Immunotherapy by dendritic cells against hepatocellular carcinoma
| Project/Area Number |
12670495
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Ehime University |
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Principal Investigator |
ONJI Morikazu Ehime University, Faculty of Medicine, Professor, 医学部, 教授 (10112260)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | hepatocellular carcinoma / dendritic cells / immune therapy / CTL |
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| Research Abstract |
Defective function and impaired maturation of immune regulatory cells, especially antigen-presenting dendritic cells (DC) have been found in patients with malignancies including hepatocellular carcinoma (HCC) (1-4), indicating that there is a requirement of development of immune therapy targeting DC in malignancies. In most cases, anti tumor therapy has been done by administrating tumor antigen-laden DC in to the systemic circulation. Here, we report that when only bone marrow-derived immature DCs were administered directly in to the implanted colorectal carcinoma of C57BL/6 mice, there was neither significant maturation of DC nor anti tumor activity. However, when immature DCs were administered 48 hours after necrotization of colorectal carcinoma by ethanol, many mature DC expressing CD86 antigen were localized in and around the cancer nodules. Most importantly, accumulation of mature DCs at the cancer nodules was followed by reduction of the volume of the cancer nodules. This study indicate that immature DC can be induced to undergo maturation at the cancer mirco environment by ethanol. As ethanol is injected in to HCC nodule as a local therapy in patients with HCC, this study inspire optimism that local administration of immature DC followed by ethanol injection may be a new and effective therapeutic tool for the treatment of HCC in human.
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Report
(3 results)
Research Products
(13 results)
