| Project/Area Number |
12670237
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TEGOSHI Tatsuya Medicine, Kyoto Prefectural University of Medicine Assistant Professor, 医学部, 助手 (40254370)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIKAWA Ryuichi Medicine, Kyoto Prefectural University of Medicine Assistant Professor, 医学部, 助手 (80145466)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | mast sell / Cyto adherence molecule / Nematode infection / E-cadherin / Integrin αE / 培養 / カドヘリン / CD103 |
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| Research Abstract |
Mast cells localize in various tissues through adhesive interactions with tissue components. Cell adhesion molecules are thought to play a critical role in cell migration and localization within tissues including gut mucosa. We found the expression of E-cadherin and cadherin-associated cytoplasmic proteins such as α-, β-, p120-catenin in bone marrow-derived mast cells (BMMC) cultured in interleukin-3 (IL-3) and IL-4 rich medium. E-cadherin was associated with the regulation of certain stages of mast cells differentiation and cell-cell interaction confirmed by colony assay and cell adhesion assay. Next, to clarify the expression of gut intraepithelium-related cell adhesion molecules such as E-cadherin and integrin αE (CD103)β7, BMMC cultured in the growth medium containing with IL-3, IL-9, stem cell factor (SCF), and transforming growth factor-β1 (TGF-β1 were used an in vitro analog of intestinal mucosal mast cells (IMMC). Mouse mast cell protease-1 (mMCP-1) expressed on only IMMC was induced in BMMC supplemented with these growth factors. Significant expression of integrin αE, and β7 subunit were observed in cultured mucosal type mast cells. E-cadherin was also expressed on these mast cells.
These results suggest that E-cadherin and its heteroligand integrin αEβ7 expressed on mast cells might play a role in mast cell-epithelial cell adhesion and/or remaining anchored at the intestinal epithelia by heterotypic or homotypic interacting with E-cadherin.
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