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Molecular epidemiology of Clostridium difficile

Research Project

Project/Area Number 12670252
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKanazawa University

Principal Investigator

NAKAMURA Shinichi  Kanazawa Universiy, Graduate School of Medical Science, Professor, 大学院・医学系研究科, 教授 (90019620)

Co-Investigator(Kenkyū-buntansha) MAEGAWA Tuneo  Kanazawa Universiy, Graduate School of Medical Science, Assistant, 大学院・医学系研究科, 助手 (50283114)
KATO Haru  National Institute of Infectious Diseases, Department of Bactrial and Blood Products, Chief Researcher, 細菌・血液製剤部, 主任研究官 (00273136)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsClostridium difficlle / Antibiotic-associated dearrhea / Molecular epidemiology / PCR ribotyping / PFGE analysis / Community transmission / Family transmission / Epidemic strain / 腸管内保有 / 院内感染 / 血清型JP
Research Abstract

1. Moleculay epidemiology of Clostridium difficile on carriage and transmission in community settings and family members
(1) Out of 1,413 individuals, who were composed of nine groups, overall, 108 (7.6%) individuals were positive for C. difficile by faecal culture but carriage rates among the study groups ranged from 4.2% to 15.3%.
(2) Typing by PCR ribotyping and pulsed-field gel electrophoresis (PFGE) demonstrated clusters, of carriers colonised by a single type in each of three groups.
(3) Two or more family members were C. difficile-positive in five of 22 families examined. C. difficile with an identical type was isolated from persons within only one family.
These findings indicate that cross-transmission of C. difficile cam occur in community settings, and that cross-transmission of C. difficile does not occur frequently among family members at home.
2. Molecular analysis of C. difficile from nosocomial outbreaks in Japan
(1) At three hospitals examined, a single PCR ribotype strain (type smz) was predominant and accounted for 22 (65%) of 34, 18 (64%) of 28, and 11 (44%) of 25 isolates, respectively. All of the 51 isolates that represented PCR ribotype smz were nontypeable by PFGE because of DNA degradation.
(2) All 51 type smz isolates belonged to a new serogroup (designated serogroup JP), reacting with the newly prepared antiserum.
(3) The PCR ribotype smz strain was not found among the isolates from healthy adults. These findings indicate that the strain with PCR ribotype smz, PFGE-nontypeabe and serogroup JP is likely to be epidemic in Japan.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report

Research Products

(9 results)

All Other

All Publications (9 results)

  • [Publications] 加藤はる: "PCRリボタイピングおよびパルスフィールド電気泳動タイピングによるClostridium difficile集団発生の解析"日本嫌気性菌感染症研究. 30. 73-77 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Kato: "Analysis of Clostridium difficile isolates from nosocomial outbreaks at three hospitals in diverse areas of Japan"Journal of Clinical Microbiology. 39. 1391-1395 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 加藤はる: "3病院で院内感染を引き起こしたClostridium difficileのタイピングによる解析"日本細菌学雑誌. 56. 325 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Kato: "Colonization and transmission of Clostridium difficile in healthy individuals examined by PCR ribotyping and pulsed-field gel electrophoresis"Journal of Medical Microbiology. 50. 720-727 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Kato: "Analysis of Clostridium difficile isolates from nosocomial outbreaks at three, hospitals in diverse areas of Japan"Journal of Clinical Microbiology. 39. 1391-1395 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Kato: "Colonization and transmission of Clostridium difficile in healthy individuals examined by PCR ribotyping and pulsed-field gel electrophoresis"Journal of Medical Microbiology. 50. 720-727 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Kato: "Colonization and transmission of Clostridium difficile in healthy individuals examined by PCR ribotyping and pulsed-field gel electrophoresis"Journal of Medical Microbiology. 50. 720-727 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Kato: "Analysis of Clostridium difficile isolates from nosocomial outbreaks at three diverse hospitals in Japan"Journal of Clinical Microbiology. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 加藤はる: "PCRリボタイピングおよびパルスフィールド電気泳動タイピングによるClostridium difficile集団発生の解析"日本嫌気性菌感染症研究. 30. 73-77 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-03-31   Modified: 2016-04-21  

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