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Pattern recognition by human γδ T cells and innate immunity.

Research Project

Project/Area Number 12670299
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TANAKA Yoshimasa  Kyoto Univ. Grad. Sch. Biostudies Lab. Immunol. Cell Biol. Lecturer, 生命科学研究科, 助手 (90280700)

Co-Investigator(Kenkyū-buntansha) HATTORI Masakazu  Kyoto Univ. Grad. Sch. Biostudies Lab. Immunol. Cell Biol. Assistant Professor, 生命科学研究科, 助教授 (40211479)
MINATO Nagahiro  Kyoto Univ. Grad. Sch. Biostudies Lab. Immunol. Cell Biol. Professor, 生命科学研究科, 教授 (40137716)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsimmune system / γδ T cell / nonpeptide antigen / T cell receptor / pathogenic microbe / innate immunity / acquired_immunity / tumor cell / ピロリン酸モノエステル / アルキルアミン / 結核菌 / 病原性大腸菌 / パターン抗原 / 非ペプチド抗原 / パターン認識
Research Abstract

Innate immunity and acquired immunity play an essential role in human immune system, in which the former system recognize pathogen-associated molecular patterns and the latter non-self peptide antigens. The representative cells are macrophages and αβ T cells, respectively. Although human γδ T cells are considered to be involved in innate immunity since they recognize pyrophosphomonoesters and alkyl amines derived from pathogenic microbes, they are, on their other hand, classified as a member of acquired immunity based on their cell surface receptor generated as a result of gene rearrangement. In this study, immunological nature of human γδ T cells was elucidated by examining their recognition mechanism of nonpeptide antigens. Nitrogen-containing bisphosphonate was used in the present study. In the primary reaction, cell aggregation and interferon-γ Production were dependent on the existence of macrophage-lineage cells, indicating that antigen-presenting cells are involved in the recognition of nonpeptide antigens by γδ T cells. In addition, tumor cells were demonstrated to be able to present nonpeptide antigens to human γδ T cells in the secondary reaction. In order to elucidate the precise recognition mechanism of nonpeptide antigens by γδ T cell receptors, gene transfer system using a TCR-negative Jurkat cell line was employed, in which germline-encoded lysine residues in Jγ1.2 segment were shown to be involved in the interaction. This indicates that canonical sequence of γδ T cell receptors recognizes directly nonpeptide antigens, while γδ T cell receptors undergo gene rearrangement. Taken, together, human γδ T cells were confirmed to have two distinct characters, innate immunity and acquired immunity, on the basis of their mechanism for antigen recognition.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Fumi Miyagawa, Yoshimasa Tanaka, Seiji Yamashita, Bunzo Mikami, Kiichiro Danno, Masami Uehara, Nagahiro Minato: "Essential contribution of germline-encoded lysine residues in Jγ1.2 segment to the recognition of nonpeptide antigens by human γδ T cells"The Journal of Immunology. 167. 6773-6779 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yu Kato, Yoshimasa Tanaka, Fumi Miyagawa, Seiji Yamashita, Nagahiro Minato: "Targeting of tumor cells for human γδ T cells by nonpeptide antigens"The Journal of Immunology. 167. 5092-5098 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Craig T.Morita, Hoi K.Lee, Hong Wang, Hongmin Li, Roy A.Mariuzza, Yoshimasa Tanaka: "Structural features of nonpeptide prenyl pyrophosphates that determine their antigenicity for human γδ T cells"The Journal of Immunology. 167. 36-41 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fumi Miyagawa, Yoshimasa Tanaka, Seiji Yamashita, Nagahiro Minato: "Essential requirement of antigen presentation by monocyte lineage cells for the activation of primary human γδ T cells by aminobisphosphonate antigen"The Journal of Immunology. 166. 5508-5514 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroyuki Nishimura, Taku Okazaki, Yoshimasa Tanaka, Kazuki Nakatani, Masatake Hara, Akira Matsumori, Shigetake Sasayama, Akira Mizoguchi, Hiroshi Hiai, Nagahiro Minato, Tasuku Honjo: "Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice"Science. 291. 319-322 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Seigo Korematsu, Yoshimasa Tanaka, Susumu Hosoi, Satoshi Koyanagi, Toyokazu Yokota, Bunzo Mikami, Nagahiro Minato: "C8/119S mutation of major mite allergen Derf-2 leads to degenerate secondary structure and molecular polymerization and induces potent and exclusive Th1 cell differentiation"The Journal of Immunology. 165. 