| Project/Area Number |
12670425
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
NAKATA Yasunari Medical School, Professor, 医学部, 教授 (80112150)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Shuji Graduate School of Medicine and Dentistry, Assi. Professor, 大学院・医歯学総合研究科, 助教授 (50220009)
TOGE Hiroko Medical School, ssi. professor, 医学部, 助教授 (10197840)
KATAOKA Mikio Medical School, Professor, 医学部, 教授 (50177391)
SAKIYAMA Junko Medical School, Assistant, 医学部, 助手 (40204599)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | sarcoidosis / Propionibacterium acnes / lymphocyte / サイコイドーシス / プロピオニバクテリウムーアクネス |
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| Research Abstract |
Despite the extensive investigations into the pathogenesis of sarcoidosis, the etiology of sarcoidosis is still unknown. In previous studies, Honma H. and his colleagues found Propionibacterium acnes (P. acnes) in the affected lymph nodes of sarcoidosis patients. In this study, we investigated to detect DNA of P. acnes in cells recovered from bronchoalveolar lavage (BAL) in patients with sarcoidosis. We applied a nested polymerase chain reaction (PCR) assay and in situ PCR hybridization as a tool to detect DNA of P. acnes. The PCR-technique is based on the amplification of P. acnes small subunit ribosomal RNA. BAL cells from thirty patients with histologically proven sarcoidosis and 30 controls with other pulmonary diseases were underwent assay by specifically detected DNA from P. acnes. DNA of P. acnes was detected in 70% in the BAL cells with sarcoidosis patients and 23.3% in the controls, the proportion of sarcoidosis is significantly greater than that of controls (p<0.001). By in situ PCR hybridization, signal of P. acnes DNA was detected only in cytoplasm of alveolar macrophages from sarcoidosis, but not from non-sarcoidosis patients.
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