| Project/Area Number |
12670422
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
OHASHI Hiroyuki Hamamatsu University School of Medicine, Department of Internal Medicine III, Associate Professor, 医学部附属病院, 講師 (60233243)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Yasuhiro Hamamatsu University School of Medicine, Department of Internal Medicine III, Assistant Professor, 医学部附属病院, 講師 (10334971)
赤嶺 紀子 浜松医科大学, 医学部, 助手 (20322141)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Sjogren's syndrome / CD40 / CD40L(CD154) / Accessory molecules / Cytokine / Costimulator |
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| Research Abstract |
CD40-CD40 Ligand (CD40L) costimulation has been shown to play a major role in regulating both humoral and cellular immune responses. In this experiments we evaluated the role of CD40-CD40L system in patients with SS. Methods : 1. Serum samples from 33 primary SS (I°SS) patients, 37 secondary SS (II°SS) patients and 40 healthy controls were measured by sCD40L ELISA kits. 2. Their peripheral blood mononuclear cells (PBMC) were stained and analyzed by two-color flowcytometry. 3. Isolated PBMC were cultured and stimulated by ionomycin and PMA because of detection for CD40L expression. 4. The labial salivary glands from SS patients were immunohistochemically stained by anti-CD40, CD40L, CD3, CD4, CD8 monoclonal antibodies. 5. Total RNA was extracted from labial salivary glands. CD40 and CD40L mRNA expression in salivary glands were detected by RT-PCR method. Results : 1. The concentration of serum CD40L was statistically significant higher (P<0.01) in both I°SS and II°SS patients than in healthy controls. 2. There was no significant difference between healthy controls and SS patients in the expression of CD40L on both T cells and B cells. 3. Cultured T cells by Ionomycin and PMA continued to demonstrate the increased expression of CD40L in primary SS rather than normal controls at 48 hours. 4. Immunohistochemical analyses of SS salivary glands showed that a minor but clear expression of CD40L was present on infiltrating mononuclear cell in the salivary glands from SS patients. 5. The expression of CD40 and CD40L mRNA were detected in the salivary glands from SS patients but not from normal controls. Conclusion : The activation of CD40-CD40L system may play a role of a pathogenesis of polyclonal B cell activation in Sjogren's syndrome.
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