| Project/Area Number |
12670432
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
|
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
SATO Yukio Fukushima Medical University School of Medicine, Professor of Medicine, 医学部, 講師 (20254013)
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| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | DNA VACCINE / MCROLIDE ANTIBIOTIC / ALLERGY / EOSINOPHILIA |
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| Research Abstract |
We examined the effect of erythromycin (EM), a 14-membered macrolide, on the immune response to a DNA vaccine that induces a T-helper-1 (Thl)-biased immune response through a Thl-promoting adjuvant effect of unmethylated CpG motifs within plasmid DNA. EM enhanced Thl responses in plasmid DNA-immunized mice as measured by antigen-specific IgG2a antibody production, interferon- γ production by antigen-specific CD4+ T cells, and cytotoxic T lymphocyte responses. Since immunization with plasmid DNA encoding a specific allergen via the systemic routes has been shown to both prevent activation of allergen-specific Th2 cells following injection of allergen protein in aluminum hydroxide, we attempted to determine whether EM enhances the inhibition of pulmonary eosinophilia in ovalbumin (OVA)-sensitized mice after inhalation OVA challenge. The results showed that EM enhances the inhibitory effect of DNA vaccine on pulmonary eosinophilia.
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