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Experimental study of effects of vitamin K2 for prevention of osteoporosis.

Research Project

Project/Area Number 12670372
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Public health/Health science
Research InstitutionKAWASAKI MEDICAL SCHOOL

Principal Investigator

KATSUYAMA Hironobu  Kawasaki Medical School,Medicine, Associate Professor, 医学部, 講師 (00289175)

Co-Investigator(Kenkyū-buntansha) IDEGUCHI Seiji  Kawasaki Medical School, Medicine, Associate Professor, 医学部, 助教授 (70168334)
FUKUNAGA Masao  Kawasaki Medical School, Medicine, Professor, 医学部, 教授 (00093302)
SUNAMI Shigeo  Kawasaki Medical School, Medicine, Professor, 医学部, 教授 (20069040)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Keywordsvitamin K_2 / osteocalcin / RANK / RANKL / osteoprotegerin / real time PCR / SSH / RT-PCR
Research Abstract

Previous study showed that nuclear binding proteins to vitamin K2 were found in osteoblast. In order to clarify the action of vitamin K2 on osteoblastic function, various concentrations of vitamin K 2 were administered to MC3T3E1 cells, which originated in mouse osteoblasts. Concentrations of 10 7M to 10 5M of vitamin K2 were administered to MC3T3E1 cells not only vitamin K2 alone but also with 10 8M of activated vitamin D. In cell growth, numbers of cells administered various vitamins were higher than that of control cells. Therefore, we investigated the effects サof vitamin K2 on osteocalcin mRNA, receptor activator of NFkB CNK) mRNA, RANK ligand (RANKL) mRNA and osteoprotegerin mRNA. These mRNA levels were increased at 1 hour and 6 after vitamin K2 administration at concentration of 10 6M. Same tendency was observed in addition of vitamin D. Since vitamin K2 seemed to affect cell function, suppression subtractive hybridization method (S SH) were used to detect the factors which might affect cell function. In this method, we detected the genes such as tenascin, lumican, etc. Further investigation is needed to detect the genes which is affected by vitamin K2.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 勝山博信: "マウス骨芽細胞に対するビタミンK_2投与の影響"日本衛生学雑誌. 57巻・1号. 378 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuyama, H., Ideguchi, S., Fukunaga, M., Saijoh, K., Sunami, S.: "Influence of vitamin K_2 intake on bone turnover."Jpn. J. Hygiene. 54-1. 119-119 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuyama, H., Ideguchi, S., Fukunaga, M., Saijoh, K., Sunami, S.: "Biochemical markers for bone turnover and vitamin K_2 intake."abstract for EXPERIMENTAL BIOLOGY '99 (at Washington, DC., USA ; 17-21 April 1999). -449

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuyama, H., Ideguchi, S., Fukunaga, M., Saijoh, K., Sunami, S.: "Effects of vitamin K_2 intake and vitamin D receptor polymorphism on bone turnover."Jpn. J. Hygiene. 55-1. 449-162 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuyama, H., Ideguchi, S., Fukunaga, M., Saijoh, K., Sunami, S.: "Cross sectional study of influence of vitamin K_2 intake on bone mineral loss."J. Epidemiol. 11-1. 162-123 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuyama, H., Ideguchi, S., Fukunaga, M., Saijoh, K., Sunami, S.: "Effects of continuous intake of vitamin K_2 on bone turnover."Jpn. J. Hygiene. 56-1. 123-109 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuyama, H., Ideguchi, S., Fukunaga, M., Saijoh, K., Sunami, S.: "Influence of vitamin K_2 intake on bone turnover markers."J. Epidemiol. 12-1. 109-378 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuyama, H., Ideguchi, S., Fukunaga, M., Saijoh, K., Sunami, S.: "Influence of vitamin K_2 administration on mouse osteoblast"Jpn. J. Hygiene. 57-1. 378 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 勝山 博信他: "マウス骨芽細胞に対するビタミンK_2投与の影響"日本衛生学雑誌. 57・1. 378 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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