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New protective factors expressed in phagocytes against infection

Research Project

Project/Area Number 12670271
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

KURA Fumiaki  National Institute of Infectious Diseases, Department of Bacteriology, Senior Research Scientist (30161722)

Co-Investigator(Kenkyū-buntansha) AMEMURA-MAEKAWA Junko  Natianal Institute of Infectious Diseases, Department of Bacteriology, Research Scientist (20238843)
TSUKANO Hiroko  National Institute of Infectious Diseases, Department of Bacteriology ; Chief, Laboratory of Systemic Infection (50072955)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsLegionella / macrophages / natural resistance / microglia
Research Abstract

Two types of response against microbial infections have been known, acquired-resistance through cell-mediated immunity and natural resistance. Legionella pneumophila (Lp), an intracellular bacterium, causes severe pneumonia in compromised humans. In this study, we examined protective factors against Legionella infection using Lgnl-congenic mice, which are different in the capability of macrophages to restrict Lp growth, and knockout mice defective in production of bactericidal active oxygen species. (1) We examined bacterial modulation of host response using immunomagnetic beads with live or heat-killed Lp. The immunomagnetic beads were phagocytosed by peritoneal macrophages from A/J mice (Lgnl^s) in vitro culture. The macrophages were harvested and homogenized. The phagosomes from the macrophages were collected using a magnet and their components were analyzed by electrophoresis and western blotting. A subunit of NADPH oxidase was decreased in the phagosomes with live Lp but not in those with heat-killed Lp, suggesting the possibility that Lp suppresses the integrity and the activity of NADPH oxidase in Lp-harboring phagosomes. (2) Lp burden in lungs was comparable between wild and myeloperoxidase (MPO)-knockout C57B1/6 mice (Lgnl^r) after intranasal infection. However, in Lgnl^s mice of A/J background, MPO-knockout mice revealed more bacterial growth in lungs. In the situation that Lp can multiply in macrophages, neutrophil MPO was suggested to contribute to the protection against Lp infection, in an addition to NADPH oxidase.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (12 results)

All 2002 2001 2000 Other

All Journal Article (7 results) (of which Peer Reviewed: 2 results) Book (1 results) Publications (4 results)

  • [Journal Article] Cross-reactivity and sensitivity of two legionella urinary antigen kits, Biotest EIA and Binax NOW, to extracted antigens from various serogroups of L.pneumophila and other Legionella species.2002

    • Author(s)
      Okada C., Kura F., Wada A., Inagawa H., Lee G.-H., Matsushita H.
    • Journal Title

      Microbiology and Immunology 46

      Pages: 51-54

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Critical role of myeloperoxidase and nicotinamide adenine dinucleotide phosphate-oxidase in high-burden systemic infection of mice with Candida albicans.2002

    • Author(s)
      Aratani Y., Kura F., Watanabe H., Akagawa K., Takano Y., Suzuki K., Dinauer M.C., Maeda N., Koyama H.
    • Journal Title

      Journal of Infectious Diseases(accepted)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Journal Article] Cross-reactivity and sensitivity of two Legionella urinary antigen kits, Biotest EIA and Binax NOW, to extracted antigens from various serogroups of L.pneumophila and other Legionella species2002

    • Author(s)
      Okada C., Kura F., Wada A., Inagawa H., Lee G.-H., Matsushita H.
    • Journal Title

      Microbiology and Immunology 46

      Pages: 51-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Journal Article] Critical role of myeloperoxidase and nicotinamide adenine dinucleotide phosphate-oxidase in high-burden systemic infection of mice with Candida albicans2002

    • Author(s)
      Aratani Y., Kura F, Watanabe H., Akagawa K., Takano Y., Suzuki K., Dinauer M.C., Maeda N., Koyama H.
    • Journal Title

      Journal of Infectious Diseases (accepted)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Journal Article] Analysis of Legionella pneumophila serogroup 1 isolates in Japan by pulsed-field gel electrophoresis and monoclonal antibodies2001

    • Author(s)
      Amemura-Maekawa J., Kura F., Gondaira F., Sugiyama J., Watanabe H.
    • Journal Title

      Legionella(Marre R., et al.(ed.)(ASM Press) 302-304

      Pages: 459-459

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Journal Article] Differential host susceptibility to pulmonary infections with bacteria and fungi in mice deficient in myeloperoxidase2000

    • Author(s)
      Aratani Y., Kura F., Watanabe H., Akagawa K., Yakano Y., Suzuki K., Maeda N., Koyama H.
    • Journal Title

      Journal of Infectious Diseases 182

      Pages: 1276-1279

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Differential host susceptibility to pulmonary infections with bacteria and fungi in mice deficient in myeloperoxidase2000

    • Author(s)
      Aratani Y., Kura F., Watanabe H., Akagawa K., Takano Y., Suzuki K., Maeda N., Koyama H.
    • Journal Title

      Journal of Infectious Diseases 182

      Pages: 1276-1279

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Book] Analysis of Legionella pneumophila serogroup 1 isolates in Japan by pulsed-field gelelectrophoresis and monoclonal antibodies, p302-304. In Marre R., et al.(ed.), Legionella2001

    • Author(s)
      Amemura-Maekawa J., Kura F., Gondaira F., Sugiyama J., Watanabe H.
    • Total Pages
      459
    • Publisher
      ASM Press
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okada, C., Kura, F., Wada, A., Inagawa, H, Lee, G-H., Matsushita, H.: "Cross-reactivity and sensitivity of two Legionella urinary antigen kits, Biotest EIA and Binax Now, to extracted antigens from various serogroups of L.pneumohila and other Legionella species"Microbiology and Immunology. 46. 51-54 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Aratani, Y., Kura, F., Watanabe, H., Akagawa, H., Takano, Y., Suzuki, K., Dinauer, M.C., Maeda, N., Koyama, H: "Critical role of myeloperoxidase and NADPH-oxidase in high-burden systemic infection of mice with Candida albicans"Journal of Infectious Diseases. (accepted). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Amemura-Maekawa J., Kura F., Gondaira F., Sugiyama J., Watanabe H.: "Analysis of Legionella pneumophila serogroup 1 isolates in Japan by pulsed-field gel electrophoresis and monoclonal antibodies. p3O2-3O4. In Marre R., et al. (ed.), Legionella"ASM Press. 459 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 荒山康昭: "Differential Host Susceptibility to Pulmonary Infections with Bacteria and Fungi in Mice Deficient in Myeloperoxidase"Journal of Infectious Diseases. 182・4. 1276-1279 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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