cancer / metastatic cancer / primary cancer / T7-based amplification / DNAマイクロアレイ / LCM / Cancer Chip / 癌の悪性度 / 原発巣 / 転移巣 / T7 poluymerase
Research Abstract

Background: The tumor tissue consists of a variable mixture of tumor and host cell populations. Recent development of laser microdissection (LMD) and cDNA microarray analysis urged us to study the differential gene expression profiles among normal cells, primary carcinoma cells, and metastatic carcinoma cells in cases of gastric carcinoma.
Materials and Methods: Total RNA was extracted from the cells obtained by LMD from the primary carcinoma, the corresponding gastric epithelium and the lymph node metastasis in 5 cases of primary gastric carcinoma. RNA was amplified by the T7 based amplification system to apply for the cDNA microarray. The differentially expressed genes among the three populations were evaluated.
Results: cDNA samples for microarray studies were successfully obtained from each cell population of 5 cases.cDNA microarray demonstrated that several interesting genes such as cell cycle regulators and growth factors were overexpressed in metastatic cells compared to primary carcinoma cells. Oncogenes and cell adhesion molecules were overexpressed in primary carcinoma cells compared to normal cells. On the other hand TGF_1 and cadherin were suppressed in primary carcinoma cells compared to normal cells. Interestingly, among MMP family, only MMP7 was picked up as a differentially overexpressed gene in primary carcinoma and metastatic cells compared to normal cells.
Conclusions: This study demonstrated that the combined use of LMD, T7 based amplification and cDNA microarray was able to disclose genes directly associated to each population of tumor tissue. The method will open up new window for precise gene analysis in tumor progression and metastasis.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Mori, M. et al.: "Analysis of the gene-expression profile regarding the progression of human gastric carcinoma"Surgery. 131. S39-S42 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagashima, H. et al.: "Expression of Fas ligand in gastric carcinoma relates to lymph node metastasis"Int J Oncol. 18. 1157-1162 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Etoh, T. et al.: "Increased expression of collagenase-3 (MMP-13) and MT1-MMP in oesophageal cancer is related to cancer aggressiveness"Gut. 47. 56-57 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H Kato, et al.: "Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of the DCC gene"Be J Cancer. 82. 459-466 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 森大輔, 吉河康二: "CSA法によるHPV-DNAの検出の試み"日本臨床細胞学会大分県支部会誌. 11. 16-19 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 吉河康二: "クリプトスポリジウム症の孤発例"大分県医学会雑誌. 19. 1-2 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 吉河康二: "疾病の成り立ちと回復"病理学 廣川書店,東京. 9 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Kato, Y. Zhou, K. Asanoma, H. Kondo, Y. Yoshikawa, K. Watanabe, T. Matsuda, N. WaKe, JC. Barrett: "Suppressed tumorigenicitv of human endometrial cancer cells by the restored expression of the DCC gene"Br. J. Cancer. 82. 459-466 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Nagashima, M. Mori, N. Sadamaga, K. Mashino, Y. Yoshikawa, K. Sigimachi: "Expression of fas ligand in gastric carcinoma relates to lymph node metastasis"Int. J. Oncol.. 18. 1157-1162 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Mori, K. Mimori, Y. Yoshikawa, K. Shibuta, T. Utsunomiya, N. Sadamaga, F. Tanaka, A. Matsuyama, H. Inoue, K. Sigimachi: "Analysis of the gene-expression profile regarding the progression of human gasric carcinoma"Surgery. 131. S39-S47 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kenji Hirayama, Satoshi Shiokawa, Yoshitaka Miyazaki, Michihiro Nakamura, Seiichi Motomura, Youko Suehiro, Yasuji Yoshikawa, Shoichiro Ikuyama, Junji Nishimura: "Primary Sjogren's syndrome complicated by sarcoidosis and psoriasis vulgaris"Mod. Rheumatol.. 11. 356-359 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mori, M. et al.: "Analysis of the gene-expression profile regarding the progression of human gastric carcinoma"Surgery. 131. S39-S42 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nagashima, H. et al.: "Expression of Fas ligand in gastric carcinoma relates to lymph node metastasis"Int J Oncol. 18. 1157-1162 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Etoh, T. et al.: "Increased expression of collagenase-3 (MMP-13) and MT1-MMP in oesophageal cancer is related to cancer aggressiveness"Gut. 47. 50-56 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] H Kato, et al.: "Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of the DCC gene"Br J Cancer. 82. 459-466 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 森大輔, 吉河康二: "CSA法によるHPV-DNAの検出の試み"日本臨床細胞学会大分県支部会誌. 11. 16-19 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 吉河康二: "クリプトスポリジウム症の弧発例"大分県医学会雑誌. 19. 1-2 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 吉河康二: "疾病の成り立ちと回復"病理学 廣川書店, 東京. 9 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] H Kato et al: "Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of the DCC gene."Br J Cancer. 82・2. 459-466 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kataoka A et al: "RT-PCR detection of breast cancer cells in sentinel lymph modes."Int J Oncol. 16. 1147-1152 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mori M et al: "Prognostic impact of tissue inhibitor of matrix metalloproteinase-1 in esophageal carcinoma [In Process Citation]."Int J Cancer. 88. 575-578 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mori M et al: "Altered expression of Fhit in carcinoma and precarcinomatous lesions of the esophagus."Cancer Res. 60. 1177-1182 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamashita K et al: "Clinical significance of matrix metalloproteinase-7 expression in esophageal carcinoma."Clin Cancer Res. 6. 1169-1174 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 吉河康二 他: "クリプトスポリジウム症の単発例"病院病理. 17・1. 5 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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