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Analysis of the effect of stress on the gut-associated immune systems by the genetic method

Research Project

Project/Area Number 12670388
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Public health/Health science
Research InstitutionOsaka Prefectural Institute of Public Health

Principal Investigator

NAKANO Yumiko  Division of occupational health Chief research worker, 労働衛生部, 主任研究員 (40159067)

Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsInflammatory bowel disease / Stress / Trinitrochlorobenzene / Gene expression / DNA array / Cytokine / RT-PCR / 大腸 / Th_1 / Th_2 / ホルモン / 神経伝達物質 / RT・PCR / 単独融離 / 遺伝子発現解析 / 神経ペプチド / トリニトロクロルベンゼン / がん原遺伝子 / アポトーシス
Research Abstract

It is reported that stress is deeply concerned with the development of intestinal diseases, such as large intestine cancer, an inflammational bawel disease, and irritable colon syndrome. Therefore, we investigated the effect of the isolation stress on the gut-associated lymphoid system. ConA responses of mesenteric lymph node cells were reinforced at the acute phase of the stress, and was suppressed at the chronic phase. T/B ratio was enhanced at the acute phase, and suppressed at the chronic phase. The production of IL-4 was markedly enhanced at the chronic phase, showing the possibility of Th2 type-dominant. Thus, the mesenteric lymph node cells were notably influenced by the stress, though the spleen cells exhibited the inverse relationship. Serum cortisone levels were upregulated in acute phase, while substance P levels were upregulated in chronic phase.
The influence of stress on the large intestine was analyzed in the gene discovery analysis using DNA array. Gene's upregulated sig … More nificantly compared to control were most abundant in chronic phase. Reduction of IL- 12 production and upregulation of IL- 10 and its receptor suggested that the stress changes the Th1/Th2 balance to Th2. In the sample from 2 and 30 day isolation, many genes related to hormone, neurotransmitter, and their receptors were upregulated. These results indicated that isolation stress profoundly affects the large intestine, and made it to the neuronal hypersensitivity.
Next experiments were performed using chronic ulcerative colitis-induced mice by trinitrochlorobenzene (TNBS) under isolation stress load for 30 days or not. RNA was extracted from the large intestines and gene expression analysis was performed. As a result, the transcription factor & DNA-binding proteins related genes were upregulated in TNBS treated mice. The ion channels & transport proteins related genes were upregulated in mice treated with TNBS and loaded with stress. Thus, stress increases and modulates gene expression in large intestine. These results were ascertained by RT-PCR using the real-time PCR. Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Nakano Y, Ando K, Nakamura S, et al.: "Relationships between lifestyle-related factors and immune parameters in middle-aged male workers"J Occup Health. 43. 321-330 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 中野ユミ子: "中高齢者の健康実態調査報告・免疫機能は健康のバロメーター"ESTRELA. 78. 46-51 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 中野ユミ子, 吉田仁: "遺伝子発現プロファイルによる感作性物質の分類"Allergology. 14. 310-318 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakano Y: "Stress induced modulation of skin immune function : Two types of antigen presenting cells in the epidermis are differentially regulated by chronic stress"Brit.J.Dermatol. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakano Y, Ando K, Nakamura S, et al.: "Relationships between lifestyle-related factors and immune parameters in middle-aged male workers"J Occup Health. 43. 321-330 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakano Y.: "A healthy survey report of middle and old age people ; Immunity function is the health barometer"ESTRELA. 78. 46-51 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakano Y. and Yosida J.: "Classification of the contact sensitizers by the gene expression profile"Allergology. 14. 310-318 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakano Y.: "Stress induced modulation of skin immune function : Two types of antigen presenting cells in the epidermis are differentially regulated by chronic stress"Brit. J. Dermatol. Inpress.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakano Y, Ando K, Nakamura S, et al.: "Relationships between lifestyle-related factors and immune parameters in middle-aged male workers"J Occup Health. 43. 321-330 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中野ユミ子: "中高齢者の健康実態調査報告・免疫機能は健康のバロメーター"ESTRELA. 78. 46-51 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中野ユミ子・吉田仁: "遺伝子発現プロファイルによる感作性物質の分類"Allergology. 14. 310-318 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakano Y: "Stress induced modulation of skin immune function : Two types of antigen presenting cells in the epidermis are differentially regulated by chronic stress"Brit.J.Dermatol. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakano Y., Ando K.et al.: "Relationships between life-related factory and Immune parameters in Middle-Aged Male Workers"J Occup Health. 43. 321-330 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yumiko NAKANO^1,Katashi ANDO^1,Seiichi NAKAMURA^1, 他: "Relationships between lifestyle-related factors and immune parameters in middle-aged male workers"Journal of Occupational Health. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 中野ユミ子: "中高齢者の健康実態調査報告・免疫機能は健康のバロメーター"ESTRELA. 78. 46-51 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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