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Role ofprostaglandin in the selective activation of Th2 cells in BALB/c mice

Research Project

Project/Area Number 12670311
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

YAMASHITA Uki  UOEH, Sch. Med., Professor, 医学部, 教授 (00028680)

Co-Investigator(Kenkyū-buntansha) SUGIURA Tsutomu  UOEH, Sch. Med., Assistant Prof., 医学部, 講師 (40131924)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsTh1 / Th2 / IFN-y / IL-12 / MDC / prostaglandin / BALB / c mouse / IFN-γ / IL-4
Research Abstract

There are two subsets of helper T cells, Th1 and Th2.Th1 mediates cellular immunity, and Th2 mediates humoral immunity. Generally, in BALB/c mice, Th2 is more easily activated by immunization and infection when compared with C3H/He and C57BL/6 mice, and higher level of serum IgE and eosinophilia are induced. However, the precise mechanism of Th2 activation in BALB/c mice is not fully understood. Prostaglandin E_2 (PGE_2) is reported to have immunosuppressive activity, especially on Th1-mediated immune responses. We studied the role of PGE_2 in the production of cytokines and chemokines by spleen cells and peritoneal exudates cells stimulated with Staphyhcoccus aureus Cowan I (SAC) using BALB/c, C3H/HeN and C57BL/6 mice and obtained the following results. (1)The treatment of spleen cells with indomethacine, cyclooxygenase (COX)-1 and COX-2 inhibitor, or NS-398, COX-2 specific inhibitor, more clearly enhanced IFN-γ-production by spleen cells from BALB/c mice than from C3H/He and CSTBL/c mice. (2)The amount of PGEz after SAC-stimulation is higher in BALB/c mice than C3H/He and C57BL/6 mice. (3)The addition of PGE_2 into the spleen cell culture more clearly suppressed IFN-γ and IL42p70 production by BALB/c spleen cells than C3H/HeN and C57BL spleen cells. (4)The in vivo administration of NS-398 into BALB/c mice enhanced antigen-specific IFN-γ production and its enhancing activity was similar to that of IL-12.(5)Macrophage-derived chemokine (MDC) production is also higher in BALB/c mice, and its production is further upregulated by PGE_2. These results suggest that PGE_2 plays an important role for the preferential activation of Th2 in BALB/c mice by suppressing IL-12p70 production and enhancing MDC production by antigen-presenting cells.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] E.Kuroda, T.Sugiura, K.Okada, K.Zeki, U.Yamashita: "Prostaglandin E_2 up-regulates macrophage-derived chemokine production but suppresses IFN-inducible protein-10 production by APC"J. Immunology. 166. 1650-1658 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] E.Kuroda, Y.Yoshida, B.E.Shan, U.Yamashita: "Suppression of macrophage interleukin-12 and tumour necrosis factor-α production in mice infected with Toxocara canis"Parasite Immunology. 23. 305-311 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Okada, T.Sugiura, E.Kuroda, S.Tsuji, U.Yamashita: "Phenytoin promotes Th2 type immune response in mice"Clinical and Experimental Immunology. 124. 406-413 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Okada, S.Tsuji, U.Yamashita: "Cyclooxygenase-2 expression in the hippocampus from genetically epilepsy susceptible El mice"Brain Research. 894. 332-335 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] E.Kuroda, T.Sugiura, K.Zeki, Y.Yoshida, U.Yamashita: "Sensitivity difference to the suppressive effect of prostaglandin E 2 among mouse strains : A possible mechanism to polarize Th2 type response in BALB/c mice"J. Immunology. 164. 2386-2395 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Okada, Y.Yoshida, T.Sugiura, H.Tanaka, S.Tsuji, H.Yamashita, U.Yamashita: "Reduced interleukin-1 responsiveness in immune system and central nervous system of inbred polydipsic (STR/N) mice"Life Sciences. 99. 1461-1470 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] E.Kuroda, T.Sugiura, K.Okada, K.Zeki, U.Yamashita: "Prostaglandin E_2 up-regulates macrophage-derived cheirtokine production but suppresses IFN-inducible protein- 10 production by APC"J. Immunology. 