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Trial of recombinant MSP-1 vaccine combined with T lymphocyte triggering factor(TLTF)

Research Project

Project/Area Number 12670244
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionKurume University

Principal Investigator

FUKUMA Toshihide  Univ. Sch. Med., Dept. Parasitol., Professor., 医学部, 教授 (90125146)

Co-Investigator(Kenkyū-buntansha) HARA Tatsuru  Kurume Univ. Sch. Med., Dept. Parasitol., Research Associate, 医学部, 助手 (30238159)
HIRATA Mizuki  Kurume Univ. Sch. Med., Dept. Parasitol., Associate Professor., 医学部, 助教授 (70080629)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsmalaria / vaccine / MSP-1 / Trypanosoma brucei / VSG / TLTF
Research Abstract

We examined the basic research of malaria vaccicne development using African trypanosome (Trypanosoma brucei). Outlines of the results are as follows.
1 : The trypanosome expression vectors were constructed with MSP-1 gene (msp-1). These vectors were transfected into T. brucei procyclic form, and expressions were analysed. However, we could not confirm the expression of MSP-1.It is suggested that msp-1 or MSP-1 may have some responsibility in molecular folding or conformation, for example, because the drug resistance gene included in the vectors were functioned and the other reporter genes constructed into the vectors were expressed as expected.
Then, the policy of this project was converted to malaria vaccine development using the adjuvant effect of variable surface glycoproteins (VSG) of the T brucei bloodstream form.
2 : The fusion proteins of [Histidine TagIVSG N-terminal conserved domain/MSP-1/VSG C-terminal conserved domain] (VSG-MSP) and [Histidine Tag/MSP-1] were tried to express using vaculovirusinsect cell system. The recombinant fusion proteins were solubilized with guanidine, and were purified using the Ni-agarose affinity column.
3 : Mice were immunized by subcutaneous injection of recombinant proteins and were subsequently boosted 3 weeks and 6 weeks after the initial immunization. Three weeks after the final inoculation, all mice were challenged with the parasitized 106 erythrocytes by intraperitoneal injection. As the result, slight preventive effects on 9arasitemia were obserbed when mie were immunized with VSGMSP, though they could not survived the infection.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kisaburo Nagamune, et al.: "Critical roles of glycosylphosphatidylinositol for Trypanosoma brucei"Proc.Natl.Acad.Sci.U.S.A.. 97. 10336-10341 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tatsuru Hara, et al.: "Effective gene transfer into Trypanosoma brucei bloodstream forms by particle bombardment"Mol.Biochem.Parasitol.. 119. 117-119 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Elvis Nyarko, et al.: "Toxic effects of mercury(II), cadmium(II) and lead(II) porphyrins on Trypanosoma brucei brucei growth"Chemico-Biological Interactions. 139. 177-185 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Toshihide Fukuma: "Molecular biology of the trypanosomes. In Progress of Medical Parasitology in Japan"Meguro Parasitological Museum, Tokyo(in pree). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kisaburo Nagamune, Tomoyoshi Nozaki, Yusuke Maeda, Kazuhito Ohishi, Toshihide Fukuma, Tatsuru Hara, Ralph T. Schwarz, Christine Siitterlin, Reto Brun, Howard Riezman, Taroh Kinoshita: "Critical roles of glycosyIphosphatidylinositol for Trypanosoma brucei."97. 10336-10341 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tatsuru Kara, Kouichi Yasuda, Toshihide Fukuma: "Effective gene transfer into Trypanosoma brucei bloodstream forms by particle bombardment"Molecular and Biochemical Parasitology. 119. 117-119 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Elvis Nyarko, Tatsuru Hara, Dennis J. Grab, Masaaki Tabata, Toshihide Fukuma: "Toxic effects of mercury(II), cadmium(II) and lead(II) porphyrins on Trypanosoma brucei brucei growth."Chemico-Biological Interactions. 139. 177-185 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] ToshihideFukuma: "Molecular biology of the trypanosomes"Meguro Parasitological Museum, Tokyo. 7(in press). 471-490 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Hara: "Effective gene transfer into Trypanosoma brucei bloodstream forms by particle bombardment"Mol.Biochem.Parasitol.. 119. 117-119 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Hirata: "Effect of nitric oxide synthase inhibition on Schistosoma japonicum egg-induced granuloma formation in the mouse liver"Parasite Immunology. 23. 281-289 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Hirata: "Schistosoma japonicum egg granuloma formation in the interleukin -4 or interferon -γ deficient host"Parasite Immunology. 23. 271-280 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Nagamune,T.Nozaki,Y.Maeda,K.Ohishi,T.Fukuma, et al.: "Critical roles of glycosylphosphatidylinositol for Trypanosoma brucei"Proc.Natl.Acad.Sci.USA. 97. 10336-10341 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Sugita, et al.: "Primary amebic meningoencephalitis due to Naegleria fowleri : an autopsy case in Japan."Pathology International. 49. 468-470 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Hirata,T.Nakashima and T.Fukuma: "Characteristics of eosinophilic inclusions within Shistosoma japonicum eggs."Parasitology. 118. 269-274 (1999)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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