| Project/Area Number |
12670285
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Soka University |
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Principal Investigator |
TAKASE-YODEN Sayaka Soka University, Institute of Life Science, Professor, 生命科学研究所, 教授 (60236221)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUMITSU Hidefumi Soka University, Institute of Life Science, Instructor, 生命科学研究所, 助手 (00308280)
KANAMATSU Tomoyuki Soka University, Institute of Life Science, Professor, 生命科学研究所, 教授 (30104201)
WATANABE Rihito Soka University, Institute of Life Science, Professor, 生命科学研究所, 教授 (30129746)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Retrovirus / Brain / Neuropathogenic / Glial cell / BDNF / NGF / Receptor / ^<13>C-Glucose |
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| Research Abstract |
A neuropathogenic variant of Friend murine leukemia virus (F-MLV) clone A8 induces spongiform degeneration in the central nervous system (CNS) without cell infiltration when inoculated into newborn rats. In the lesions, neuropil vacuolation is observed by electron microscopy. Immunohistochemical studies showed that the viral antigens are mainly found in endothelial cells in the CNS of rats infected with the A8 virus. Glial cells also express the viral antigen, but neurons do not. In this study, we focused on the role of glial cells in spongiform degeneration in rat brains caused by retrovirus infection and obtained the results as follows : 1. Distribution of the lesions in the CNS of A8-infected rats was correlated with that of the glial cells expressing viral antigens rather than the endothelial cells. 2. Expression of the viral receptor on glial cells of brains of 3-week-old rats was lower than that of newborn rats. 3. Measurement of glial metabolism in rat brains by the use of ^<13>C-glucose showed that the glial cells in the brains of rats infected with neuropathogenic F-MLV became hyperactive. 4. The transcripts of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) were detected by relative quantitative RT-PCR in cultured glial cells F10 and C6. Examination of the effects of A8-virus infection on the amount of these mRNA is in progress. These findings suggest that retrovirus receptor-bearing glial cells contribute to the neuropathogenesis of MLV.
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