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CELL-TO-CELL INTERACTION IN THE MARGINALSINUS OF MESENTERIC LYMPH NODE

Research Project

Project/Area Number 12670515
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKeio University

Principal Investigator

NAGATA Hiroshi  KEIO UNIVERSITY, MEDICINE, ASSOCATE PROFESSOR, 医学部, 助教授 (00146599)

Co-Investigator(Kenkyū-buntansha) MINAMITANI Haruyuki  KEIO UNIVERSITY, ENGINEERING, PROFESSOR, 理工学部, 教授 (70051779)
SUEMATSU Makoto  KEIO UNIVERSITY, MEDICINE, PROFESSOR, 医学部, 教授 (00206385)
SUZUKI Hidekazu  KEIO UNIVERSITY, MEDICINE, INSTRUCTOR, 医学部, 助手 (70255454)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordslymph node / marginal sinus / lymhphocyte hhoming / macrophage / cancer metastasis / dendritic cell / cell-to-cell interaction / 辺緑洞
Research Abstract

1) Distribution of lymphatic microvessels and migration of lymphocyte in the mesenteric lymph node of rat
Labeled lymphocytes in blood adhered to Peyer's patch venules preferentially. The lymphocytes transmigrated from the venules and entered efferent lymphatics in Peyer's patches, and the flowed into the lymph node. The lymphocytes were arrested in the marginal sinus, which has huge surface sufficient for lymphocytes pooling. It is indicated that lymphocytes homing to mesenteric lymph nodes predominantly passes through afferent lymphatics.
2) Visualization of macrophage migration in mesenteric lymph node
Fluorescent particles were taken up by EDS positive macrophages beneath the marginal sinus. The number of the particleladen macrophages seen by epi-illumination increased up to 12 hr after injection, and then declined. Some particle-laden macrophages entered the marginal sinus and flowed rapidly toward the hilus of the lymph node. In cryostat sections, the particle-laden macrophages tran … More smigrated through the cortex toward the medulla in course of time. Upon phagocytosis of foreign bodies, the ED3 positive macrophages migrate toward the medulla through the marginal sinus or cortex to find immunoproliferative microenvironment.
3) Experimental animal model of early lymph node metastasis and response of antigen presenting cells.
Fluorescence labeled cancer cells (AH 130 ascites hepatoma cells) were arrested in the marginal sinus of the meso-cecum lymph node. The number of cancer cells, flowed into the marginal sinus, reached the maximum 6 hours after injection. The marginal sinus was filled with cancer cells and expanded 4days after injection. Beneath the cancer cell linings, ED 3 positive macrophages have accumulated. They incorporated apoptotic cancer cells. The dendritic cell, resided in the paracortex, did not accumulate, but distributed in small number in the rim of the cancer cell cluster. In lymph nodes depleted of macrophages, the cancer cells invaded early to the paracortex. Macrophages beneath the marginal sinus play a role in the first line defense against cancer proliferation and invasion.
4) Migration of dendritic cells from the gut to lymph node.
Labeled dendritic cells were seen to migrate to the mesenteric lymph node 6 hours after injection to the cecum submucosa, and reached the maximum in number at 48 hours, and then declined at 72 hours. Cytokines stimulation in vitro upregulated the expression of MHC class II of dendritic cells, resulting in early transfer to the lymph node. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Nagata Hiroshi: "In vivo observation of phagocytosis and migration of subsinus macrophge in the mesenteric lymph node"In : Proceedings of 21st European Conference on Microcirculation, edited by B.Fagrel. 61-65 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagata Hiroshi: "Lymphocyte homing parhway to mesenteric lymph node"Microcirculation Annual. 16. 83-84 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagata Hiroshi: "In vivo identification of parasinus macrophage in the mesenteric lymph node"Clinical Hemorheology and Microcirculation. 23. 345-348 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hokari Ryota: "Intercellular cell adhesion molecule-1 regulates movement into intestinal microlymphatics of rat Peyeris patches"J Leukocyte Biol. 70. 896-902 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hokari Ryota: "Involvement of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in the pathogenesis of granulomatous colitis in rats"Clin Exp Immunol. 126. 259-265 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagata Hiroshi: "Visualization of macrophage migration in mesenteric lymph node of rat"Microcirculation annual. 17. 139-140 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagata H et al: "In vivo observation of phagocytosis and migration of subsmus macrophge in the mesenteric lymph node"In: Proceedings of 21st European Conference on Microcirculation. edited by B. Fagrel. 61-65 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagata H et al: "Lymphocyte homing pathway to mesenteric lymph node"Microcirculation Annual. 16. 83-84 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagata H et al: "In vivo identification of parasinus macrophage in the mesenteric lymph node"Clinical Hemorheology and Microcirculation. 23. 345-348 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hokari R et al: "Intercellular cell adhesion molecule-1 regulates movement into intestinal microlymphatics of rat Payer's patches"J Leukocyte Biol. 70. 896-902 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hokari R et al: "Involvement of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in the pathogenesis of granulomatous colitis in rats"Clin Exp Immunol. 126. 259-265 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagata H et al: "Visualization of macrophage migration in mesenteric lymph node of rat"Microcirculation annual. 17. 139-140 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koseki S. et al.: "In situ demonstration of intraepithelial lymphocyteadhesion to villus microvessels of the small intestine"International Immunology. 13. 1165-1174 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hokari Ryota: "Intercellular cell adhesion molecule-1 regulates movement into intestinal microlymphatics of rat Peyer's patches"JLeukocyte Biol. 70・6. 896-902 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hokari Ryota: "Involvement of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in the pathogenesis of granulomatous colitis in rats"Clin Exp Immunol. 126・2. 259-265 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nagata Hiroshi: "Visualization of macrophage migration in mesenteric lymph node of rat"Microcirculation annual. 17. 139-140 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Watanabe C: "Intravital observation of T-lymphocytes in the colonic microcirculation"Microcirculation annual. 17. 137-138 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 永田博司: "胃粘膜障害と微小循環障害-神経性ペプチドの関与とアルコールの影響"日医雑誌. 125・7. 10-13 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nagata Hiroshi: "In vivo observation of phagocytosis and migration of subsinus macrophge in the mesenteric lymph node"Proceedings of 21st European Conference on Microcirculation, edited by B.Fagrel. 61-65 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nagata Hiroshi: "Lymphocyte homing pathway to mesenteric lymph node"Microcirculation Annual. 16. 83-84 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 原由里子: "脂肪酸鎖長の相違による小腸上皮間リンパ球IFN-γ産生への影響"消化と吸収. 23. 41-45 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 岸川浩: "物理的圧力負荷による小腸上皮細胞の増殖およびサイトカイン放出の検討"Cytoprotection & biology. 18. 49-51 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 鈴木秀和: "酸化ストレスとアポトーシス"GI Research. 8. 164-176 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nagata Hiroshi: "In vivo identification of parasinus macrophage in the mesenteric lymph node"Clinical Hemorheology and Microcirculation. 22(発表予定). (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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