166. 1650-1658 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] E.Kuroda, Y.Yoshida, Shan B.E., U.Yamashita: "Suppression of macrophagc interleukin-12 and tumour necrosis factor-a production in mice infected with Toxocara canis"Parasite Immunology. 23. 305-311 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Okada, T.Sugiura, E.Kuroda, S.Tsuji, U.Yamashita: "Phenytoin promotes Th2 type immune response in mice"Clinical and Experimental Immunology. 124. 406-413 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Okada, U.Tsuji, U.Yamashita: "Cyclooxygenase-2 expression in the hippocampus from genetically epilepsy susceptible E1 mice"Brain Research. 894. 332-335 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] E.Kuroda, T.Sugiura, K.Zeki, Y.Yoshida, U.Yamashita: "Sensitivity difference to the suppressive effect of prostaglandin E_2 among mouse strains : A possible mechanism to polarize Th2 type response in BALB/c mice"J. Immunology. 164. 2386-2395 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Okada, Y.Yoshida, T.Sugiura, H.Tanaka, S.Tsuji, H.Yamashita, U.Yamashita: "Reduced interleukin-1 responsiveness in immue system and central nervus system of inbred polydipsic(STR/N) mice"Life Sciences. 99. 1461-1470 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] E.Kuroda, T.Sugiura, K.Okada, K.Zeki, U.Yamashita: "Prostaglandin E_2 up-regulates macrophage-derived chemokine production but suppresses IFN-inducible protein-10 production by APC"J. Immunology. 166. 1650-1658 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] E.Kuroda, Y.Yoshida, B.E.Shan, U.Yamashita: "Suppression of macrophage interleukin-12 and tumour necrosis factor-α production in mice infected with Toxocar canis"Parasite Immunology. 23. 305-311 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Okada, T.Sugiura, E.Kuroda, S.Tsuji, U.Yamashita: "Phenytoin promotes Th2 type immune response in mice"Clinical and Experimental Immunology. 124. 406-413 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Okada, S.Tsuji, U.Yamashita: "Cyclooxygenase-2 expression in the hippocampus from genetically epilepsy susceptible El mice"Brain Research. 894. 332-335 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] E.Kuroda,T.Sugiura,K.Zeki,Y.Yoshida,U.Yamashita: "Sensitivity difference to the suppressive effect of prostaglandin E_2 among mouse strains : A possible mechanism to polarize Th2 type response in BALB/c mice."J.Immunology. 164. 2386-2395 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Kawakami,N.Nagai,K.Ohama,K.Zeki,Y.Yoshida,E.Kuroda,U.Yamashita: "Macrophage-colony stimulating factor inhibits the growth of human ovarian cancer cells in vitro."European J.Cnacer. 36. 1991-1997 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Okada,Y.Yoshida,T.Sugiura,H.Tanaka,S.Tsuji,H.Yamashita,U.Yamashita: "Reduced interleukin-1 responsiveness in immune system and central nervous system of inbred polydipsic (STR/N) mice."Life Sciences. 99. 1461-1470 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Yamada,S.Matsumoto,T.Matsumoto,T.Yamada,U.Yamashita: "Enhancing effect of an inhibitor of nitric oxide synthesis on Bacillus Calmette-Guerin-induced macrophage cytotoxicity against murine bladder cancer cell line MBT-2 in vitro."Japanese J.Cancer Research. 91. 534-542 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Yamada,S.Matsumoto,T.Matsumoto,T.Yamada,U.Yamashita: "Murine IL-2 secreting recombinant Bacillus Calmette-Guerin augments macrophage-mediated cytotoxity against murine bladder cancer MBT-2."J.Urology. 164. 526-531 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] B.E.Shan,K.Zeki,T.Sugiura,Y.Yoshida,U.Yamashita: "Chsinese medicinal herb, Acanthopanax gracilistlus, extract induces cell cycle arrest of human tumor cells in vitro."Japanese J.Cancer Research. 91. 383-389 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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