2895-2902 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fumi Miyagawa, Yoshimasa Tanaka, Seiji Yamashita, Bunzo Mikami, Kiichiro Danno, Masami Uehara, and Nagahiro Minato: "Essential contribution of germline-encoded lysine residues in Jγ1.2 segment to the recognition of nonpeptide antigens by human γδ T cells"The Journal of Immunology. 167. 6773-6779 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yu Kato, Yoshimasa Tanaka, Fumi Miyagawa, Seiji Yamashita, and Nagahiro Minato: "Targeting of tumor cells for human γδ T cells by nonpeptide antigens"The Journal of Immunology. 167. 5092-5098 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Craig T. Morita, Hoi K. Lee, Hong Wang, Hongmin Li, Roy A. Mariuzza, and Yoshimasa Tanaka: "Structural features of nonpeptide prenyl pyrophosphates that determine their antigenicity for human γδ T cells"The Journal of Immunology. 167. 36-41 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fumi Miyagawa, Yoshimasa Tanaka, Seiji Yamashita, and Nagahiro Minato: "Essential requirement of antigen presentation by monocyte lineage cells for the activation of primary human γδ T cells by aminobisphosphonate antigen"The Journal of Immunology. 166. 5508-5514 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirohito Kobayashi, Yoshimasa Tanaka, Junji Yagi, Hiroshi Toma, and Takehiko Uchiyama: "Gamma/ delta T cells provide innate immunity against renal cell carcinoma"Cancer Immunology and Immunotherapy. 50. 115-124 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroyuki Nishimura, Taku Okazaki, Yoshimasa Tanaka, Kazuki Nakatani, Masatake Hara, Akira, Matsumori, Shigetake Sasayama, Akira Mizoguchi, Hiroshi Hiai, Nagahiro Minato, and Tasuku Honjo: "Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice"Science. 291. 319-322 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Seigo Korematsu, Yoshimasa Tanaka, Susumu Hosoi, Satoshi Koyanagi, Toyokazu Yokota, Bunzo Mikami, and Nagahiro Minato: "C8/119S mutation of major mite allergen Derf-2 leads to degenerate secondary structure and molecular polymerization and induces potent and exclusive Tn1 cell differentiation"The Journal of Immunolgy. 165. 2895-2902 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fumi Miyagawa, Yoshimasa Tanaka, Seiji Yamashita, Bunzo Mikami, Kiichiro Danno, Msami Uehara, Nagahiro Minato: "Essential contribution of germline-encoded lysine residues in Jγ1.2 segment to the recognition of nonpeptide antigens by human γδT cells"The Journal of Immunology. 167. 6773-6779 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yu Kato, Yoshimasa Tanaka, Fumi Miyagawa, Seiji Yamashita, Nagahiro Minato: "Targetin of tumor cells for human γδT cells by nonpeptide antigens"The Journal of Immunology. 167. 5092-5098 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fumi Miyagawa, Yoshimasa Tanaka, Seiji Yamashita, Nagahiro Minato: "Essential requirement of antigen presentation by monocyte lineage cells for the activation of primary human γδT cells by aminobisphosphonate antigen"The Journal of Immunology. 166. 5508-5514 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Craig T.Morita, Hoi K.Lee, Hong Wang, Hongmin Li, Roy A.Mariuzza, Yoshimasa Tanaka: "Structural features of nonpeptide prenyl pyrophosphates that determine their antigenicity for human γδT cells"The Journal of Immunology. 167. 36-41 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirohito Kobayashi, Yoshimasa Tanaka, Junji Yagi, Hiroshi Toma, Takehiko Uchiyama: "Gamma/delta T cells provide innate immunity against renal cell carcinoma"Cancer Immunology and Immunotherapy. 50. 115-124 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Seigo Korematsu: "C8/H9S mutation of major mite allergen Derf-2 leads to degenerate secondary structure and molecular polymerization and induces potent and exclusive Th1 cell differentiation"Journal of Immunology. 165. 2895-2902 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hiroyuki Nishimura: "Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice"Science. 291. 319-322 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fumi Miyagawa: "Essential requirement of antigen presentation by monocyte-leneage cells for the activation of primary human γδT cells by aminobisphosphonate antigen"Journal of Immunology. (In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirohito Kobayashi: "γδT cells provide innate immunity against renal cell carcinoma"Cancer Immunology and Immunotherapy. (In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 是松聖悟: "イエチリダニの抗原構造とTh1/Th2の誘導"臨床免疫. 34・6. 752-760 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 是松聖悟: "アレルギーの新しい減感作"免疫・Immunology Frontier. 10・6. 36-41 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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