| Project/Area Number |
12670243
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
寄生虫学(含医用動物学)
|
|---|
| Research Institution | Aichi Medical University School of Medicine |
|---|
Principal Investigator |
ITOH Makoto Aichi Medical University School of Medicine, Department of Medicine, Associate Professor, 医学部, 助教授 (90137117)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KIMURA Eisaku Aichi Medical University School of Medicine, Department of Medicine, Professor, 医学部, 教授 (70153187)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
|---|
| Keywords | filariasis / diagnosis / ELISA / urine / epidemiology |
|---|
| Research Abstract |
Development and standardization of a diagnostic method using urine samples to find out endemic foci and to evaluate control programs, which is essential for a global program to eliminate lymphatic filariasis' launched by WHO, is the main purpose of this study.
1. Development of diagnostic method with urine samples for bancroftian filariasis
We developed an ELISA method using urine samples for bancroftian filariasis, which detect antigen specific IgG4 in urine. Both the sensitivity and specificity of the method were high; 96% and 99%, respectively. Little diurnal fluctuation of antibody titers and preservability of the urine samples after addition of sodium azide are advantage of this method for application to field studies.
2. Application of the urine-ELISA to find out endemic foci in Sri Lanka
The urine ELISA was used to find out endemic foci in a mountainous area in Sri Lanka where no information on filariasis was available. We examined 2,500 urine samples and found positives (4%). This
… More
positive rate was higher than the rate obtained by antigen detection kit with serum samples (0.6%) showing that the urine-ELISA is useful for finding endemic foci.
3. Application of the urine-ELISA to evaluate a control program in China
The urine-ELISA was used to evaluate a control program carried out in Yongjia in Zhejiang Province where no microfilaria positives has not been found for 10 years. All the samples collected from school age children, 2,800 samples, were urine-ELISA negative. Measurement of antigen specific IgG4 in urine samples from young generation with this method is considered a useful means for evaluation of filariasis control.
4. Application of the urine-ELISA to another lymphatic filariasis, malayan filariasis
The urine-ELISA was used to detect antigen specific IgG4 in urine samples from residents in Brugia malayi endemic Islands in Korea. All microfilaria positives were urine-ELISA positive. All urine samples from young generation were negative, showing no recent transmission. Basic study to show sensitivity of the method for malayan filariasis is under study. Less
|
