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Investigation into the roles of autoantibodies in the pathogenesis of central nervous system lupus erythematosus.

Research Project

Project/Area Number 12670438
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionTeikyo University School of Medicine

Principal Investigator

HIROHATA Shunsei  Teikyo University, Internal Medicine, Associate Professor, 医学部, 助教授 (90189895)

Co-Investigator(Kenkyū-buntansha) KIKUCHI Hirotoshi  Teikyo University, Internal Medicine, Assistant Professor, 医学部, 助手 (80338681)
HIROSE Naoto  Teikyo University, Internal Medicine, Assistant Professor, 医学部, 助手 (60261971)
MIYASHITA Taku  Teikyo University, Internal Medicine, Assistant Professor, 医学部, 助手 (00239401)
柳田 たみ子  帝京大学, 医学部, 助手 (80082204)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsSLE / CNS lupus / anti-ribosomal P antibody / monocyte / anti-lymphocyte antibody / glutamate receptor / anti-neuronal antibody / cerebrospinan fluid / Tリンパ球 / Bリンパ球 / 固相化抗CD3抗体
Research Abstract

We examined the presence of the epitope recognized by antiribosomal P protein antibody (anti-P) on the cell surface of human immunocompetent cells in order to delineate its role in the pathogenesis of CNS lupus. Highly purified CD4+ and GD8+ T cells, monocytes, and B cells from normal healthy individuals were reacted with affinity-purified IgG anti-P, and were stained with FITC-conjugated F(ab')_2, fragment goat anti-human IgG, followed by analysis on flow cytometry with gating for viable cells by propidium iodide staining. The presence of an epitope that is antigenically related to the earboxyl-terminal 22-amino-acid sequence of ribosomal P protein was not demonstrated on the surface offreshCD4+ and CD8+ T cells, monocytes, or fresh B cells. However, the expression of the ribosomal P epitope was induced on CD4+ and CD8+ T cells after activation with immobilized anti-CD3 and on monocytes after 24h incubation with on without IFN-γ, whereas the epitope was not expressed on activated B cells. These results indicate that anti-P is an antilymphocyte' antibody, which reacts with activated T cells and monocytes but not with resting T cells, monocytes or B cells, suggesting possible direct effects of anti-P on the immune dysregulation in systemic lupus erythematosus. We could not detect significant elevation of anti-glutamate receptor antibody in cerebrospinal fluid from patients with lupus psychosis, indicating that recognized by anti-neuronal antibody.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Hirohata S: "Systemic sclerosis complicated with nephrotic syndrome : relevance with antiribosomal P antibody"Rheumatology Int.. 21. 40-43 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirohata S: "Induction of fibroblast-like cells from CD34+ progenitor cells of the bone marrow in rheumatoid arthritis"J.Leukoc.Biol.. 70. 413-421 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirohata S: "Antiribosomal P protein antibody in human systemic lupus erythematosus reacts specifically with activated T cells"Lupus. 10. 612-621 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirohata S: "Regulation of human B cell function by sulfasalazine and its metabolites"International Immunopharmacology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirohata S: "Bone marrow CD34+ progenitor cells stimulated with stem cell factor and GM-CSF have the capacity to activate IgD-B cells through direct cellular interaction"J.Leukoc.Biology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 小嶋浩司: "ポリニューロパチーを合併した抗リン脂質抗体陽性のSLEの1例"日内会誌. 90. 136-137 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 広畑俊成: "リウマチナビゲーター"メディカルレビュー社. 296 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 広畑俊成: "看護のための最新医学講座 第11巻 免疫・アレルギー疾患"中山書店. 364 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirohata S.: "Systemic sclerosis complicated with nephrotic syndrome : relevance with antiribosomal P antibody"Rheumatology Int.. 21. 40-43 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirohata S.: "Induction of fibroblast-like cells from CD34+ progenitor cells of the bone marrow in rheumatoid arthritis"J. Leukoc. Biol.. 70. 413-421 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirohata S.: "Antiribosomal P protein antibody in human systemic lupus erythematosus reacts specifically with activated T cells"International Immunopharmacology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirohata S.: "Bone marrow CD34+ progenitor cells stimulated with stem cell factor and GM-CSF have the capacity to activate IgD- B cells through direct cellular interaction"J. Leukoc. Biology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirohata S: "Systemic sclerosis complicated with nephrotic syndrome : relevance with antiribosomal P antibody"Rheumatology Int.. 21. 40-43 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirohata S: "Induction of fibroblast-like cells from CD34+ progenitor cells of the bone marrow in rheumatoid arthritis"J. Leukoc. Biol.. 70. 413-421 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirohata S: "Antiribosomal P protein antibody in human systemic lupus erythematosus reacts specifically with activated T cells"Lupus. 10. 612-621 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirohata S: "Regulation of human B cell function by sulfasalazine and its metabolites"International Immunopharmacology. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirohata S: "Bone marrow CD34+ progenitor cells stimulated with stem cell factor and GM-CSF have the capacity to activate IgD-B cells through direct cellular interaction"J. Leukoc. Biology. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 小嶋浩司: "ポリニューロパチーを合併した抗リン脂質抗体陽性のSLEの1例"日内会誌. 90. 136-137 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 広畑俊成: "リウマチナビゲーター"メディカルレビュー社. 296 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirohata S: "Bone marrow CD34+ progenitor cells from rheumatoid arthritis patients support spontaneous transformation of peripheral blood B cells from Healthy individuals."Rheumatol Int. 19. 153-159 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirohata S: "Inhibition of cyclin A gene expression in human B cells by an immunosuppressant mizoribine."Clin Exp Immunol. 120. 448-453 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamashita S: "Analysis of neutrophil proteins of patients with Behcet's disease by two-dimensionnal gel elecreophoresis."Biol Pharm Bull. 23. 519-522 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawai M: "Cerebrospinal fluid β2-microglobulin in neuro-Behcet's syndrome."J Neurol Sci. 179. 132-139 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 広畑俊成: "特集:免疫性神経疾患 -ここまで進んだ病態解明と治療全身性エリテマトーデスに伴う神経障害<その他の自己免疫機序による神経疾患>"内科. 4. 687-691 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 広畑俊成: "第VIII編 各種疾患にみられる神経疾患 E.リウマチ性疾患 1.全身性エリテマトーデス(SLE)"別冊日本臨床 領域別症候群シリーズNo.29 神経症候群IV -その他の神経疾患を含めて. 29. 261-264 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 広畑俊成: "膠原病診療 専門医によるベストアドバイス -困ったときにどう考えるか"診断と治療社. 216 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 広畑俊成: "コンパクト臨床アレルギー学"南江堂. 370 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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